Intracellular cytokine optimization and standard operating procedure
Tóm tắt
Từ khóa
Tài liệu tham khảo
Chattopadhyay, P.K. & Roederer, M. Immunophenotyping of T cell subpopulations in HIV disease. in Current Protocols in Immunology (eds. Coligan, J.E., Bierer, B., Margulies, D.H., Shevach, E.M. & Strober, W.) 12.12.1–12.12.15 (John Wiley & Sons, Hoboken, New Jersey, USA, 2005).
Maecker, H.T. Cytokine flow cytometry. Methods Mol. Biol. 263, 95–108 (2004).
Suni, M.A. et al. Performance of plate-based cytokine flow cytometry with automated data analysis. BMC Immunol. 4, 9 (2003).
Catanzaro, A. et al. Phase I safety and immunogenicity evaluation of a multiclade HIV-1 recombinant adenoviral vector vaccine in uninfected adults. J. Infect. Dis. (in the press).
Graham, B. et al. A multiclade HIV-1 DNA vaccine elicits humoral and sustained cellular immune responses in humans in a randomized controlled Phase I clinical trial. J. Infect. Dis. (in the press).
De Rosa, S.C. et al. Vaccination in humans generates broad T cell cytokine responses. J. Immunol. 173, 5372–5380 (2004).
Betts, M.R. et al. Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J. Immunol. Methods 281, 65–78 (2003).
Betts, M.R. & Koup, R.A. Detection of T-cell degranulation: CD107a and b. Methods Cell Biol. 75, 497–512 (2004).
Rubio, V. et al. Ex vivo identification, isolation and analysis of tumor-cytolytic T cells. Nat. Med. 9, 1377–1382 (2003).
Pittet, M.J., Speiser, D.E., Valmori, D., Cerottini, J.C. & Romero, P. Cutting edge: cytolytic effector function in human circulating CD8+ T cells closely correlates with CD56 surface expression. J. Immunol. 164, 1148–1152 (2000).
Perfetto, S. Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. J. Immunol. Methods 313, 199–208 (2006).
Appay, V. et al. Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infections. Nat. Med. 8, 379–385 (2002).
De Rosa, S.C., Herzenberg, L.A., Herzenberg, L.A. & Roederer, M. 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat. Med. 7, 245–248 (2001).
Roederer, M. et al. CD8 naive T cell counts decrease progressively in HIV-infected adults. J. Clin. Invest. 95, 2061–2066 (1995).
Sallusto, F., Lenig, D., Forster, R., Lipp, M. & Lanzavecchia, A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401, 708–712 (1999).
Perfetto, S.P., Chattopadhyay, P.K. & Roederer, M. Seventeen-colour flow cytometry: unravelling the immune system. Nat. Rev. Immunol. 4, 648–655 (2004).
Wu, X. & Bruchez, M.P. Labeling cellular targets with semiconductor quantum dot conjugates. Methods Cell Biol. 75, 171–183 (2004).
Chattopadhyay, P.K. et al. Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow. Nat. Med. 12, 972–977 (2006).
Darzynkiewicz, Z., Li, X. & Gong, J. Assays of cell viability: discrimination of cells dying by apoptosis. Methods Cell Biol. 41, 15–38 (1994).
O'Neil-Andersen, N.J. & Lawrence, D.A. Differential modulation of surface and intracellular protein expression by T cells after stimulation in the presence of monensin or brefeldin A. Clin. Diagn. Lab. Immunol. 9, 243–250 (2002).
Roederer, M. Compensation is not dependent on signal intensity or on number of parameters. Cytometry 46, 357–359 (2001).
Roederer, M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry 45, 194–205 (2001).
Cherepnev, G., Volk, H.D. & Kern, F. Use of peptides and peptide libraries as T-cell stimulants in flow cytometric studies. Methods Cell Biol. 75, 453–479 (2004).
Mollenhauer, H.H., Morre, D.J. & Rowe, L.D. Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity. Biochim. Biophys. Acta 1031, 225–246 (1990).