Intraarticular injection of platelet‐rich plasma reduces inflammation in a pig model of rheumatoid arthritis of the knee joint

Wiley - Tập 63 Số 11 - Trang 3344-3353 - 2011
Sebastian Lippross1, B Moeller1, Holger Haas1, Mersedeh Tohidnezhad2, Nadine Steubesand1, Christoph Jan Wruck2, Bodo Kurz3,4, Andreas Seekamp1, Thomas Pufe2, Deike Varoga1
1University Medical Center Schleswig Holstein, Kiel Campus, Kiel, Germany
2RWTH-Aachen University, Aachen, Germany.
3Bond University, Gold Coast, Queensland, Australia
4Christian-Albrecht University of Kiel, Kiel, Germany

Tóm tắt

AbstractObjectiveTreatment options for rheumatoid arthritis range from symptomatic approaches to modern molecular interventions such as inhibition of inflammatory mediators. Inhibition of inflammation by platelet‐rich plasma (PRP) has been proposed as a treatment for tendinitis and osteoarthritis. The present study was undertaken to investigate the effect of PRP on antigen‐induced arthritis (AIA) of the knee joint in a large animal model.MethodsSix‐month‐old pigs (n = 10) were systemically immunized by bovine serum albumin (BSA) injection, and arthritis was induced by intraarticular BSA injection. PRP was injected into the knee joints of 5 of the animals after 2 weeks. An additional 5 animals received no systemic immunization (controls). Signs of arthritis were documented by plain histologic analysis, Safranin O staining, and immunohistochemistry analysis for type II collagen (CII), interleukin‐6 (IL‐6), and vascular endothelial growth factor (VEGF). Interleukin‐1β (IL‐1β), IL‐6, tumor necrosis factor α (TNFα), VEGF, and insulin‐like growth factor 1 (IGF‐1) protein content was measured by Luminex assay.ResultsIn the pigs with AIA, plain histologic analysis revealed severe arthritic changes in the synovium. Safranin O and CII staining showed decreased proteoglycan and CII content in cartilage. Immunohistochemistry analysis revealed increased levels of IL‐6 and VEGF in synovium and cartilage, and protein concentrations of IL‐6, VEGF, IL‐1β, and IGF‐1 in synovium and cartilage were elevated as well; in addition, TNFα protein was increased in cartilage. Treatment with PRP led to attenuation of these arthritic changes in the synovium and cartilage.ConclusionWe have described a porcine model of AIA. Experiments using this model demonstrated that PRP can attenuate arthritic changes as assessed histologically and based on protein synthesis of typical inflammatory mediators in the synovial membrane and cartilage.

Từ khóa


Tài liệu tham khảo

10.1038/nri2094

10.1016/S0140-6736(89)90430-3

10.1002/j.1460-2075.1991.tb04978.x

Shrive NG, 1978, Load‐bearing in the knee joint, Clin Orthop Relat Res, 279

10.1002/art.27186

10.1186/ar1689

10.1073/pnas.95.14.8222

Pufe T, 2001, Splice variants VEGF121 and VEGF165 of the angiogenic peptide vascular endothelial cell growth factor are expressed in the synovial tissue of patients with rheumatoid arthritis, J Rheumatol, 28, 1482

10.4049/jimmunol.152.8.4149

10.1136/ard.54.8.645

10.1160/TH03-07-0440

10.1080/00016470410001708190

10.1177/107110070502600606

10.1055/s-2002-19824

10.1007/BF02848692

10.1016/S0014-4800(03)00017-0

Driver VR, 2006, A prospective, randomized, controlled trial of autologous platelet‐rich plasma gel for the treatment of diabetic foot ulcers, Ostomy Wound Manage, 52, 68

10.1302/0301-620X.89B3.18491

10.1016/j.injury.2010.12.010

10.1097/01.PRS.0000138251.07040.51

10.1054/jcms.2002.0285

10.3727/000000003108747073

10.1177/0363546506288850

10.1089/ten.tec.2008.0534

10.1016/S0030-5898(05)70117-6

10.1016/j.cden.2005.11.007

10.2106/00004623-199506000-00016

10.1097/00003086-199810001-00010

10.1146/annurev.bioeng.1.1.19

10.1111/j.1582-4934.2005.tb00338.x

10.1016/S0142-9612(03)00114-5

10.1007/b99997

10.1002/mabi.200400026

10.1073/pnas.152324099

Sanchez M, 2008, Intra‐articular injection of an autologous preparation rich in growth factors for the treatment of knee OA: a retrospective cohort study, Clin Exp Rheumatol, 26, 910

10.1007/s00167-009-0940-8

10.1538/expanim.45.299

10.1002/art.1780170612

10.1002/art.23660

10.1016/0076-6879(88)62091-X

Sokoloff L, 1973, Animal model: arthritis due to Mycoplasma in rats and swine, Am J Pathol, 73, 261

10.1073/pnas.0907550106

10.1136/ard.2010.132720

10.1016/j.bone.2003.08.002

10.1002/path.2059

10.1097/00005373-199112000-00017

10.1002/art.1780360107

10.1002/art.1780390916

10.1002/1529-0131(200011)43:11<2563::AID-ANR25>3.0.CO;2-8

10.1002/art.1780370215

10.1016/S0083-6729(06)74016-X

Thông tin
Thông tin xuất bản

Wiley

Tập 63 Số 11

3344-3353

Thông tin tác giả