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Sự tương tác giữa Axit Abscisic và Axit Jasmonic và vai trò của chúng trong stress nước - stress oxy ở cây Arabidopsis dại, thiếu hụt ABA, thiếu hụt JA và thiếu hụt Ascorbate
Tóm tắt
Sự tương tác giữa axit jasmonic (JA) và axit abscisic (ABA) trong phản ứng của cây đối với stress nước và stress oxy gia tăng do stress nước đã được nghiên cứu trên cây Arabidopsis thaliana bị stress nước do thiếu nước. Để thực hiện điều này, một thử nghiệm hạn hán đã được tiến hành sử dụng các biến thể Arabidopsis bị suy giảm trong tổng hợp ABA (aba2), JA (aos), và ascorbate (vtc1). Kết quả của chúng tôi cho thấy có sự tương tác giữa ABA và JA trong quá trình tổng hợp của chúng. Hơn nữa, sự phối hợp hành động của ABA và JA đã bảo vệ các cây kiểu hoang dã, aba2 và aos khỏi tác động của stress. Tuy nhiên, tác dụng này không được quan sát ở biến thể vtc1, mà cho thấy một sự giảm rõ rệt trong tỷ lệ Fv/Fm, đi kèm với sự sụt giảm đáng kể trong hàm lượng nước tương đối (RWC), mặc dù nồng độ nội sinh của JA và ABA rất cao. Phát hiện này chỉ ra sự liên quan của chuyển hóa ascorbate trong việc thích nghi của cây với stress. Mặc dù có sự tương tác giữa hai phytohormone, việc đóng khí khổng do hạn hán chủ yếu được điều tiết bởi ABA và yếu hơn bởi JA, trong khi JA đóng vai trò trong việc hình thành các chất chống oxy hóa điều chỉnh chuyển hóa ascorbate và glutathione. Phân tích theo thời gian đã tiết lộ tầm quan trọng của độ tuổi của cây và thời gian chịu stress trong phản ứng của các biến thể so với các cây kiểu hoang dã. Tại đây, chúng tôi thảo luận về mối quan hệ giữa ABA, JA, ascorbate và glutathione ở các cây chịu stress nước.
Từ khóa
#Axit Abscisic #Axit Jasmonic #Arabidopsis thaliana #stress nước #stress oxy #ascorbate #glutathione #phytohormoneTài liệu tham khảo
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