Interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) cells and their precursors express the VGO1 antigen

Springer Science and Business Media LLC - Tập 9 - Trang 362-367 - 1989
Jorge F. Denegri1, Jeanne Peterson1, Peter Tilley1
1Immunology Laboratory, Division of Hematopathology, Department of Pathology, Vancouver General Hospital and University of British Columbia, Vancouver, Canada

Tóm tắt

Precursor and effector cells of recombinant interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) activity were investigated for their expression of VGO1. Peripheral blood lymphocytes (PBL) from normal donors were purified and separated in a FACS 420 into VGO1+-and VGO1−-cell fractions before and after culture for 96 hr with 100 U/ml of r-IL-2. Their lytic activity against K 562 and Daudi cells was measured in a51Cr release assay. The majority, if not all, of the LAK effector and precursor cells was VGO1+ lymphocytes. The expression of VGO1 by LAK precursor cells remained stable under the culture conditions used in our experiments. VGO1− lymphocytes cultured with r-IL-2 demonstrated neither LAK-induced activity nor expression of VGO1 antigen.

Tài liệu tham khảo

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