Interaction of [125I]β nerve growth factor with acidic proteins
Tóm tắt
We have demonstrated that the β nerve growth factor will interact with various acidic proteins apparently nonspecifically. When125I-labeled β nerve growth factor at a concentration of 3.8×10−10 M is incubated with an acidic protein at 2 mg/ml (4.5×10−6–4.4×10−5 M), a complex is formed. This complex changes the isoelectric point of the125I-labeled β nerve growth factor sufficiently so that the125I-labeled β nerve growth factor migrates anomalously in polyacrylamide gel electrophoresis. The interaction between β nerve growth factor and bovine serum albumin, which appears to be complex, may be the cause of the previously reported activation of the β nerve growth factor when bovine serum albumin is present in a typical bioassay.
Tài liệu tham khảo
Levi-Montalcini, R., andAngeletti, P. 1968. Nerve growth factor. Physiol. Rev. 48:534–569.
Server, A. C., andShooter, E. M. 1977. Pages 339–409in Anfinsen, C. B., Edsall, J. T., andRichards, F. M. (eds.), Advances in Protein Chemistry, Academic Press, New York.
Mobley, W. C., Server, A. C., Ishii, D. N., Riopelle, R. J., andShooter, E. M. 1977. Nerve growth factor. N. Engl. J. Med. 297:1096–1104, 1149–1158, 1211–1218.
Ronne, H., Anundi, H., Rask, L., andPeterson, P. A. 1979. Nerve growth factor binds to serum alpha-2-monoglobulin. Biochem. Biophys. Res. Commun. 87:330–336.
Callisono, P., andCozzari, C. 1974. Interaction of nerve growth factor with the mouse-brain neurotubule protein(s). Proc. Natl. Acad. Sci. U.S.A. 71:2131–2135.
Almon, R. R., andVaron, S. 1978. Association of beta nerve growth factor with bovine serum albumin as well as with the alpha and gamma subunits of the 7S macromolecule. J. Neurochem. 30:1559–1567.
Oleander, E. J., andStach, R. W. 1977. High affinity, specific binding of [125I]beta nerve growth factor to glass beads. Biochem. Biophys. Res. Commun. 79:561–568.
Stach, R. W., Garian, N., andOleander, E. J. 1979. Biological activity of the β nerve growth factor: The effects of various added proteins. J. Neurochem. 33:257–261.
Stach, R. W., Wagner, B. J., andStach, B. M. 1977. A more rapid method for the isolation of the 7S nerve growth factor complex. Anal. Biochem. 83:26–32.
Rice, B. L., andStach, R. W. 1976. The dimer-monomer equilibrium constant for125I-β nerve growth factor. Biochem. Biophys. Res. Commun. 73:479–485.
Oleander, E. J., andStach, R. W. 1980. Sequestration of125I-labelled β nerve growth factor. J. Biol. Chem. 255:9338–9343.
Weber, K., andOsborn, M. 1969. The reliability of molecular weight determinations by dodecylsulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406–4412.
Stach, R. W., andShooter, E. M. 1974. The biological activity of cross-linked beta nerve growth factor protein. J. Biol. Chem. 249:6668–6674.
Malik, N., andBerrie, A. 1972. A new stain fixative for proteins separated by gel isoelectric focussing based on Coomassie brilliant blue. Anal. Biochem. 49:173–176.
Gey, G. O., andGey, M. K. 1936. The maintenance of human normal cells and tumor cells in continuous culture. I. Preliminary report: Cultivation of mesoblastic tumors and normal tissue and notes on methods of cultivation. Am. J. Cancer 27:45–76.
Calissono, P., Monaco, G., Castellani, L., Mercanti, D., andLevi, A. 1978. Nerve growth factor poteintiates actomyosin adenosine triphosphatase. Proc. Natl. Acad. Sci. U.S.A. 75:2210–2214.
Pearce, F. L., Banthorpe, D. V., Cook, J. M., andVernon, C. A. 1973. Adsorption of nerve growth factor onto surfaces. Implications for the assay in tissue culture. Eur. J. Biochem. 32:569–575.
Greene, L. A., Varon, S., Piltch, A., andShooter, E. M. 1971. Substructure of the β subunit of mouse 7S nerve growth factor. Neurobiology 1:37–48.
Herrup, K., andShooter, E. M. 1973. Properties of the beta nerve growth factor receptor of avian dorsal root ganglia. Proc. Natl. Acad. Sci. U.S.A. 70:3884–3888.
Banerjee, S. P., Snyder, S. H., Cuatrecasas, P.,andGreene, L. A. 1973. Binding of the nerve growth factor on synpathetic ganglia. Proc. Natl. Acad. Sci. U.S.A. 70:2519–2523.
Banerjee, S. P., Cuatrecasas, P., andSnyder, S. H. 1975. Nerve growth factor receptor binding: Influence of ions, enzymes, and protein reagents. J. Biol. Chem. 250:1417–1433.
Frazier, W. A., Boyd, L. F., andBradshaw, R. A. 1974. Properties of the specific binding of125I-nerve growth factor to responsive peripheral neurons. J. Biol. Chem. 249:5513–5519.
Frazier, W. A., Boyd, L. F., Pulliam, M. W., Szutowicz, A., andBradshaw, R. A. 1974. Properties and specificity of binding sites for125I-nerve growth factor in embryonic heart and brain. J. Biol. Chem. 249:5918–5923.
Sutter, A., Reopelle, R. J., Harris-Warrick, R. M., andShooter, E. M. 1979. Nerve growth factor receptors. J. Biol. Chem. 254:5972–5982.
Malamud, D., andDrysdale, J. W. 1978. Isoelectric points of proteins: a table. Anal. Biochem. 86:620–647.
Hedrick, J. L., andSmith, A. J. 1968. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Arch. Biochem. Biophys. 126:155–164.