Integration and Potential Application Ability of Culturable Functional Microorganism in Oil Tea Camellia

Springer Science and Business Media LLC - Tập 61 - Trang 1-9 - 2020
Caixia Liu1,2,3, Longsheng Chen1,2, Zhilong He1,2, Zhen Zhang1,2, Yanming Xu1,2, Zhigang Li1,2, Yinghe Peng1,2, Nan Deng1, Yongzhong Chen1,2
1Hunan Academy of Forestry, Changsha, China
2National Engineering Research Center for Oil-tea Camellia, Changsha, China
3Central South University of Forestry and Technology, Changsha, China

Tóm tắt

Oil tea Camellia is a major woody oil plant, which has a positive influence on alleviating the contradiction between supply and demand of edible oil in China. Microbial fertilizer for Oil tea Camellia is urgently needed in current production, and it is of great significance to improve the yield and quality. Culturable functional microorganisms are the basis of research and development for microbial fertilizer. In this study, culturable microorganisms which had abilities of antagonism, growth promotion, phosphorus solubility, nitrogen fixation and drought resistance, were integrated from oil tea literature. And the strains potential application ability were evaluated in terms of functionality, safety and adaptability, culture characteristics, suitable conditions and colonization or infection ability of strains. The results showed that the strains with strongest antagonistic ability were Bacillus amyloliquefaciens D2WM and Bacillus subtilis Y13. Beauveria bassiana BbTK-01 and Metarhizium anisopliae FJMa201101 had the strongest insect resistant ability. Glomus versiforme and Glomus intraradices can promote oil tea fastest growth. Phosphorus dissolving ability of Bacillus aryabhattai NC285 and Burkholderia cepacia 6-Y-09 were strongest. The strains with strongest Nitrogen fixing ability were Azomonas N7-3 and Sphingobium B7-7, and the strains with strongest improving drought resistance ability were Glomus versiforme and Glomus intraradices. Comprehensive evaluation of strains showed that Bacillus subtilis Y13 and Azomonas N7-3 had a good applied potential ability. This study would save time-consuming of isolate, purify and identify repetitively for the researchers of functional bacteria of oil tea Camellia. Meanwhile it provides a research basis for selecting targeted strains and constructing the combination of functional strains, therefore provides data support for fertilizer efficiency.

Tài liệu tham khảo

Chen YZ, Peng SF, Wang XN, Yang XH, He JH, Wang DB (2007) Study of high yield cultivation technologies of oil-tea camellia (Camellia oleifera)–formulate fertilization. Forest Res 20:650–655. https://doi.org/10.3321/j.issn:1001-1498.2007.05.010 Li Y, Huang YF, Ye XP, Liang WM (2013) Effects of intercropping soybean on nutrient contents in soil at Camellia oleifera young forest. Nonwood Forest Res 31:54–59. https://doi.org/10.14067/j.cnki.1003-8981.2013.02.005 Zheng H, Ouyang ZY, Wang XK, Fang ZG, Zhao TQ, Miao H (2005) Effects of regenerating forest cover on soil microbial communities: A case study in hilly red soil region, Southern China. Forest Ecol Manag 217: 244-254. https://doi.org/10.1016/jforeco.2005.06.005 Liu CX, Chen LS, Wei T, Peng SF, Li MQ, Deng N, Chen YZ (2018) Predicting potential distribution and evaluating suitable soil condition of oil tea camellia in China. Forests 9:487. https://doi.org/10.3390/f9080487 Jansson JK (2013) Microbiology: the life beneath our feet. Nature 494:40–41. https://doi.org/10.1038/494040a Hacquard S, Garrido-Oter R, González A, Spaepen S, Ackermann G, Lebeis S, McHardy AC, Dang GL, Knight R, Ley R, Schulze-Lefert P (2015) Microbiota and host nutrition across plant and animal kingdoms. Cell Host Microbe 17:603–616. https://doi.org/10.1016/j.chom.2015.04.009 Song CQ, Wu JS, Lu YH, Shen QR, He JZ, Huang QY, Jia ZJ, Leng SY, Zhu YG (2013) Advances of soil microbiology in the last decade in China. Adv Earth Sci 28:1087–1105. https://doi.org/10.11867/j.issn.1001-8166.2013.10.1087 Vilanova C, Porcar M (2016) Are multi-omics enough? Nat Microbiol. https://doi.org/10.1038/nmicrobiol.2016.101 Fan NS, Qi R, Yang M (2016) Current technical progresses in the cultivation for uncultured microorganism. China J Appl Environ Biol 3:524–530. https://doi.org/10.3724/SP.J.1145.2015.09023 Liu CX, Jiao RZ, Dong YH, Sun QW, Zhou XH, Li FQ (2015) Response of the N-cycling associated soil microorganism to simulated N deposition in a plantation of Cunninghamia lanceolata. Scientia Silvae Sinicae 51:96–102. https://doi.org/10.11707/j.1001-7488.20150412 Newman DK, JF Banfield (2002) Geomicrobiology: how molecular-scale interactions underpin biogeochemical systems. Science 296:1071–1077. https://doi.org/10.1126/science.1010716 Bai Y, Müller DB, Srinivas G, Garrido-Oter R, Potthoff E, Rott M, Dombrowski N, Münch PC, Spaepen S, Remus-Emsermann M, Hüttel B, McHardy AC, Vorholt JA, Schulze-Lefert P (2015) Functional overlap of the Arabidopsis leaf and root microbiota. Nature 528:364–369. https://doi.org/10.1038/nature16192 Wei M, Zhang W, Guan WX, Zhu JQ, Fu BZ, Wang LH, Li GY (2017) Screening and identification of an antagonistic strain against fungal diseases in Camellia oleifera and its antagonistic effect. Jiangsu Agr Sci 45:100–104. https://doi.org/10.15889/j.issn.1002-1302.2017.18.026 Zhou GY, Chen Y, Liu AJ, Dong XN, Song GT, Gou ZH (2010) Resistance to anthracnose in Camellia oleifera induced by antagonistic bacteria. Forest Pest Disease 29:1–3. https://doi.org/10.3969/j.issn.1671-0886.2010.03.001 Bu TT, GY Zhou, Liu JA, He Li (2012) Analysis of 16S rDNA sequence and bacteriostatic mechanism of endophytic antagonistic bacteria Y13 in Camellia oleifera. Nonwood Forest Res 30: 11–15. https://doi.org/10.14067/j.cnki.1003-8981.2012.03.004 Jin Q, Zhu DX, Zhou GY, Li H, He YH, Zhang Q (2017) Colonization of GFP-Tagged Bacillus subtilis Y13UV in Camellia oleifera. Scientia Silvae Sinicae 53:111–117. https://doi.org/10.11707/j.1001-7488.201707012 Xin SS, Qi GF, Zhu FY, Lu JZ, Li JJ, Wang SY, Liu YJ, Chen JY, Wang YX, Wang XY (2011) Optimization of fermentation condition for Bacillus amyloliquefaciens WH1 and its biological control effect on Colletotrichum gloeosporioides. J Huazhong Agric Univ 30:411–415 Wei M, Lu L, Li CQ, Fu BZ, Li GY, Wang LH (2016) Identification of antagonistic fungus to camellia diseases and study of biological characteristics and antagonism effect. J Henan Agri Sci 45:74–80. https://doi.org/10.15933/j.cnki.1004-3268.2016.08.014 Song GT, Zhou GY, Liu JA, Li H, Li L (2010) Isolation and characterization of antagonistic endophytic bacteria again Fusarium proliferatum. Acta Phytophylacica Sinica 37:137–142. https://doi.org/10.1016/0379-6779(92)90102-O Hu F, Hu BJ, Li CC, Zhou ZY, Xu LN, Li RX (2015) Preliminary study on Paenibacillus polymyxa DN-1 against camellia anthracnose. Forest Pest Disease 34:1–5. https://doi.org/10.3969/j.issn.1671-0886.2015.04.001 Wu ZL (2016) Study on soil microbial diversity of Camellia oleifera. Cent South Univ Fore Technol 45–55 Lu DS, Chan S, Dai B (2012) Selection of antagonistic fungi to species of pathogens on Camellia oleifera. J Xinyang Normal Uni (Natural Sci Edition) 25:182–185. https://doi.org/10.3969/j.issn.1003-0972.2012.02.011 Zhou GY, Gou ZH, Hao Y, Li H (2010) Screening antagonistic silicate bacteria from Camellia oleifera rhizosphere against Fusarium proliferatum and stability of bacteria. J Cent South Univ For Technol 30:118–122. https://doi.org/10.3724/SP.J.1011.2010.01267 Li XJ (2011) Isolation, screening and development of biocontrol agent of endophytic antagonistic bacteria to Camellia soft rot disease. Cent South Univ For Technol 1–2 Song GT, Zhou GY (2012) Study on inhibitory mechanism of antagonistic streptomyces F10 to Colletotrichum gloeosporioide. J Cent South Univ of Fore Technol 32:90–93. https://doi.org/10.14067/j.cnki.1673-923x.2012.08.021 Ni F (2015) The acquisition of Camellia pests data and database construction. Hunan Agric Uni 2–5 Davari B, Limoee M, Khodavaisy S, Zamini G, Izadi S (2015) Toxicity of entomopathogenic fungi, Beauveria bassiana and Lecanicillium muscarium against a field-collected strain of the German cockroach Blattella germanica (L.) (Dictyoptera: Blattellidae). Trop Biomed 32:463–470 Yasin M, Wakil W, Ghazanfar MU, Qayyum MA (2017) Virulence of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against red palm weevil, Rhynchophorus ferrugineus (Olivier). Entomol Res 49:1–10. https://doi.org/10.1111/1748-5967.12260 Behie SW, Jones SJ, Bidochka MJ (2015) Plant tissue localization of the endophytic insect pathogenic fungi Metarhizium and Beauveria. Fungal Ecol 13:112–119. https://doi.org/10.1016/j.funeco.2014.08.001 He XY, Cai SP, Tong YH, Xiong Y, Huang Y, Xie JD, Chen SL (2011) ) Pathogenicity evaluation of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against adults of Basilepta melanopus (Coleoptera:Eumolpidae). Acta Entomol Sin 54:1281–1287. https://doi.org/10.1017/S0022112010006427 He XY, Cai SP, Du YF, Chen DL, Huang JS, Li KQ (2015) Screening of Metarhizium anisopliae strain with high virulence against larvae of Curculio chinensis (Coleoptera: Curculionidae). Scientia Silvae Sinicae 51:52–59. https://doi.org/10.11707/j.1001-7488.20150807 Deng XJ (2012) The mutation breeding by N+ implantation in Beauveria bassiana and detection on the virulence against pests of Camellia oleifera. Cent South Univ For Technol 4–7 Lin XB (2008) Screening of Beauveria bassiana strains with high toxicity to Enproctis pseudoconspersa. Wuyi Sci J 24:78–81. https://doi.org/10.15914/j.cnki.wykx.2008.00.015 Wang DX, Zhang NY, Chen GC (2011) Effects of AM Fungi on the Growth and Drought-resistance of Camellia oleifera. Guangxi Fore Sci 40:259–261. https://doi.org/10.19692/j.cnki.gfs.2011.04.004 Xiang WL, Feng W, Guo JH, Song P, Tang K, Yang ZR (2009) Identification of a moderate halophilic phosphate—dissolving bacterium and its phosphate-solubility. Microbiology China 36:320–327. https://doi.org/10.13344/j.microbiol.china.2009.03.019 Park JH, Bolan N, Megharaj M, Naidu R (2011) Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. J Hazard Mater 185:829–836. https://doi.org/10.1016/j.jhazmat.2010.09.095 Hao Y (2009) Study on soil microorganisms and enzymatic activity in oiltea Camellia stands and screen of high efficiency phosphate-solublizing strain. Cent South Univ For Technol 57–61 Liu XY, Fu DQ, Chen LQ, Yang WB, Li DX, Fu HQ (2015) Screening and identification of phosphate solubilizing bacteria in the rhizosphere of camellia. Guangdong Agr Sci 42:47–50. https://doi.org/10.16768/j.issn.1004-874x.2015.03.008 Liu XY, Fu DQ, Chen LQ, Yang WB, Li DX, Fu HQ, Jia XC (2015) Isolation, identification and phosphate-solubilizing capacity of phosphate-solubilizing bacteria from the rhizosphere of camellia. Biotechnol bull 31:169–173. https://doi.org/10.13560/j.cnki.biotech.bull.1985.2015.07.025 Wang S, Zhang LP, Hao FF, Zhang Y (2015) Screening, identification and security test of Camellia oleifera rhizosphere phosphate-solubilizing bacteria. For Res 28:166–172. https://doi.org/10.13275/j.cnki.lykxyj.2015.02.005 Wang S, Zhang LP, Zhang Y, Hao FF, Xiao JX (2015) Screening, identification and phosphate solubilizing capability of phosphate solubilizing bacteria in rhizosphere of Camellia oleifera Abel at red soil region. For Res 28:409–416. https://doi.org/10.3969/j.issn.1001-1498.2015.03.016 Liu XY, Fu DQ, Jia XC, Chen LQ (2016) Isolation, identification and culture condition of phosphate-solubilizing bacteria derived from camellia rhizosphere soil. Southwest China J Agr Sci 29:2637–2642. https://doi.org/10.16213/j.cnki.scjas.2016.11.023 Chen XY (2011) Study on associative nitrogen fixation rhizobacterial strains of Woody Plants: a case study of Camellia oleifera, Cunninghamia lanceolata and Poplar. Cent South For Coll 19–27 Gou ZH (2010) The combinatorial optimization and fertilizer efficiency of the oiltea Camellia rhizobacterial strains. Cent South Univ For Technol 22–41 Cai M (2011) Isolation and characteristics of associative nitrogen fixation bacteria in different kinds of stand of Camellia olefera. Nanjing For Univ 4–12 Peng FR, Liang YW, Cai M, Chen LS, Chen YZ (2014) The 16SrDNA sequences analysis of associative nitrogen fixing bacteria from rhizosphere of Camellia oleifera. J Nanjing Fore Uni (Natural Sci) 38:27–30. https://doi.org/10.3969/j.issn.1000-2006.2014.01.005 Jayne B, Quigley M (2014) Influence of arbuscular mycorrhiza on growth and reproductive response of plants under water deficit:a meta-analysi. Mycorrhiza 24:109–119. https://doi.org/10.1007/s00572-013-0515-x Xu JG, Sun T, Li S (2016) Application of microbial fertilizers in agricultural production of China. Crops 1:1–6. https://doi.org/10.16035/j.issn.1001-7283.2016.01.001 Kohler J, Hernández JA, Caravaca F, A Roldan (2008) Plant-growth-promoting rhizobacteria and arbuscular mycorrhizal fungi modify alleviation biochemical mechanisms in water-stressed plants. Funct Plant Biol 35:141–151. https://doi.org/10.1071/FP07218 Strassmann JE, Gilbert OM, Queller DC (2011) Kin discrimination and cooperation in microbes. Annu Rev Microbiol 65:349–367. https://doi.org/10.1146/annurev.micro.112408.134109 Etesami H, Maheshwari DK (2018) Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicol Environ Saf 156:225–246. https://doi.org/10.1016/j.ecoenv.2018.03.013 Wu TL, Chen Y, Ma L, Zeng LB, Li LD (2016) Isolation and identification of an antagonistic bacteria to Colletotrichum gloeosporioides of Camellia oleifera. Hunan For Sci Technol 43:62–66. https://doi.org/10.3969/j.issn.1003-5710.2016.05.012 Benizri E, Baudoin E, Guckert A (2001) Root colonization by inoculated plant growth-promoting rhizobacteria. Biocontrol Sci Techn 11:557–574. https://doi.org/10.1080/09583150120076120 Alabouvette C, Olivain C, Steinberg C (2006) Biological control of plant diseases: The European Situation. Eur J Plant Pathol 114:329–341. https://doi.org/10.1007/s10658-005-0233-0 Griffin AS, West SA, Buckling A (2004) Cooperation and competition in pathogenic bacteria. Nature 430:1024–1027. https://doi.org/10.1038/nature02744 West SA, Griffin AS, Gardner A (2006) Social evolution theory for microorganisms. Nat Rev Microbiol 4:597–607. https://doi.org/10.1038/nrmicro1461 Mulvaney RL, Khan SA, Ellsworth TR (2009) Synthetic nitrogen fertilizers deplete soil nitrogen: a global dilemma for sustainable cereal production. J Environ Qual 38:2295. https://doi.org/10.2134/jeq2008.0527 Itelima JU, Bang WJ, Onyimba IA, Sila MD, Egbere OJ (2018) Biofertilizer: a key player in enhancing soil fertility and crop productivity—a review. Direct Res J Agric Food Sci 2:22–28. https://doi.org/10.26765/DRJAFS.2018.4815 Ma MC, Jiang X, Wang QF, Guan D, Li L, Ongena M, Li J (2018) Isolation and identification of PGPR strain and its effect on soybean growth and soil bacterial community composition. Int J Agric Biol 20:1289–1297. https://doi.org/10.17957/IJAB/15.0627 Shen DL, Li J, Jiang X (2013) Status quo and development of microbial fertilizer industry in China. J Microbiol 33:1–4. https://doi.org/10.3969/j.issn.1005-7021.2013.03.001