Inhibitory effect of Acanthopanax extract on the dopaminergic neuroblastoma cells against rotenone-induced toxicity
Tóm tắt
Abstract During screening of Korean indigenous medicinal herbs with anti-neurodegenerative activities, we found that the extract of Acanthopanax divaricatus vat. albeofructus could inhibit rotenone-induced DNA and cell damage in neuroblastoma cells. A simple in vitro model developed for the study of possible mechanisms underlying neurodegeneration in Parkinson’s disease includes the administration of rotenone to the human dopaminergic neuroblastoma cell line, SH-SY5Y. In this investigation, rotenone induced oxidative DNA damage of lymphocytes as expected. However, the oxidative DNA damage was inhibited in vitro upon treating Acanthopanax extract. Moreover, Acanthopanax extract resulted in the inhibitory activities against cell damage, ROS generation and chromatin condensation by rotenone.
Tài liệu tham khảo
Farooqui, T. & Farooqui, A. A. Lipid-mediated oxidative stress and inflammation in the pathogenesis of Parkinson’s disease. Parkinsons Dis. 2011, 247467 (2011).
Watabe, M. & Nakaki, T. Mitochondrial complex I inhibitor rotenone-elicited dopamine redistribution from vesicles to cytosol in human dopaminergic SH-SY5Y cells. J. Pharmacol. Exp. Ther. 323, 499–507 (2007).
Cabeza-Arvelaiz, Y. & Schiestl, R. H. Transcriptome analysis of a rotenone model of parkinsonism reveals complex I-tied and -untied toxicity mechanisms common to neurodegenerative diseases. PLoS One 7, e44700 (2012).
Uttara, B., Singh, A. V., Zamboni, P. & Mahajan, R. T. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr. Neuropharmacol. 7, 65–74 (2009).
Sutachan, J. J. et al. Cellular and molecular mechanisms of antioxidants in Parkinson’s disease. Nutr. Neurosci. 15, 120–126 (2012).
Kim, K. J., Hong, H. D., Lee, O. H. & Lee, B. Y. The effects of Acanthopanax senticosus on global hepatic gene expression in rats subjected to heat environmental stress. Toxicology 278, 217–223 (2010).
Kang, O. H., Kim, D. K., Cai, X. F., Kim, Y. H. & Lee, Y. M. Attenuation of experimental murine colitis by acanthoic acid from Acanthopanax koreanum. Arch. Pharm. Res. 33, 87–93 (2010).
Wu, Y. L. et al. Acanthoic acid, a diterpene in Acanthopanax koreanum, protects acetaminophen-induced hepatic toxicity in mice. Phytomedicine 17, 475–479 (2010).
Park, S. K., Jeon, Y. M., Son, B. S., Youn, H. S. & Lee, M. Y. Proteomic analysis of the differentially expressed proteins by airborne nanoparticles. J. Appl. Toxicol. 31, 463–470 (2011).
Ribas-Maynou, J. et al. Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor. PLoS One 7, e44679 (2012).
Ki, Y. W., Lee, J. E., Park, J. H., Shin, I. C. & Koh, H. C. Reactive oxygen species and mitogen-activated protein kinase induce apoptotic death of SH-SY5Y cells in response to fipronil. Toxicol. Lett. 211, 18–28 (2012).
Rautenbach, F., Faber, M., Laurie, S. & Laurie, R. Antioxidant capacity and antioxidant content in roots of 4 sweetpotato varieties. J. Food Sci. 75, C400–C405 (2010).
Lin, T. K. et al. Mitochondrial dysfunction and oxidative stress promote apoptotic cell death in the striatum via cytochrome c/caspase-3 signaling cascade following chronic rotenone intoxication in rats. Int. J. Mol. Sci. 13, 8722–8739 (2012).
Rojo, A. I., Cavada, C., de Sagarra, M. R. & Cuadrado, A. Chronic inhalation of rotenone or paraquat does not induce Parkinson’s disease symptoms in mice or rats. Exp. Neurol. 208, 120–126 (2007).
Maiese, K., Chong, Z. Z., Hou, J. & Shang, Y. C. Oxidative stress: Biomarkers and novel therapeutic pathways. Exp. Gerontol. 45, 217–234 (2010).
Bermejo-Bescós, P., Pi~nero-Estrada, E. & Villar del Fresno, A. M. Neuroprotection by Spirulina platensis protean extract and phycocyanin against iron-induced toxicity in SH-SY5Y neuroblastoma cells. Toxicol. In Vitro 22, 1496–1502 (2008).
Nhiem, N. X. et al. Lupane triterpene glycosides from leave of Acanthopanax koreanum and their cytotoxic activity. Chem. Pharm. Bull. 57, 986–989 (2009).
Singh, N. P., McCoy, M. T., Tice, R. R. & Schneider, E. L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184–191 (1988).
Toyoizumi, T. et al. Evaluation of effect during cell isolation process in alkaline comet assay using epidermal skin cells. J. Toxicol. Sci. 37, 1267–1273 (2012).
Kwon, S. H. et al. Loganin protects against hydrogen peroxide-induced apoptosis by inhibiting phosphorylation of JNK, p38, and ERK 1/2 MAPKs in SH-SY5Y cells. Neurochem. Int. 58, 533–541 (2011).