Inhibition of release of inflammatory mediators in primary and cultured cells by a Chinese herbal medicine formula for allergic rhinitis
Tóm tắt
We demonstrated that a Chinese herbal formula, which we refer to as RCM-101, developed from a traditional Chinese medicine formula, reduced nasal and non-nasal symptoms of seasonal allergic rhinitis (SAR). The present study in primary and cultured cells was undertaken to investigate the effects of RCM-101 on the production/release of inflammatory mediators known to be involved in SAR. Compound 48/80-induced histamine release was studied in rat peritoneal mast cells. Production of leukotriene B4 induced by the calcium ionophore A23187 was studied in porcine neutrophils using an HPLC assay and lipopolysaccharide-stimulated prostaglandin E2 production was studied in murine macrophage (Raw 264.7) cells by immune-enzyme assay. Expression of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) was determined in Raw 264.7 cells, using western blotting techniques. RCM-101 (1–100 μg/mL) produced concentration-dependent inhibition of compound 48/80-induced histamine release from rat peritoneal mast cells and of lipopolysaccharide-stimulated prostaglandin E2 release from Raw 264.7 cells. Over the range 1 – 10 μg/mL, it inhibited A23187-induced leukotriene B4 production in porcine neutrophils. In addition, RCM-101 (100 μg/mL) inhibited the expression of COX-2 protein but did not affect that of COX-1. The findings indicate that RCM-101 inhibits the release and/or synthesis of histamine, leukotriene B4 and prostaglandin E2 in cultured cells. These interactions of RCM-101 with multiple inflammatory mediators are likely to be related to its ability to reduce symptoms of allergic rhinitis.
Tài liệu tham khảo
Bousquet J, Vignola AM, Demoly P: Links between rhinitis and asthma. Allergy. 2003, 58 (8): 691-706. 10.1034/j.1398-9995.2003.00105.x.
Bachert C, Bousquet J, Canonica GW, Durham SR, Klimek L, Mullol J, Van Cauwenberge PB, Van Hammee G: Levocetirizine improves quality of life and reduces costs in long-term management of persistent allergic rhinitis. J Allergy Clin Immunol. 2004, 114 (4): 838-844. 10.1016/j.jaci.2004.05.070.
Senti G, Johansen P, Martinez Gomez J, Prinz Varicka BM, Kundig TM: Efficacy and safety of allergen-specific immunotherapy in rhinitis, rhinoconjunctivitis, and bee/wasp venom allergies. Int Rev Immunol. 2005, 24 (5-6): 519-531. 10.1080/08830180500370944.
Australian Bureau of Statistics: National Health Survey: Summary of Results, 2004-05, Cat no 4364.0. 2006, Canberra , ABS
Baraniuk JN: Pathogenesis of allergic rhinitis. J Allergy Clin Immunol. 1997, 99 (2): S763-72. 10.1016/S0091-6749(97)70125-8.
Davies RJ, Bagnall AC, McCabe RN, Calderon MA, Wang JH: Antihistamines: topical vs oral administration. Clin Exp Allergy. 1996, 26 (Suppl 3): 11-17. 10.1111/j.1365-2222.1996.tb00653.x.
Salib RJ, Howarth PH: Safety and tolerability profiles of intranasal antihistamines and intranasal corticosteroids in the treatment of allergic rhinitis. Drug Saf. 2003, 26 (12): 863-893. 10.2165/00002018-200326120-00003.
Passalacqua G, Bousquet PJ, Carlsen KH, Kemp J, Lockey RF, Niggemann B, Pawankar R, Price D, Bousquet J: ARIA update: I--Systematic review of complementary and alternative medicine for rhinitis and asthma. J Allergy Clin Immunol. 2006, 117 (5): 1054-1062. 10.1016/j.jaci.2005.12.1308.
Xue CC, Thien FC, Zhang JJ, Da Costa C, Li CG: Treatment for seasonal allergic rhinitis by Chinese herbal medicine: a randomized placebo controlled trial. Altern Ther Health Med. 2003, 9 (5): 80-87.
Lenon GB, Li CG, Xue CCL, Thien FCK, Story DF: Inhibition of Release of Vasoactive and Inflammatory Mediators in Airway and Vascular Tissues and Macrophages By a Chinese Herbal Medicine Formula for Allergic Rhinitis . Evid Based Complement Alternat Med. 2006, doi: 10.1093/ecam/ne1083
Ikarashi Y, Yuzurihara M, Sakakibara I, Takahashi A, Ishimaru H, Maruyama Y: Effects of an oriental herbal medicine, "Saiboku-to", and its constituent herbs on Compound 48/80-induced histamine release from peritoneal mast cells in rats. Phytomedicine. 2001, 8 (1): 8-15. 10.1078/0944-7113-00012.
Provost P, Borgeat P, Merhi Y: Platelets, neutrophils, and vasoconstriction after arterial injury by angioplasty in pigs: effects of MK-886, a leukotriene biosynthesis inhibitor. Br J Pharmacol. 1998, 123 (2): 251-258. 10.1038/sj.bjp.0701611.
Hwang BY, Lee JH, Koo TH, Kim HS, Hong YS, Ro JS, Lee KS, Lee JJ: Furanoligularenone, an Eremophilane from Ligularia fischeri, Inhibits the LPS-Induced Production of Nitric Oxide and Prostaglandin E2 in Macrophage RAW264.7 Cells. Planta Med. 2002, 68 (2): 101-105. 10.1055/s-2002-20250.
Shin KM, Kim IT, Park YM, Ha J, Choi JW, Park HJ, Lee YS, Lee KT: Anti-inflammatory effect of caffeic acid methyl ester and its mode of action through the inhibition of prostaglandin E2, nitric oxide and tumor necrosis factor-alpha production. Biochem Pharmacol. 2004, 68 (12): 2327-2336. 10.1016/j.bcp.2004.08.002.
Betts WH, Hurst NP, Murphy GA, Cleland LG: Auranofin stimulates LTA hydrolase and inhibits 5-lipoxygenase/LTA synthase activity of isolated human neutrophils. Biochem Pharmacol. 1990, 39 (7): 1233-1237. 10.1016/0006-2952(90)90268-P.
Seo WG, Pae HO, Oh GS, Chai KY, Kwon TO, Yun YG, Kim NY, Chung HT: Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide productions by murine macrophage cell line, RAW 264.7 cells. J Ethnopharmacol. 2001, 76 (1): 59-64. 10.1016/S0378-8741(01)00221-5.
Naclerio RM: Allergic rhinitis. N Engl J Med. 1991, 325 (12): 860-869.
Kuby J: Immunology. 1994, New York , W.H. Freemen and company, 2nd
Kim SJ, Kim MS: Inhibitory effects of cimicifugae rhizoma extracts on histamine, bradykinin and COX-2 mediated inflammatory actions. Phytother Res. 2000, 14 (8): 596-600. 10.1002/1099-1573(200012)14:8<596::AID-PTR731>3.0.CO;2-V.
Kim HM, Yi JM, Lim KS: Magnoliae flos inhibits mast cell-dependent immediate-type allergic reactions. Pharmacol Res. 1999, 39 (2): 107-111. 10.1006/phrs.1998.0414.
Fung D, Lau CB: Schizonepeta tenuifolia: chemistry, pharmacology, and clinical applications. J Clin Pharmacol. 2002, 42 (1): 30-36. 10.1177/0091270002042001003.
Lee YM, Hirota S, Jippo-Kanemoto T, Kim HR, Shin TY, Yeom Y, Lee KK, Kitamura Y, Nomura S, Kim HM: Inhibition of histamine synthesis by glycyrrhetinic acid in mast cells cocultured with Swiss 3T3 fibroblasts. Int Arch Allergy Immunol. 1996, 110 (3): 272-277.
Ishiguro K, Oku H, Suitani A, Yamamoto Y: Effects of conjugated linoleic acid on anaphylaxis and allergic pruritus. Biol Pharm Bull. 2002, 25 (12): 1655-1657. 10.1248/bpb.25.1655.
Azevedo M, Castel-Branco MG, Oliveira JF, Ramos E, Delgado L, Almeida J: Double-blind comparison of levocabastine eye drops with sodium cromoglycate and placebo in the treatment of seasonal allergic conjunctivitis. Clin Exp Allergy. 1991, 21 (6): 689-694. 10.1111/j.1365-2222.1991.tb03197.x.
Bousquet J, Vignola AM, Campbell AM, Michel FB: Pathophysiology of allergic rhinitis. Int Arch Allergy Immunol. 1996, 110 (3): 207-218.
Hamasaki Y, Kobayashi I, Hayasaki R, Zaitu M, Muro E, Yamamoto S, Ichimaru T, Miyazaki S: The Chinese herbal medicine, shinpi-to, inhibits IgE-mediated leukotriene synthesis in rat basophilic leukemia-2H3 cells. J Ethnopharmacol. 1997, 56 (2): 123-131. 10.1016/S0378-8741(97)01520-1.
Zhou J, Yan X, Xie G: Traditional Chinese Medicine: Molecular Structures, Natural Sources and Application. Edited by: Milne GWA. 2003, Hampshire , Ashgate
Koshihara Y, Neichi T, Murota S, Lao A, Fujimoto Y, Tatsuno T: Caffeic acid is a selective inhibitor for leukotriene biosynthesis. Biochim Biophys Acta. 1984, 792 (1): 92-97.
Inoue H, Saito H, Koshihara Y, Murota S: Inhibitory effect of glycyrrhetinic acid derivatives on lipoxygenase and prostaglandin synthetase. Chem Pharm Bull (Tokyo). 1986, 34 (2): 897-901.
Inoue H, Mori T, Shibata S, Koshihara Y: Inhibitory effect of glycyrrhetinic acid derivatives on arachidonic acid-induced mouse ear oedema. J Pharm Pharmacol. 1988, 40 (4): 272-277.