Influence of the application of enemas with oily extract of curcumin (Curcuma longa) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream

Journal of Coloproctology - Tập 37 - Trang 312-319 - 2017
Carlos Augusto Real Martinez1,2, Vitor Piquera de Oliveira1, Fabio Guilherme Campos3, Danilo Toshio Kanno1, Oscar Orlando Araya Fernandez1, Caled Jaoudat Kadri1, José Aires Pereira4
1Universidade São Francisco (USF), Programa de Pós-Graduação em Ciências da Saúde, Bragança Paulista, SP, Brazil
2Universidade Estadual de Campinas (UNICAMP), Departamento de Cirurgia, Campinas, SP, Brazil
3Universidade de São Paulo (USP), Faculdade de Medicina, São Paulo, SP, Brazil
4Universidade São Francisco (USF), Disciplina de Patologia, Bragança Paulista, SP, Brazil

Tóm tắt

AbstractPurpose To verify if the application of enemas containing oily extracts of curcumin preserves the tissue content of mucins in the glands of the colonic mucosa without fecal stream.Method Thirty-six Wistar rats were submitted to diversion of the fecal stream by proximal colostomy and distal mucous fistula. The animals were subdivided into three groups, and accordingly received enemas with saline and oily extract of curcumin at concentrations of 50 mg/kg/day or 200 mg/kg/day. After two or four weeks of intervention, the irrigated colic segments were removed. Neutral and acidic mucins were identified by Periodic-acid Schiff and Alcian-Blue techniques, respectively. The content of both mucin subtypes was measured by computerized morphometry. Mann–Whitney test was used to analyze the results, adopting a significance level of 5% (p ≤ 0.05).Results There was an increase in the tissue content of neutral mucins in animals treated with curcumin at a concentration of 50 mg/kg/day for four weeks, whereas in the group treated with 200 mg/kg/day there was an increase independent of the time of intervention. The content of acidic mucins increased in animals treated with 200 mg/kg/day regardless of the intervention time, whereas in those treated with 50 mg/kg/day an increase was observed only after four weeks.Conclusion Enemas with curcumin preserve the content of neutral and acidic mucins in the colonic epithelium without fecal stream.

Tài liệu tham khảo

Nonose, 2009, Tissue quantification of neutral and acid mucins in the mucosa of the colon with and without fecal stream in rats, Acta Cir Bras, 24, 267, 10.1590/S0102-86502009000400005 Gaudier, 2006, Physiological role of mucins in the colonic barrier integrity, Gastroenterol Clin Biol, 30, 965, 10.1016/S0399-8320(06)73358-9 Mello, 2012, Evaluation of the number of goblet cells in crypts of the colonic mucosa with and without fecal transit, Rev Col Bras Cir, 39, 139, 10.1590/S0100-69912012000200010 Martinez, 2010, Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats, Acta Cir Bras, 25, 231, 10.1590/S0102-86502010000300004 Keli, 1997, Diversion-related experimental colitis in rats, Dis Colon Rectum, 40, 222, 10.1007/BF02054992 Deplancke, 2001, Microbial modulation of innate defense: goblet-cells and the intestinal mucus layer, Am J Clin Nutr, 73, 1131S, 10.1093/ajcn/73.6.1131S Willemsen, 2003, Short chain fatty acids stimulate epithelial mucin 2 expression through differential effects on prostaglandin E1 and E2 production by intestinal myofibroblasts, Gut, 52, 1442, 10.1136/gut.52.10.1442 Gaudier, 2009, Butyrate enemas upregulate MUC genes expression but decrease adherent mucus thickness in mice colon, Physiol Res, 58, 111, 10.33549/physiolres.931271 Johansson, 2013, The gastrointestinal mucus system in health and disease, Nat Rev Gastroenterol Hepatol, 10, 352, 10.1038/nrgastro.2013.35 Martinez, 2010, The importance of oxygen free radicals in the etiopathogenesis of diversion colitis in rats, Acta Cir Bras, 25, 387, 10.1590/S0102-86502010000500002 Pereira, 2013, Evaluation of sucralfate enema in experimental diversion colitis, J Coloproctol (Rio J), 33, 182, 10.1016/j.jcol.2013.08.005 Martinez, 2015, Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis, J Coloproctol, 35, 90, 10.1016/j.jcol.2015.02.007 Martinez, 2013, Enemas with n-acetylcysteine can reduce the level of oxidative damage in cells of the colonic mucosa diverted from the faecal stream, Dig Dis Sci, 58, 3452, 10.1007/s10620-013-2768-9 Caltabiano, 2011, 5-Aminosalicylic acid (5-ASA) can reduce levels of oxidative DNA damage in cells of colonic mucosa with and without fecal stream, Dig Dis Sci, 56, 1037, 10.1007/s10620-010-1378-z Aggarwal, 2013, Curcumin-free turmeric exhibits anti-inflammatory and anticancer activities: identification of novel components of turmeric, Mol Nutr Food Res, 57, 1529, 10.1002/mnfr.201200838 Samarghandian, 2017, Anti-oxidative effects of curcumin on immobilization-induced oxidative stress in rat brain, liver and kidney, Biomed Pharmacother, 87, 223, 10.1016/j.biopha.2016.12.105 Lang, 2015, Curcumin in combination with mesalamine induces remission in patients with mild-to-moderate ulcerative colitis in a randomized controlled trial, Clin Gastroenterol Hepatol, 13, 1444, 10.1016/j.cgh.2015.02.019 Kadri, 2017, Anti-inflammatory effects of enemas containing an oily extract of curcumin in an experimental model of diversion colitis, Histol Histopathol, 32, 161 Martinez, 2017, Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa, Acta Cir Bras, 32, 65, 10.1590/s0102-865020170108 Alves, 2017, Tissue sulfomucin and sialomucin content in colon mucosa without intestinal transit subjected to intervention with Curcuma longa (curcumin), Acta Cir Bras, 32, 182, 10.1590/s0102-865020170030000002 Henningsson, 2002, Combinations of indigestible carbohydrates affect short-chain fatty acid formation in the hindgut of rats, J Nutr, 132, 3098, 10.1093/jn/131.10.3098 den Besten, 2013, The role of short-chain fatty acids in the interplay between diet, gut, microbiota, and host energy metabolism, J Lipid Res, 54, 2325, 10.1194/jlr.R036012 Hu, 2016, Short-chain fatty acids in control of energy metabolism, Crit Rev Food Sci Nutr, 27, 1, 10.1080/10408398.2016.1245650 Tan, 2014, The role of short-chain fatty acids in health and disease, Adv Immunol, 121, 91, 10.1016/B978-0-12-800100-4.00003-9 Agarwal, 1989, Diversion colitis: a nutritional deficiency syndrome?, Nutr Rev, 47, 257, 10.1111/j.1753-4887.1989.tb02857.x Hamer, 2009, Butyrate modulates oxidative stress in the colonic mucosa of healthy humans, Clin Nutr, 28, 88, 10.1016/j.clnu.2008.11.002 Martinez, 2012, Oxidative stress and changes in the content and pattern of tissue expression of β-catenin protein in diversion colitis, J Coloproctol, 32, 343, 10.1590/S2237-93632012000400001 Chaim, 2014, Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis, Acta Cir Bras, 29, 544, 10.1590/S0102-8650201400150001 Bonassa, 2015, Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate, Acta Cir Bras, 30, 328, 10.1590/S0102-865020150050000004 Fernandez, 2017, Evaluation of enemas containing sucralfate in tissue content of MUC-2 protein in experimental model of diversion colitis, ABCD Arq Bras Cir Dig, 30, 132, 10.1590/0102-6720201700020012
Thông tin
Thông tin xuất bản

Journal of Coloproctology

Tập 37

312-319

Thông tin tác giả