Influence of light-emitting diodes on phenylpropanoid biosynthetic gene expression and phenylpropanoid accumulation in Agastache rugosa

Applied Biological Chemistry - 2020
Woo-Tae Park1, Sun Kyung Yeo1, Ramaraj Sathasivam1, Jong‐Seok Park2, Jae Kwang Kim3, Sang Un Park1
1Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea
2Department of Horticultural Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea
3Division of Life Sciences and Bio-Resource and Environmental Center, Incheon National University, Incheon, 22012, South Korea

Tóm tắt

Abstract

Agsatache rugosa (Korean mint), belongs to the mint family and it has various medicinal properties. In addition, it has several valuable compounds such as monoterpenes and phenylpropanoid compounds. Amongst these, two compounds viz., rosmarinic acid (RA), and tilianin are well-known natural compounds that have numerous pharmacological properties. The phenylpropanoid biosynthetic gene expression under stress conditions and the subsequent accumulation of phenylpropanoid content has not been extensively studied in Korean mint. Here, we investigated the effect of light-emitting diodes (LEDs) on the expression levels of phenylpropanoid biosynthetic pathway genes and the accumulation of phenylpropanoid compounds such as RA and tilianin in A. rugosa. Real-time PCR analysis showed that the phenylpropanoid pathway genes responded to the LED lights. The transcript levels of downstream genes (C4H, CHS, CHI, and RAS) were comparatively higher than those of upstream genes (PAL, TAT, and HPPR). In addition, HPLC analysis showed that the content of RA and tilianin were significantly higher in plants cultivated under white light than those grown under red, blue, green, and orange lights. The RA and tilianin content were the highest in the plantlets after three weeks of exposure to white light. These results suggested that white LED lights significantly enhanced the accumulation of phenylpropanoid compounds in A. rugosa.

Từ khóa


Tài liệu tham khảo

Ahn B, Yang CB (1991) Volatile flavor components of Bangah (Agastache rugosa O. Kuntze) Herb. Kor J Food Sci Technol 23:582–586

Hong JJ, Choi JH, Oh SR, Lee HK, Park JH, Lee KY, Kim JJ, Jeong TS, Oh GT (2001) Inhibition of cytokine-induced vascular cell adhesion molecule-1 expression: possible mechanism for anti-atherogenic effect of Agastache rugosa. FEBS Lett 495:142–147. https://doi.org/10.1016/S0014-5793(01)02379-1

Lee C, Kim H, Kho Y (2002) Agastinol and agastenol, novel lignans from Agastache rugosa and their evaluation in an apoptosis inhibition assay. J Nat Prod 65:414–416. https://doi.org/10.1021/np010425e

Kim HK, Lee HK, Shin CG, Huh H (1999) HIV integrase inhibitory activity of Agastache rugosa. Arch Pharm Res 22:520–523. https://doi.org/10.1007/bf02979163

Shin S, Kang CA (2003) Antifungal activity of the essential oil of Agastache rugosa Kuntze and its synergism with ketoconazole. Lett Appl Microbiol 36:111–115. https://doi.org/10.1046/j.1472-765X.2003.01271.x

Choi KS, Lee HY (1999) Characteristics of useful components in the leaves of baechohyang (Agastache rugosa, O. Kuntze). J Kor Soc Food Sci Nutr 28:326–332

Lee HK, Oh SR, Kim JI, Kim JW, Lee CO (1995) Agastaquinone, a new cytotoxic diterpenoid quinone from Agastache rugosa. J Nat Prod 58:1718–1721. https://doi.org/10.1021/np50125a011

Han DS (1987) Triterpenes from the root of Agastache rugosa. Kor J Pharmacogn 18:50–53

Oh HM, Kang YJ, Lee YS, Park MK, Kim SH, Kim HJ, Seo HG, Lee JH, Chang KC (2006) Protein kinase G-dependent heme oxygenase-1 induction by Agastache rugosa leaf extract protects RAW264.7 cells from hydrogen peroxide-induced injury. J Ethnopharmacol 103:229–235. https://doi.org/10.1016/j.jep.2005.08.030

Hernandez-Abreu O, Castillo-Espana P, Leon-Rivera I, Ibarra-Barajas M, Villalobos-Molina R, Gonzalez-Christen J, Vergara-Galicia J, Estrada-Soto S (2009) Antihypertensive and vasorelaxant effects of tilianin isolated from Agastache mexicana are mediated by NO/cGMP pathway and potassium channel opening. Biochem Pharmacol 78:54–61. https://doi.org/10.1016/j.bcp.2009.03.016

Nam KH, Choi JH, Seo YJ, Lee YM, Won YS, Lee MR, Lee MN, Park JG, Kim YM, Kim HC, Lee CH, Lee HK, Oh SR, Oh GT (2006) Inhibitory effects of tilianin on the expression of inducible nitric oxide synthase in low density lipoprotein receptor deficiency mice. Exp Mol Med 38:445–452. https://doi.org/10.1038/emm.2006.52

Petersen M, Simmonds MSJ (2003) Molecules of interest—rosmarinic acid. Phytochemistry 62:121–125. https://doi.org/10.1016/S0031-9422(02)00513-7

Dhabi NA, Arasu MV, Park CH, Park SU (2014) Recent studies on rosmarinic acid and its biological and pharmacological activities. Excli J 13:1192–1195. https://doi.org/10.17877/DE290R-6923

Gao LP, Wei HL, Zhao HS, Xiao SY, Zheng RL (2005) Antiapoptotic and antioxidant effects of rosmarinic acid in astrocytes. Pharmazie 60:62–65

Swarup V, Ghosh J, Ghosh S, Saxena A, Basu A (2007) Antiviral and anti-inflammatory effects of rosmarinic acid in an experimental murine model of Japanese encephalitis. Antimicrob Agents Ch 51:3367–3370. https://doi.org/10.1128/Aac.00041-07

Vogt T (2010) Phenylpropanoid biosynthesis. Mol Plant 3:2–20. https://doi.org/10.1093/mp/ssp106

Jiang CG, Schommer CK, Kim SY, Suh DY (2006) Cloning and characterization of chalcone synthase from the moss, Physcomitrella patens. Phytochemistry 67:2531–2540. https://doi.org/10.1016/j.phytochem.2006.09.030

Li F, Jin Z, Qu W, Zhao D, Ma F (2006) Cloning of a cDNA encoding the Saussurea medusa chalcone isomerase and its expression in transgenic tobacco. Plant Physiol Biochem 44:455–461. https://doi.org/10.1016/j.plaphy.2006.08.006

Martens S, Mithofer A (2005) Flavones and flavone synthases. Phytochemistry 66:2399–2407. https://doi.org/10.1016/j.phytochem.2005.07.013

Kuroki G, Poulton JE (1981) The para-O-methylation of apigenin to acacetin by cell-free extracts of Robinia pseudoacacia L. Z Naturforsch 36:916–920. https://doi.org/10.1515/znc-1981-11-1202

Ogata J, Itoh Y, Ishida M, Yoshida H, Ozeki Y (2004) Cloning and heterologous expression of cDNAs encoding flavonoid glucosyltransferases from Dianthus caryophyllus. Plant Biotechnol 21:367–375. https://doi.org/10.5511/plantbiotechnology.21.367

Petersen MS (1991) Characterization of rosmarinic acid synthase from cell cultures of Coleus blumei. Phytochemistry 30:2877–2881. https://doi.org/10.1016/S0031-9422(00)98217-7

Petersen M (1997) Cytochrome P450-dependent hydroxylation in the biosynthesis of rosmarinic acid in Coleus. Phytochemistry 45:1165–1172. https://doi.org/10.1016/S0031-9422(97)00135-0

Ncube B, Finnie JF, Van Staden J (2012) Quality from the field: the impact of environmental factors as quality determinants in medicinal plants. S Afr J Bot 82:11–20. https://doi.org/10.1016/j.sajb.2012.05.009

Cuong D, Ha TW, Park CH, Kim NS, Yeo HJ, Chun SW, Kim C, Park SU (2019) Effects of LED lights on expression of genes involved in phenylpropanoid biosynthesis and accumulation of phenylpropanoids in wheat sprout. Agronomy-Basel. https://doi.org/10.3390/agronomy9060307

Lian TT, Cha SY, Moe MM, Kim YJ, Bang KS (2019) Effects of different colored LEDs on the enhancement of biologically active ingredients in callus cultures of Gynura procumbens (Lour.) Merr. Molecules. https://doi.org/10.3390/molecules24234336

Lee SW, Seo JM, Lee MK, Chun JH, Antonisamy P, Arasu MV, Suzuki T, Al-Dhabi NA, Kim SJ (2014) Influence of different LED lamps on the production of phenolic compounds in common and Tartary buckwheat sprouts. Ind Crop Prod 54:320–326. https://doi.org/10.1016/j.indcrop.2014.01.024

Okamoto K, Yanagi T, Kondo S (1997) Growth and morphogenesis of lettuce seedlings raised under different combinations of red and blue light. Acta Hortic. https://doi.org/10.17660/actahortic.1997.435.14

Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsicum annuum L) grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot-London 79:273–282. https://doi.org/10.1006/anbo.1996.0341

Seo JM, Arasu MV, Kim YB, Park SU, Kim SJ (2015) Phenylalanine and LED lights enhance phenolic compound production in tartary buckwheat sprouts. Food Chem 177:204–213. https://doi.org/10.1016/j.foodchem.2014.12.094

Thwe AA, Kim YB, Li X, Seo JM, Kim SJ, Suzuki T, Chung SO, Park SU (2014) Effects of light-emitting diodes on expression of phenylpropanoid biosynthetic genes and accumulation of phenylpropanoids in Fagopyrum tataricum sprouts. J Agric Food Chem 62:4839–4845. https://doi.org/10.1021/jf501335q

Tuan PA, Thwe AA, Kim YB, Kim JK, Kim SJ, Lee S, Chung SO, Park SU (2013) Effects of white, blue, and red light-emitting diodes on carotenoid biosynthetic gene expression levels and carotenoid accumulation in sprouts of tartary buckwheat (Fagopyrum tataricum Gaertn.). J Agric Food Chem 61:12356–12361. https://doi.org/10.1021/jf4039937

Li X, Thwe AA, Park NI, Suzuki T, Kim SJ, Park SU (2012) Accumulation of phenylpropanoids and correlated gene expression during the development of tartary buckwheat sprouts. J Agric Food Chem 60:5629–5635. https://doi.org/10.1021/jf301449a

OuYang FQ, Mao JF, Wang JH, Zhang SG, Li Y (2015) Transcriptome analysis reveals that red and blue light regulate growth and phytohormone metabolism in Norway spruce [Picea abies (L.) Karst.]. PLoS ONE. https://doi.org/10.1371/journal.pone.0127896

Johkan M, Shoji K, Goto F, Hashida S, Yoshihara T (2010) Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience 45:1809–1814. https://doi.org/10.21273/Hortsci.45.12.1809

Kim YJ, Kim YB, Li X, Choi SR, Park S, Park JS, Lim YP, Park SU (2015) Accumulation of phenylpropanoids by white, blue, and red light irradiation and their organ-specific distribution in Chinese cabbage (Brassica rapa ssp Pekinensis). J Agric Food Chem 63:6772–6778. https://doi.org/10.1021/acs.jafc.5b02086

Ma G, Zhang LC, Kato M, Yamawaki K, Kiriiwa Y, Yahata M, Ikoma Y, Matsumoto H (2012) Effect of blue and red LED light irradiation on β-cryptoxanthin accumulation in the flavedo of citrus fruits. J Agric Food Chem 60:197–201. https://doi.org/10.1021/jf203364m

Zhang LC, Ma G, Kato M, Yamawaki K, Takagi T, Kiriiwa Y, Ikoma Y, Matsumoto H, Yoshioka T, Nesumi H (2012) Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro. J Exp Bot 63:871–886. https://doi.org/10.1093/jxb/err318

Rani A, Singh K, Sood P, Kumar S, Ahuja PS (2009) p-Coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze]. Funct Integr Genomic 9:271–275. https://doi.org/10.1007/s10142-008-0098-3

Singh K, Kumar S, Rani A, Gulati A, Ahuja P (2009) Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Funct Integr Genomic 9:125–134. https://doi.org/10.1007/s10142-008-0092-9

Xu F, Cai R, Cheng SY, Du HW, Wang Y, Cheng SH (2008) Molecular cloning, characterization and expression of phenylalanine ammonia-lyase gene from Ginkgo biloba. Afr J Biotechnol 7:721–729

Tuan PA, Park WT, Xu H, Park NI, Park SU (2012) Accumulation of tilianin and rosmarinic acid and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. J Agric Food Chem 60:5945–5951. https://doi.org/10.1021/jf300833m

Hu YS, Zhang L, Di P, Chen WS (2009) Cloning and induction of phenylalanine ammonia-lyase gene from Salvia miltiorrhiza and its effect on hydrophilic phenolic acids levels. Chin J Nat Med 7:0449–0457

Song J, Wang ZZ (2011) RNAi-mediated suppression of the phenylalanine ammonia-lyase gene in Salvia miltiorrhiza causes abnormal phenotypes and a reduction in rosmarinic acid biosynthesis. J Plant Res 124:183–192. https://doi.org/10.1007/s10265-010-0350-5

Weitzel C, Petersen M (2010) Enzymes of phenylpropanoid metabolism in the important medicinal plant Melissa officinalis L. Planta 232:731–742. https://doi.org/10.1007/s00425-010-1206-x

Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM (2013) The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata). Sci Hortic-Amsterdam 150:86–91. https://doi.org/10.1016/j.scienta.2012.10.002

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