Influence of harvest season and different polarity solvents on biological activities, phenolic compounds and lipid-soluble pigment contents of Spirogyra sp. from Algeria
Tóm tắt
Effects of extraction solvent and harvest period on phytochemical analysis and antioxidant and antimicrobial activities of extracts obtained from the freshwater macroalga, Spirogyra sp. were evaluated. The experiment lasted five months, where five crude extracts were obtained by maceration using different solvents: hexane, chloroform, dichloromethane, acetone and methanol. Higher values of yields were recorded in methanolic extracts on February (7.304%) and March (4.812%). All extracts were rich in proanthocyanidins compared to total phenolics and flavonoids. The total amounts of carotenoids were very low (0.060 ± 0001 to 0.171 ± 0.005 mg/g) compared to chlorophylls which were higher in February (6.365 ± 0.075 mg/g) and in March (5.501 ± 0.118 mg/g). The antioxidant activities indicated that the chloroformic and dichloromethanic extracts of February and March exhibited a good activity. The correlation using the Pearson test of phenolic compounds and antioxidant activity tests have confirmed the strong contribution of flavonoids and proanthocyanidins. The greatest antimicrobial activity was found in hexanic extracts at 0.1 to 1 mg/disk. The present findings suggest that Spirogyra sp. can be exploited not only for human health, but also for screening new economic natural antioxidants that could be used in foods, cosmetics and pharmaceutical industries.
Tài liệu tham khảo
Abdel-Aal EI, Haroon AM, Mofeed J (2015) Successive solvent extraction and GC–MS analysis for the evaluation of the phytochemical constituents of the filamentous green alga Spirogyra longata. Egypt J Aquat Res 41(3):233–246
Champa P, Whangchai N, Jaturonglumlert S, Nakao N, Whangchai K (2016) Determination of phytochemical compound from Spirogyra sp. using ultrasonic assisted extraction. Int J Geomate 11(2):2391–2396
CLSI (2012) Performance standards for antimicrobial disk susceptibility tests; approved standard. CLSI document M02eA11, 11th edn. Clinical and Laboratory Standards Institute, Wayne, PA
Dash P, Tripathy NK, Padhi SB (2014) Novel antioxidant production by Cladophora sp. and Spirogyra sp. Med Sci 7(25):74–78
Demirel Z, Yilmaz-Koz FF, Karabay-Yavasoglu UN, Ozdemir G, Sukatar A (2009) Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. J Serb Chem Soc 74(6):619–628
Fabrowska J, Messyasz B, Pankiewicz R, Wilinska P, Leska B (2018) Seasonal differences in the content of phenols and pigments in thalli of freshwater Cladophora glomerata and its habitat. Water Res 135:66–74
Hainz R, Wober C, Schagerl M (2009) The relationship between Spirogyra (Zygnematophyceae, Streptophyta) filament type groups and environmental conditions in Central Europe. Aquat Bot 91(3):173–180
Ivanova AJ, Nechev I, Tsvetkova H, Najdenski K, Popov SS (2011) Compounds with antibacterial activity from the freshwater alga Spirogyra crassa (L.) Kutz. Genet Plant Physiol 1:31–37
John DM, Whitton BA, Brook AJ (2002) The freshwater algal flora of the British Isles. Cambridge University Press, Cambridge, United Kingdom
Julkunen-Titto R (1985) Phenolic constituents in the leaves of northem wiliows methods for the analysis of certain phenoics. J Agric Food Chem 33(2):213–217
Junthip R, Amornlerdpison D, Chimsook T (2013) Phytochemical screening, antioxidant activity and total phenolic content of Spirogyra spp. Adv Mater Res 699:693–697
Kamble S, Rokde A, Chavan A (2012) Antifungal activity of algal extracts against plant pathogenic fungi. Int Multidiscip Res J 2(3):23–24
Kamenarska ZG, Dimitrova-Konaklieva SD, Nikolova C, Kujumgiev AI, Stefanov KL, Popov SS (2000) Volatile components of the freshwater algae Spirogyra and Mougeotia. Z Naturforsch C 55(7–8):495–499
Kang N, Lee JH, Lee WW, Ko JY, Kim EA, Kim JS, Heu MS, Kim GH, Jeon YJ (2015) Gallic acid isolated from Spirogyra sp. improves cardiovascular disease through a vasorelaxant and antihypertensive effect. Environ Toxicol Pharmacol 39(2):764–772
Khalid MN, Shameel M, Ghazala B (2012) Bioactivity and phycochemistry of two species of Spirogyra link (Zygnemophyceae) from Pakistan. Int J Algae 14(3):237–246
Kirk JTO, Allen RL (1965) Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochem Biophys Res Commun 21:523–530
Kumar J, Dhar P, Tayade AB, Gupta D, Chaurasia OP, Upreti DK, Toppo K, Arora R, Suseela MR, Srivastava RB (2015) Chemical composition and biological activities of trans-Himalayan Alga Spirogyraporticalis (Muell.) cleve. PLoS ONE 10(2):1–24
Lee J-H, Han JW, Ko JY, Lee W, Ahn G, Kim CW, Kim GH, Jeon YG (2016) Protective effect of a freshwater alga, Spirogyra sp., against lipid peroxidation in vivo zebrafish and purification of antioxidative compounds using preparative centrifugal partition chromatography. J Appl Phycol 28(1):181–189
Lima-Filho JVM, Carvalho AFFU, Freitas SM, Melo VMM (2002) Antibacterial activity of extracts of six macroalgae from the northeastern Brazilian coast. Braz J Microbiol 33(4):311–313
Mishra VK, Bacheti RK, Husen A (2011) Medicinal Uses of Chlorophyll: a critical overview. In: Hua L and Elisa S (eds) Chlorophyll: structure, function and medicinal uses, Nova Science Publishers, Inc., Hauppauge, NY 11788 (ISBN 978-1-62100-015-0), pp 177–196
Mitova M, Usov A, Bilan M, Stefanov K, Dimitrova-Konaklieva S, Tonov D, Popov S (1999) Sterols and polysacharides in freshwater algae Spirogyra and Mougeotia. Z Naturforsch C 54(12):1016–1020
Nabavi SF, Nabavi SM, Ebrahimzadeh MA, Jafari N, Yazdanpanah S (2013) Biological activities of freshwater algae, Spirogyra singularis nordstedt. J Aquat Food Prod Technol 22(1):58–65
Oyaizu M (1986) Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 44(6):307–315
Pacheco R, Ferreira AF, Pinto T, Nobre BP, Loureiro D, Moura P, Gouveia L, Silva CM (2015) The production of pigments & hydrogen through a Spirogyra sp. Biorefinery Energy Convers Manag 89:789–797
Peerapornpisal Y (2008) Edible freshwater macroalgae in Northern Thailand. J Fish Technol Res 1:178–189
Pinto T, Gouveia L, Ortigueira J, Saratale GD, Moura P (2018) Enhancement of fermentative hydrogen production from Spirogyra sp. by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model. J Biosci Bioeng 126(2):226–234
Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269(2):337–341
Ramaraj R, Unpaprom Y, Whangchai N, Dussadee N (2015) Culture of macroalgae Spirogyra ellipsospora for long-term experiments, stock maintenance and biogas production. Emerg Life Sci Res 1(1):38–45
Rutikanga A, Gitu LM, Oyaro N (2014) Mineral composition, antioxidants and antimicrobial activities of freshwater algae (Spirogyra genus) from Jomo Kenyatta University of Agriculture and Technology (JKUAT). World Rural Observ 6:86–91
Sadasivam S, Manickam A (1996) Biochemical methods, 2nd edn. A New Age International, New Delhi, India, pp 190–191
Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 76(2):270–276
Sastry VMVS, Rao GRK (1994) Antibacterial substances from marine algae: Successive extraction using benzene, chloroform and methanol. Bot Mar 37(4): 357–360
Singleton VL, Rossi JR (1965) Colorimetry of total phenolics with phosphomolybdic–phosphothungstic acid. Am J Enol Vitic 16:144–215
Stirk WA, Reinecke DL, Staden J (2007) Seasonal variation in antifungal, antibacterial and acetyl cholinesterase activity in seven South African seaweeds. J Appl Phycol 19(3):271–276
Taherpour A, Archangi B, Ghaemmaghami S, Zolgharnein H, Ghanemi K (2016) Screening of marine algae (Padina sp.) from the Lengeh Port, Persian Gulf for antibacterial and antifungal activities. J Coast Life Med 4(9):698–702
Thumvijit T, Thuschana W, Amornlerdpison D, Peerapornpisal Y, Wongpoomchai R (2013a) Evaluation of hepatic antioxidant capacities of Spirogyra neglecta (Hassall) Kützing in rats. Interdiscip Toxicol 6(3):152–156
Thumvijit T, Inboot W, Peerapornpisal Y, Amornlerdpison D, Wongpoomchai R (2013b) The antimutagenic and antioxidant properties of Spirogyra neglecta (Hassall) Kützing. J Med Plants Res 7(34):2494–2500
Tipnee S, Ramaraj R, Unpaprom Y (2015) Nutritional evaluation of edible freshwater green Macroalga Spirogyra varians. Emerg Life Sci Res 1(2):1–7
Tuney I, Cadirci BH, Unal D, Sukatar A (2006) Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey). Turk J Biol 30(3):171–175
Vijaya Parthasarathy MD, Prema M, Krishnamurthy A (2004) Preliminary report on the antibacterial activity of extracts from Spirogyra sp. Zygnematacea-Chlorophyceae Indian Hydrobiol 7:229–233
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64(4):555–559