Influence of antigen challenge on platelet responsiveness in horses with chronic obstructive pulmonary disease

Equine Veterinary Journal - Tập 29 Số 5 - Trang 382-386 - 1997
J. M. ABLETT1,2, S. M. FAIRBAIRN2,3, Clive P. Page4, P. Lees2, F.M. Cunningham2
1Combe Down, Bath, Avon BA2 5QY, UK.
2Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Herts, AL9 7TA
3Medical Department, Merck Sharp and Dohme, Hoddesdon, Herts EN11 9BU, UK.
4Department of Pharmacology, King's College London, Manresa Road, London SW3 6LX, UK

Tóm tắt

Summary

A role for platelets in allergic airways disease has been postulated and changes in the responsiveness of circulating platelets have been demonstrated following antigen challenge of asthmatic human subjects. In this study agonist‐induced aggregation of equine platelets in vitro has been compared before and after exposure of horses to a controlled hay and straw challenge. Prior to challenge the response of platelets, from horses with chronic obstructive pulmonary disease (COPD) and normal animals, to adenosine diphosphate (ADP) and platelet activating factor (PAF) did not differ. Five hours after initiation of the challenge, there was a modest but significant decrease in the response of platelets from the COPD horses to PAF, but not to ADP. Platelets from normal horses were not less sensitive to either agonist at this time. Twenty four hours after challenge the responses of platelets from COPD horses to both agonists were the same as pre‐challenge values. These results demonstrate that antigen challenge alters the responsiveness of platelets from allergic horses to PAF and this change is suggestive of PAF release accompanying allergen exposure in the horse.

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Tài liệu tham khảo

10.1016/0022-2828(92)91081-F

Angle M.J., 1987, Ontogeny of the responsiveness to intravenous platelet‐activating factor, Lab. Invest., 57, 321

10.1159/000236857

Blank M.L., 1981, A specific acetylhydrolase for l‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphocholine (a hypotensive platelet‐activating lipid), J. Biol. Chem., 256, 175, 10.1016/S0021-9258(19)70115-X

Bussolino F., 1988, Synthesis and release of platelet‐activating factor by human vascular endothelial cells treated with tumour necrosis factor or interleukin‐1 alpha, J. Biol. Chem., 263, 11856, 10.1016/S0021-9258(18)37865-7

10.1111/j.2042-3306.1991.tb02736.x

10.1084/jem.164.1.72

Chao F.C., 1982, Alteration in plasma proteins and platelet functions with aging and cigarette smoking in healthy men, Thromb. Haemostas., 47, 259, 10.1055/s-0038-1657181

10.1164/ajrccm/142.3.587

Derksen F.J., 1985, Bronchoalveolar lavage in ponies with recurrent airway obstruction (heaves), Am Rev. respir. Dis., 132, 1066

10.1111/j.1365-2222.1993.tb00259.x

10.1111/j.2042-3306.1992.tb02815.x

Gray P.R., 1989, The role of cyclooxygenase products in the acute airway obstruction and airway hyperreactivity of ponies with heaves, Equine vet. J., 21, 145

Gresele P., 1987, Platelets. Platelet Activating Factor and Asthma. Agents and Actions, 119

10.1016/0091-6749(93)90347-I

10.1016/0091-6749(89)90117-6

Henson P.M., 1977, Basophil‐derived platelet activating factor as an in vivo mediator of acute allergic reactions: demonstration of specific desensitisation of platelets to PAF during IgE anaphylaxis in the rabbit, J. Immunol., 119, 2179, 10.4049/jimmunol.119.6.2179

10.1016/B978-012390120-0/50003-4

10.1038/253355a0

10.1002/eji.1830160318

Lynch J.M., 1986, The intracellular retention of newly synthesised platelet‐activating factor, J. Immunol., 137, 2653, 10.4049/jimmunol.137.8.2653

10.1016/0165-2427(93)90020-5

10.1016/0024-3205(95)02306-2

10.1007/BF01999637

10.1016/0034-5288(93)90063-L

10.1016/S0007-1935(96)80101-1

10.1007/BF00917823

Thompson J.M., 1984, Platelets, platelet activating factor and asthma, Am. Rev. respir. Dis., 129, A3

10.1083/jcb.110.2.529

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Equine Veterinary Journal

Tập 29 Số 5

382-386

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