Xiaocui Luo1, Zhenhuang Chen1, Jianbo Xue1, Dong-Xiao Zhao1, Chen Lǚ1, Yihong Li1, Songmin Li1, Ya-Wen Du1, Qun Liu1, Ping Wang2,1, Mingyuan Liu3,4, Liquan Huang1,5
1College of Life Sciences, Zhejiang University, 310058 Hangzhou, China
2Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China
3College of Veterinary Medicine, Jilin University, 130062 Changchun, China; and
4Key Lab of Zoonosis Research, Institute of Zoonosis, Jilin University, 130062 Changchun, China;
5Monell Chemical Senses Center, Philadelphia, PA 19104.
Tóm tắt
SignificanceIntestinal tuft cells are sentinels monitoring the luminal contents and play a critical role in type 2 immunity. In this work,Trichinella spiralisexcretion–secretion and extract were shown to directly induce interleukin 25 (IL-25) release from the intestinal villi, evoke calcium responses in tuft cells, and activate Tas2r bitter-taste receptors, whereas the bitter compound salicin was shown to activate and induce tuft cells to release IL-25. Gα-gustducin/Gβ1γ13 and/or Gαo/Gβ1γ13, Plcβ2, Ip3r2, and Trpm5 comprise the signal transduction pathways that tuft cells utilize to initiate type 2 immune responses. Potentiation of Trpm5 by a natural sweet compound, stevioside, can enhance the tuft cell–ILC2 circuit’s activity, indicating that modulating these signaling components can help devise new means of combating parasites.
