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Kích thích khả năng chịu lạnh trong lúa mì trong quá trình nảy mầm bằng cách ngâm hạt trong nitric oxide và gibberellin
Tóm tắt
Sự lạnh có thể làm ảnh hưởng đến quá trình nảy mầm và sự phát triển của cây con, gây trở ngại lớn cho việc hình thành năng suất hạt ngũ cốc trong vụ mùa đông trồng muộn. Hạt lúa mì đông (Triticum aestivum L.) đã được ngâm riêng biệt với natri nitroprusside (SNP, như một chất cho nitric oxide) và axit gibberellic (GA3) trước khi nảy mầm, sau đó nảy mầm dưới nhiệt độ thấp. Việc tiền xử lý bằng SNP và GA3 làm tăng tỷ lệ nảy mầm, chỉ số nảy mầm, trọng lượng và chiều dài của cotyledon và rễ, trong khi giảm thời gian nảy mầm trung bình và trọng lượng của hạt nảy mầm trong điều kiện nhiệt độ thấp. NO ngoại sinh và GA3 làm tăng tỷ lệ hô hấp của hạt và thúc đẩy quá trình phân giải tinh bột kèm theo việc tăng hoạt động của amylase. Bên cạnh đó, các hệ thống chống oxy hóa hiệu quả đã được kích hoạt bởi NO, và qua đó giảm thiểu nồng độ malondialdehyde và hydrogen peroxide (H2O2). Sự phát triển của cây con cũng được thúc đẩy bởi NO ngoại sinh và GA3 nhờ vào quá trình nảy mầm hạt được cải thiện và duy trì môi trường cân bằng của các loài oxy phản ứng trong điều kiện cây con phát triển dưới nhiệt độ lạnh. Điều này cho thấy NO ngoại sinh có hiệu quả hơn GA3 trong việc giảm căng thẳng do lạnh trong quá trình nảy mầm và thiết lập cây con ở lúa mì.
Từ khóa
#lúa mì #chịu lạnh #nảy mầm #nitric oxide #gibberellin #hạt giống #phát triển cây conTài liệu tham khảo
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