Induction of IL-8 in periodontal ligament cells by H2O2
Tóm tắt
Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H2O2, one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H2O2. IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH2-terminal kinase (JNK) was estimated by Western blotting. Treatment with H2O2 at concentration of up to 250 μM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 μM H2O2 did not increase IL-8 production. Catalase, an inhibitor of H2O2, down-regulated the production of IL-8 induced by H2O2. H2O2 increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H2O2. These results indicate that H2O2 acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H2O2 deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells.
Tài liệu tham khảo
Baggiolini, M. and I. Clark-Lewis. 1992. Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett. 307, 97–101.
Baggiolini, M., A. Walz, and S.L. Kunkel. 1989. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J. Clin. Invest. 84, 1045–1049.
Bartold, P.M., O.W. Wiebkin, and J.C. Thonard. 1984. The effect of oxygen-derived free radicals on gingival proteoglycans and hyaluronic acid. J. Periodontal Res. 19, 390–400.
Bendre, M.S., A.G. Margulies, B. Walser, N.S. Akel, S. Bhattacharrya, R.A. Skinner, F. Swain, V. Ramani, K.S. Mohammad, L.L. Wessner, A. Martinez, T.A. Guise, J.M. Chirgwin, D. Gaddy, and L.J. Suva. 2005. Tumor-derived interleukin-8 stimulates osteolysis independent of the receptor activator of nuclear factor-κB ligand pathway. Cancer Res. 65, 11001–11009.
Bendre, M.S., D.C. Montague, T. Peery, N.S. Akel, D. Gaddy, and L.J. Suva. 2003. Interleukin-8 stimulation of osteoclastogenesis and bone resorption is a mechanism for the increased osteolysis of metastatic bone disease. Bone 33, 28–37.
DeForge, L.E., A.M. Preston, E. Takeuchi, J. Kenney, L.A. Boxer, and D.G. Remick. 1993. Regulation of interleukin 8 gene expression by oxidant stress. J. Biol. Chem. 268, 25568–25576.
Di Paola, R., E. Mazzon, D. Zito, D. Maiere, D. Britti, T. Genovese, and S. Cuzzocrea. 2005. Effects of tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis. J. Clin. Periodontol. 32, 1062–1068.
Dröge, W. 2002. Free radicals in the physiological control of cell function. Physiol. Rev. 82, 47–95.
Gamonal, J., A. Acevedo, A. Bascones, O. Jorge, and A. Silva. 2000. Levels of interleukin-1β,-8, and-10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J. Periodontol. 71, 1535–1545.
Gamonal, J., A. Acevedo, A. Bascones, O. Jorge, and A. Silva. 2001. Characterization of cellular infiltrate, detection of chemokine receptor CCR5 and interleukin-8 and RANTES chemokines in adult periodontitis. J. Periodon. Res. 36, 194–203.
Genestra, M. 2007. Oxyl radicals, redox-sensitive signalling cascades and antioxidants. Cell. Signal. 19, 1807–1819.
Hwang, Y.S., M. Jeong, J.S. Park, M.H. Kim, D.B. Lee, B.A. Shin, N. Mukaida, L.M. Ellis, H.R. Kim, B.W. Ahn, and Y.D. Jung. 2004. Interleukin-1β stimulates IL-8 expression through MAP kinase and ROS signaling in human gastric carcinoma cells. Oncogene 23, 6603–6611.
Ishikawa, I. 2007. Host responses in periodontal diseases: A preview. Periodontol 2000. 43, 9–13.
Josse, C., J.R. Boelaert, M. Best-Belpomme, and J. Piette. 2001. Importance of post-transcriptional regulation of chemokine genes by oxidative stress. Biochem. J. 360, 321–333.
Kimura, S., T. Yonemura, and H. Kaya. 1993. Increased oxidative product formation by peripheral blood polymorphonuclear leukocytes in human periodontal diseases. J. Periodontal Res. 28, 197–203.
Kumar, V., A.K. Abbas, and N. Fausto. 2005. Robbins and Cotran Pathologic Basis of Disease, 7th ed., p. 53–61. Elsevier Inc., Philadelphia, PA, USA.
Lakshminarayanan, V., D.W. Beno, R.H. Costa, and K.A. Roebuck. 1997. Differential regulation of interleukin-8 and intercellular adhesion molecule-1 by H2O2 and tumor necrosis factor-α in endothelial and epithelial cells. J. Biol. Chem. 272, 32910–32918.
Lee, H.J., J.W. Cho, S.C. Kim, K.H. Kang, S.K. Lee, S.H. Pi, S.K. Lee, and E.C. Kim. 2006. Roles of p38 and ERK MAP kin-ases in IL-8 expression in TNF-α-and dexamethasone-stimulated human periodontal ligament cells. Cytokine 35, 67–76.
Lee, S.H., K.K. Kim, and B.K. Choi. 2005. Upregulation of intercellular adhesion molecule 1 and proinflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections. Infect. Immun. 73, 268–276.
Liu, R.K., C.F. Cao, H.X. Meng, and Y. Gao. 2001. Polymorphonuclear neutrophils and their mediators in gingival tissues from generalized aggressive periodontitis. J. Periodontol. 72, 1545–1553.
Long, P., J. Hu, N. Piesco, M. Buckley, and S. Agarwal. 2001. Low magnitude of tensile strain inhibits IL-1β-dependent induction of pro-inflammatory cytokines and induces synthesis of IL-10 in human periodontal ligament cells in vitro. J. Dent. Res. 80, 1416–1420.
Pelaia, G., G. Cuda, A. Vatrella, L. Gallelli, D. Fratto, V. Gioffre, B. D’Agostino, M. Caputi, R. Maselli, F. Rossi, F.S. Costanzo, and S.A. Marsico. 2004. Effects of hydrogen peroxide on MAPK activation, IL-8 production and cell viability in primary cultures of human bronchial epithelial cells. J. Cell. Biochem. 93, 142–152.
Sculley, D.V. and S.C. Langley-Evans. 2002. Salivary antioxidants and periodontal disease status. Proc. Nutr. Soc. 61, 137–143.
Sheikhi, M., R.K. Bouhafs, K.J. Hammarstrom, and C. Jarstrand. 2001. Lipid peroxidation caused by oxygen radicals from Fusobacterium-stimulated neutrophils as a possible model for the emergence of periodontitis. Oral Dis. 7, 41–46.
Sheikhi, M., A. Gustafsson, and C. Jarstrand. 2000. Cytokine, elastase and oxygen radical release by Fusobacterium nucleatum-activated leukocytes: A possible pathogenic factor in periodontitis. J. Clin. Periodontol. 27, 758–762.
Tanaka, S. 2007. Signaling axis in osteoclast biology and therapeutic targeting in the RANKL/RANK/OPG system. Am. J. Nephrol. 27, 466–478.
Thannickal, V.J. and B.L. Fanburg. 2000. Reactive oxygen species in cell signaling. Am. J. Physiol. Lung Cell. Mol. Physiol. 279, L1005–L1028.
Tsai, C.C., H.S. Chen, S.L. Chen, Y.P. Ho, K.Y. Ho, Y.M. Wu, and C.C. Hung. 2005. Lipid peroxidation: A possible role in the induction and progression of chronic periodontitis. J. Periodontal Res. 40, 378–384.
Tsai, C.C., Y.P. Ho, and C.C. Chen. 1995. Levels of interleukin-1β and interleukin-8 in gingival crevicular fluids in adult periodontitis. J. Periodontol. 66, 852–859.
Valko, M., D. Leibfritz, J. Moncol, M.T. Cronin, M. Mazur, and J. Telser. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell. Biol. 39, 44–84.
Valko, M., C.J. Rhodes, J. Moncol, M. Izakovic, and M. Mazur. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem. Biol. Interact. 160, 1–40.
Verhasselt, V., M. Goldman, and F. Willems. 1998. Oxidative stress up-regulates IL-8 and TNF-α synthesis by human dendritic cells. Eur. J. Immunol. 28, 3886–3890.
Yamaji, Y., T. Kubota, K. Sasaguri, S. Sato, Y. Suzuki, H. Kumada, and T. Umemoto. 1995. Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides. Infect. Immun. 63, 3576–3581.
Yamamoto, T., M. Kita, F. Oseko, T. Nakamura, J. Imanishi, and N. Kanamura. 2006. Cytokine production in human periodontal ligament cells stimulated with Porphyromonas gingivalis. J. Periodontal Res. 41, 554–559.
Yamamoto, K., R. Kushima, O. Kisaki, Y. Fujiyama, and H. Okabe. 2003. Combined effect of hydrogen peroxide induced oxidative stress and IL-1α on IL-8 production in CaCo-2 cells (a human colon carcinoma cell line) and normal intestinal epithelial cells. Inflammation 27, 123–128.