Induced tolerance against stem-rot disease of low-land indica rice (Oryza sativa var. Manika) caused by Sclerotium oryzae Catt. in sub-lethal dose of cadmium

Journal of Plant Pathology - Tập 104 - Trang 149-165 - 2021
Tanushree Ghosh1, Chinmay Pradhan1, Anath Bandhu Das1,2
1Molecular Microbiology Laboratory, Department of Botany, Utkal University, Bhubaneswar, India
2Centre of Excellence for North East India Studies, New Academic Block, Utkal University, Bhubaneswar, India

Tóm tắt

Sclerotium oryzae Catt. stem rot in rice is a devastating disease affecting rice productivity. Administration of sub-lethal dose (50 µM) of cadmium (Cd) against the pathogenic activity of Sclerotium oryzae on low-land rice (Oryza sativa var. Manika), was investigated. Improvement of shoot length was noticed with an increase in photosynthetic pigments, total carbohydrate, sugar, protein content in co-stressed (Cd + S. oryzae) rice as compared to the pathogen (S. oryzae) treated plant. Reduction of the fungal load was observed in co-stressed rice root tissue as evident by the SEM study. Fungus-treated rice, showed 2.4 fold reductions in protein level in 30 days after inoculation while that reduction was only 1.2 fold in co-stressed plants. In co-stressed rice Cd was traced in the root but not in shoot, leaf, panicle, and grain as evident by atomic absorption spectroscopy and Raman spectrum. An increase of stress-mediated proline (~ 4.2 fold) and other metabolites were found adjusted at co-stressed rice that might be due to Cd induced defense response in rice against S. oryzae. Downregulation of enhanced phenyl ammonia-lyase (PAL) and malondialdehyde (MDA) activity in co-stressed rice as compared to Cd treated plant justifies heavy metal (sub-lethal dose) mediated activation of the defense system of rice. A significant increase in the activity of catalase, guaiacol peroxidase, and superoxide dismutase observed in the co-stressed plant as compared to the pathogen treated plant supports the concept of cellular defense mechanism in rice. Protein band of ~ 29 kDa was disappeared in S. oryze infected rice while co-stressed rice showed a new ~ 23.5 kDa band that might be due to activation of PR-protein mediated defense in rice. The effect of low-dose Cd might be useful in providing defense in paddy crops against pathogen infection.

Tài liệu tham khảo

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