Indocyanine green-incorporated exosomes for improved in vivo imaging of sentinel lymph node

Applied Biological Chemistry - Tập 59 - Trang 71-76 - 2016
Gyeonghui Yu1, Heesun Jung1, Hyejung Mok1
1Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea

Tóm tắt

For improved physical stability and fast accumulation in sentinel lymph nodes in vivo, indocyanine green (ICG) was incorporated into exosomes (EXOs) to form a novel ICG-based near-infrared fluorescence probe. Compared with free ICG, prepared ICG-TBAI-encapsulated EXOs (ICG/EXOs) smaller than 50 nm had greater physicochemical stability in aqueous solution when exposed to light at 37 °C. In addition, sentinel lymph nodes were visualized within minutes with ICG/EXOs at a low ICG dose (0.05 mg/kg) owing to its well-controlled size distribution. Taken together, the results show that ICG/EXOs are effective biocompatible and sensitive near-infrared probes for real-time sentinel lymph node imaging in vivo.

Tài liệu tham khảo

Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood MJ (2011) Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol 29(4):341–345 Cabral H, Matsumoto Y, Mizuno K, Chen Q, Murakami M, Kimura M, Terada Y, Kano MR, Miyazono K, Uesaka M, Nishiyama N, Kataoka K (2011) Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size. Nat Nanotechnol 6(12):815–823 Caradec J, Kharmate G, Hosseini-Beheshti E, Adomat H, Gleave M, Guns E (2014) Reproducibility and efficiency of serum-derived exosome extraction methods. Clin Biochem 47(13–14):1286–1292 Chi CW, Ye JZ, Ding HL, He D, Huang WH, Zhang GJ, Tian J (2013) Use of indocyanine green for detecting the sentinel lymph node in breast cancer patients: from preclinical evaluation to clinical validation. PLoS One 8(12):e83927 Desmettre T, Devoisselle JM, Mordon S (2000) Fluorescence properties and metabolic features of indocyanine green (ICG) as related to angiography. Surv Ophthalmol 45(1):15–27 Engel E, Schraml R, Maisch T, Kobuch K, Koenig B, Szeimies RM, Hillenkamp J, Baumler W, Vasold R (2008) Light-induced decomposition of indocyanine green. Invest Ophthalmol Vis Sci 49(5):1777–1783 Johnsen KB, Gudbergsson JM, Skov MN, Pilgaard L, Moos T, Duroux M (2014) A comprehensive overview of exosomes as drug delivery vehicles—Endogenous nanocarriers for targeted cancer therapy. Bba-Rev Cancer 1:75–87 Kim TH, Chen Y, Mount CW, Gombotz WR, Li X, Pun SH (2010) Evaluation of temperature-sensitive, indocyanine green-encapsulating micelles for noninvasive near-infrared tumor imaging. Pharm Res 27(9):1900–1913 Kim J, Chong Y, Mok H (2014) Shell-crosslinked hyaluronic acid nanogels for live monitoring of hyaluronidase activity in vivo. Macromol Biosci 14(6):881–888 Mok H, Jeong H, Kim SJ, Chung BH (2012) Indocyanine green encapsulated nanogels for hyaluronidase activatable and selective near infrared imaging of tumors and lymph nodes. Chem Commun (Camb) 48(69):8628–8630 Noh YW, Kong SH, Choi DY, Park HS, Yang HK, Lee HJ, Kim HC, Kang KW, Sung MH, Lim YT (2012) Near-infrared emitting polymer nanogels for efficient sentinel lymph node mapping. ACS Nano 6(9):7820–7831 Robinson HA, Kwon S, Hall MA, Rasmussen JC, Aldrich MB, Sevick-Muraca EM (2013) Non-invasive optical imaging of the lymphatic vasculature of a mouse. J Vis Exp 73:e4326 Saxena V, Sadoqi M, Shao J (2004) Enhanced photo-stability, thermal-stability and aqueous-stability of indocyanine green in polymeric nanoparticulate systems. J Photochem Photobiol, B 74(1):29–38 Sun D, Zhuang X, Xiang X, Liu Y, Zhang S, Liu C, Barnes S, Grizzle W, Miller D, Zhang HG (2010) A novel nanoparticle drug delivery system: the anti-inflammatory activity of curcumin is enhanced when encapsulated in exosomes. Mol Ther 18(9):1606–1614 Tan A, Rajadas J, Seifalian AM (2013) Exosomes as nano-theranostic delivery platforms for gene therapy. Adv Drug Deliver Rev 65(3):357–367 Twu O, de Miguel N, Lustig G, Stevens GC, Vashisht AA, Wohlschlegel JA, Johnson PJ (2013) Trichomonas vaginalis exosomes deliver cargo to host cells and mediate hostratioparasite interactions. PLoS Pathog 9(7):e1003482 Villarroya-Beltri C, Baixauli F, Gutierrez-Vazquez C, Sanchez-Madrid F, Mittelbrunn M (2014) Sorting it out: regulation of exosome loading. Semin Cancer Biol 28:3–13 Watkin N, La-Touche S (2014) Penile cancer: developments in sentinel lymph node biopsy for penile cancer. Nat Rev Urol 11(3):135–137