Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos

Campa, 2019, Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts, Nat Methods, 16, 887, 10.1038/s41592-019-0508-6

Chen, 2018, CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity, Science, 360, 436, 10.1126/science.aar6245

Gootenberg, 2018, Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6, Science, 360, 439, 10.1126/science.aaq0179

Gootenberg, 2017, Nucleic acid detection with CRISPR-Cas13a/C2c2, Science, 356, 438, 10.1126/science.aam9321

Kim, 2016, Generation of knockout mice by Cpf1-mediated gene targeting, Nat Biotechnol, 34, 808, 10.1038/nbt.3614

Kleinstiver, 2016, Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells, Nat Biotechnol, 34, 869, 10.1038/nbt.3620

Koo, 2018, CRISPR-LbCpf1 prevents choroidal neovascularization in a mouse model of age-related macular degeneration, Nat Commun, 9, 1855, 10.1038/s41467-018-04175-y

Sancar, 2004, Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints, Annu Rev Biochem, 73, 39, 10.1146/annurev.biochem.73.011303.073723

Li, 2018, CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA, Cell Res, 28, 1, 10.1016/j.yexcr.2018.03.002

Wang, 2019, The CRISPR-Cas13a gene-editing system induces collateral cleavage of RNA in glioma cells, Adv Sci, 6, 1901299, 10.1002/advs.201901299

Zetsche, 2015, Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system, Cell, 163, 759, 10.1016/j.cell.2015.09.038

Zhang, 2012, Structural basis of transcription initiation, Science (New York, NY), 338, 1076, 10.1126/science.1227786

Zuo, 2019, Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos, Science, 364, 289, 10.1126/science.aav9973