Increasing the specificity of the forensic luminol test for blood

Luminescence - Tập 16 Số 3 - Trang 251-253 - 2001
T. I. Quickenden1, Paul D. Cooper
1Department of Chemistry, The University of Western Australia, Nedlands, WA 6907, Australia. [email protected]

Tóm tắt

AbstractIt is shown that the presumptive luminol chemiluminescence test for the presence of traces of blood can be made more determinative by measuring the peak emission wavelength of the luminol chemiluminescence. When sprayed onto a surface containing traces of human haemoglobin, a 1 g/L solution of aqueous luminol containing 7 g/L sodium perborate gives an emission peak at 455 ± 2 nm, whereas the same mixture gives an emission peak at 430 ± 3 nm when sprayed onto a surface containing traces of sodium hypochlorite (household bleach). This spectral difference can readily be determined using spectroscopic equipment that either scans the spectrum before significant luminescence decay occurs or corrects the spectrum for the effects of any decay. It was found that bovine haemoglobin and human haemoglobin showed no significant spectral differences. Copyright © 2001 John Wiley & Sons, Ltd.

Từ khóa


Tài liệu tham khảo

10.1002/ange.19370500803

10.1520/JFS10031J

Kim BB, 1989, Optimization of peroxidase determination conditions in the reaction of catalytic oxidation of luminol and luciferin by hydrogen peroxide, Biotekhnolgiya, 5, 97

Seitz WR, 1973, Chemiluminescence and Bioluminescence, 427

Fernandez‐Gutierrez A, 1985, Molecular Luminescence Spectroscopy, 463

Larena A, 1983, Some chemiluminescence reactions in the presence of different metal ions, Optica Pura Y Aplicada, 16, 91

10.1021/ac60321a020

Bunn HF, 1977, Human Hemoglobins

Yeshion TE, 1991, Proceedings of the 6th International Symposium on Bioluminescence and Chemiluminescence 1990, 379

10.1002/bio.1170060309

Thông tin
Thông tin xuất bản

Luminescence

Tập 16 Số 3

251-253

Thông tin tác giả