Increasing efficiency of laser fluorescence diagnostics of microbial diseases
Tóm tắt
The laser-induced fluorescence spectra of five types of mycobacteria are studied in various associations. The results obtained are used to demonstrate the possibility of a diagnostic system for the differential diagnostics of microbial diseases. The method employed for the specific analysis of microbial species in the substrate that contain various associations is based on a regression of the spectral characteristics of the fluorescence intensity to the principal components. The diagnostic efficiency is increased when the fluorescence spectra are reconstructed with regard to the optical characteristics of the medium. The detergent effect on the parameters of microbial fluorescence diagnostics is studied.
Tài liệu tham khảo
J. Lakowiczh, Fluorescence Spectroscopy (Wiley, New York, 1982).
M. Alexandrov, A. Vorobjev, E. Pashkov, M. Philotov, et al., “The Laser Fluorescent Diagnostics in Medicine, Food, Industry, Ecology,” Electronics: NTB 3, 43 (2003).
J. A. Kloefer, R. E. Mielke, M. S. Wong, et al., “Quantum Dots as Strain-and Metabolism-Specific Microbiological Labels,” Appl. Environ. Microbiol. 69, 4205 (2003).
A. Lucas, M. Perk, Y. Wen, and C. Smith, “Fluorescence Spectroscopic Detection of Early Injury-Induced Atherosclerosis,” Proc. SPIE 1642, 183 (1992).
V. V. Sokolov, E. V. Filonenko, L. V. Telegina, et al., Quantum Electron. 32, 963 (2002).
T. Naes and H. Martens, Multivariate Calibration.