Nobuyuki Miyasaka1, Yukio Hirata1, Kenji Andò1, Kazuto Sato1, H. Morita1, Masayoshi Shichiri1, Kazuo Kanno1, Kimio Tomita1, Fumiaki Marumo1
1Department of Immunological Diseases, Medical Research Institute, and the Second Department of Surgery, Tokyo Medical and Dental University, and the Institute of Rheumatology, Tokyo Women's Medical College, Tokyo, Japan.
Tóm tắt
AbstractObjective. Endothelin‐1 (ET‐1) is a potent vasoconstrictive peptide, but its precise mechanism of action in vivo has remained unknown.Methods. We measured ET‐1 activity by radioimmunoassay, in both plasma and synovial fluid from patients with inflammatory arthritides.Results. ET‐1–like immunoreactivity was found in synovial fluid, at levels severalfold higher than those in plasma. Reverse‐phase high‐performance liquid chromatography showed an elution profile corresponding to actual ET‐1. A single class of high‐affinity binding sites for ET‐1 in cultured synovial cells was also detected. Furthermore, ET‐1 induced mild DNA synthesis in synovial cells.Conclusion. Taken together, these results indicate that ET‐1 might contribute to the synovial proliferation seen in inflammatory arthritides, in an autocrine/paracrine manner.
