Increase in the ability of human cancer cells to induce cytotoxic T lymphocytes by ultraviolet irradiation

Springer Science and Business Media LLC - Tập 37 - Trang 392-399 - 1993
Yasuiki Umezu1, Lazel B Augustus1, Dai Seito1, Kazuhiro Hayakawa1, Merric I Ross2, Omar Eton3, David A Swanson4, Kyogo Itoh1,5
1Department of Immunology, University of Texas, M.D. Anderson Cancer Center, Houston, USA
2Department of General Surgery, University of Texas, M.D. Anderson Cancer Center, Houston, USA
3Department of Medical Oncology, University of Texas, M.D. Anderson Cancer Center, Houston, USA
4Department of Urology, University of Texas M. D. Anderson Cancer Center, Houston, USA
5Department of Immunology, Kurume University School of Medicine, Kurume, Japan

Tóm tắt

The roles of ultraviolet-B (UV) radiation in the immunogenicity of human cancer cells have not been fully studied. We have investigated the effects of UV radiation on metastatic melanoma and renal cell carcinoma cells with regard to MHC antigen expression and the ability to induce cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocytes (TIL) against untreated autologous tumor cells. UV radiation respectively decreased or increased MHC class I expression of freshly isolated tumor cells or cultured tumor cells, and also decreased MHC class I expression of starved cultured tumor cells. It increased the ability of both freshly isolated and cultured tumor cells to induce CTL activity from PBMC against untreated autologous tumor cells. UV-irradiated subclones that were more susceptible to CTL lysis were more potent for CTL induction from TIL than either an untreated parental clone or a UV-irradiated subclone that was resistant to CTL lysis. In summary, UV radiation increased the ability of tumor cells to induce CTL activity without a corresponding effect on MHC antigen expression.

Tài liệu tham khảo

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