Incorporation of magnetic nanoparticle to graphene oxide via simple emulsion method and their cytotoxicity

Applied Nanoscience - Tập 9 - Trang 43-48 - 2018
Emmellie Laura Albert1,2, Muhammad Bilal Sajiman1,2, Che Azurahanim Che Abdullah1,2,3
1Department of Physics, Faculty of Science, University Putra Malaysia, Serdang, Malaysia
2Materials Synthesis and Characterization Laboratory, Institute of Advance Technology, University Putra Malaysia, Serdang, Malaysia
3Biophysics Lab, Integrated Chemical Biophysics, Faculty of Science, University Putra Malaysia, Serdang, Malaysia

Tóm tắt

Magnetite nanoparticle and graphene oxide is a promising nanoparticle that can be used in multitude of field due to their exceptional characteristic. Graphene oxide has a unique 2-D structure, and excellent chemical and physical characteristics while magnetite nanoparticle has its superparamagnetic properties which enable it to be controlled by external magnetic field. Owing to that, any new formulations of magnetic nanoparticle functionalities with graphene oxide have to be taken into consideration. In this research, magnetite nanoparticles were functionalized with graphene oxide using simple emulsion and evaporation method. All the samples were characterized by X-ray diffraction, and Fourier-transform infrared, and Raman spectroscopy. The toxicity of the nanomaterials was tested with cell viability assay (XTT) using A549 cells. The cell viability remains high within 24 h and 72 h of incubation, and when the concentration increases up to 100 µg/mL only a slight decrease of viability was observed.

Tài liệu tham khảo

Agarwal S, Zhou X, Ye F, He Q, Chen GC, Soo J, Boey F, Zhang H, Chen P (2010) Interfacing live cells with nanocarbon substrates. Langmuir 26(4):2244–2247 Ahangaran F, Hassanzadeh A, Nouri S (2013) Surface modification of Fe3O4@SiO2 microsphere by silane coupling agent. Int Nano Lett 3(1):23. https://doi.org/10.1186/2228-5326-3-23 Aliabadi M, Shagholani H, Yunessnia lehi A (2017) Synthesis of a novel biocompatible nanocomposite of graphene oxide and magnetic nanoparticles for drug delivery. Int J Biol Macromol 98:287–291. https://doi.org/10.1016/j.ijbiomac.2017.02.012 Ang PK, Chen W, Wee ATS, Loh KP (2008) Solution-gated epitaxial graphene as pH sensor. J Am Chem Soc 130(44):14392–14393 Balandin AA, Ghosh S, Bao W, Calizo I, Teweldebrhan D, Miao F, Lau CN (2008) Superior thermal conductivity of single-layer graphene. Nano Lett 8(3):902–907 Becerril HA, Mao J, Liu Z, Stoltenberg RM, Bao Z, Chen Y (2008) Evaluation of solution-processed reduced graphene oxide films as transparent conductors. ACS Nano 2(3):463–470 Booth TJ, Blake P, Nair RR, Jiang D, Hill EW, Bangert U, Bleloch A, Gass M, Novoselov KS, Katsnelson MI (2008) Macroscopic graphene membranes and their extraordinary stiffness. Nano Lett 8(8):2442–2446 Bordbar AK, Rastegari AA, Amiri R, Ranjbakhsh E, Abbasi M, Khosropour AR (2014) Characterization of modified magnetite nanoparticles for albumin immobilization. Biotechnol Res Int. https://doi.org/10.1155/2014/705068 Chang Y, Yang ST, Liu JH, Dong E, Wang Y, Cao A, Liu Y, Wang H (2011) In vitro toxicity evaluation of graphene oxide on A549 cells. Toxicol Lett 200(3):201–210. https://doi.org/10.1016/j.toxlet.2010.11.016 Das A, Pisana S, Chakraborty B, Piscanec S, Saha S, Waghmare U, Novoselov K, Krishnamurthy H, Geim A, Ferrari A (2008) Monitoring dopants by Raman scattering in an electrochemically top-gated graphene transistor. Nat Nanotechnol 3(4):nnano.2008.2067 Fakruddin M, Hossain Z, Afroz H (2012) Prospects and applications of nanobiotechnology: a medical perspective. J Nanobiotechnol 10(1):31 He F, Fan J, Ma D, Zhang L, Leung C, Chan HL (2010) The attachment of Fe3O4 nanoparticles to graphene oxide by covalent bonding. Carbon. https://doi.org/10.1016/j.carbon.2010.04.052 Hu W, Peng C, Luo W, Lv M, Li X, Li D, Huang Q, Fan C (2010) Graphene-based antibacterial paper. ACS Nano 4(7):4317–4323 Kassaee MZ, Motamedi E, Majdi M (2011a) Magnetic Fe3O4-graphene oxide/polystyrene: fabrication and characterization of a promising nanocomposite. Chem Eng J 172(1):540–549. https://doi.org/10.1016/j.cej.2011.05.093 Kassaee MZ, Motamedi E, Majdi M (2011b) Magnetic Fe3O4-graphene oxide/polystyrene: fabrication and characterization of a promising nanocomposite. Chem Eng J. https://doi.org/10.1016/j.cej.2011.05.093 Kumar ASK, Rajesh N (2013) Exploring the interesting interaction between graphene oxide, Aliquat-336 (a room temperature ionic liquid) and chromium(vi) for wastewater treatment. RSC Adv 3(8):2697–2709. https://doi.org/10.1039/C2RA22627H Kumar N, Das S, Bernhard C, Varma GD (2013) Effect of graphene oxide doping on superconducting properties of bulk MgB2. Supercond Sci Technol. https://doi.org/10.1088/0953-2048/26/9/095008 Laurent S, Forge D, Port M, Roch A, Robic C, Vander Elst L, Muller RN (2008) Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem Rev 108(6):2064–2110 Lee C, Wei X, Kysar JW, Hone J (2008) Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science 321(5887):385–388 Lu AH, Salabas EeL, Schüth F (2007) Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Ed 46(8):1222–1244 Maeda Y, Yoshino T, Matsunaga T (2009) Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells. J Mater Chem 19(35):6361–6366 Marcano DC, Kosynkin DV, Berlin JM, Sinitskii A, Sun Z, Slesarev A, Alemany LB, Lu W, Tour JM (2010) Improved synthesis of graphene oxide. ACS Nano 4(8):4806–4814. https://doi.org/10.1021/nn1006368 Muszynski R, Seger B, Kamat PV (2008) Decorating graphene sheets with gold nanoparticles. J Phys Chem C 112(14):5263–5266 Novoselov KS, Geim AK, Morozov SV, Jiang D, Zhang Y, Dubonos SV, Grigorieva IV, Firsov AA (2004) Electric field effect in atomically thin carbon films. Science 306(5696):666–669 Paulchamy B, Arthi G, Lignesh B (2015) A simple approach to stepwise synthesis of graphene oxide nanomaterial. J Nanomed Nanotechnol 6(1):1 Peng T, Sun H, Peng T, Liu B, Zhao X (2017) Structural regulation and electroconductivity change of nitrogen-doping reduced graphene oxide prepared using p-phenylene diamine as modifier. Nanomaterials 7(10):292 Schedin F, Geim A, Morozov S, Hill E, Blake P, Katsnelson M, Novoselov K (2007) Detection of individual gas molecules adsorbed on graphene. Nat Mater 6(9):652 Shen J, Hu Y, Shi M, Li N, Ma H, Ye M (2010) One step synthesis of graphene oxide—magnetic nanoparticle composite. J Phys Chem C 114(3):1498–1503 Stoller MD, Park S, Zhu Y, An J, Ruoff RS (2008) Graphene-based ultracapacitors. Nano Lett 8(10):3498–3502 Wang K, Ruan J, Song H, Zhang J, Wo Y, Guo S, Cui D (2010) Biocompatibility of graphene oxide. Nanoscale Res Lett 6(1):8. https://doi.org/10.1007/s11671-010-9751-6 Watanabe M, Yoneda M, Morohashi A, Hori Y, Okamoto D, Sato A, Kurioka D, Nittami T, Hirokawa Y, Shiraishi T, Kawai K, Kasai H, Totsuka Y (2013) Effects of Fe(3)O(4) magnetic nanoparticles on A549 Cells. Int J Mol Sci 14(8):15546–15560. https://doi.org/10.3390/ijms140815546 Wu X, Liu P (2010) Facile preparation and characterization of graphene nanosheets/polystyrene composites. Macromol Res 18(10):1008–1012 Yang X, Zhang X, Ma Y, Huang Y, Wang Y, Chen Y (2009) Superparamagnetic graphene oxide–Fe3O4 nanoparticles hybrid for controlled targeted drug carriers. J Mater Chem 19(18):2710–2714 Yoo E, Kim J, Hosono E, Zhou H-s, Kudo T, Honma I (2008) Large reversible Li storage of graphene nanosheet families for use in rechargeable lithium ion batteries. Nano Lett 8(8):2277–2282 Zhang W, Cui J, Tao CA, Wu Y, Li Z, Ma L, Wen Y, Li G (2009) A strategy for producing pure single-layer graphene sheets based on a confined self-assembly approach. Angew Chem Int Ed 48(32):5864–5868. https://doi.org/10.1002/anie.200902365 Zhang M, Jia M, Jin Y (2012) Fe3O4/reduced graphene oxide nanocomposite as high performance anode for lithium ion batteries. Appl Surf Sci 261(Supplement C):298–305. https://doi.org/10.1016/j.apsusc.2012.08.004