Incorporation of a tag helps to overcome expression variability in a recombinant host

Biotechnology Reports - Tập 11 - Trang 62-69 - 2016
Saikat Bhattacharya1, Divya Reddy1, Raja Reddy2, Asmita Sharda1, Kakoli Bose2, Sanjay Gupta1
1Epigenetics and Chromatin Biology Group, Gupta Lab, India
2Integrated Biophysics and Structural Biology Lab, Cancer Research Institute, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai 410210, MH, India

Tài liệu tham khảo

Alexeyev, 1999, Gene synthesis, bacterial expression and purification of the Rickettsia prowazekii ATP/ADP translocase, Biochim. Biophys. Acta Biomembr., 1419, 299, 10.1016/S0005-2736(99)00078-4 Behura, 2013, Codon usage bias: causative factors, quantification methods and genome-wide patterns: with emphasis on insect genomes, Biol. Rev., 88, 49, 10.1111/j.1469-185X.2012.00242.x Calderone, 1996, High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coli, J. Mol. Biol., 262, 407, 10.1006/jmbi.1996.0524 Cannarozzi, 2010, A role for codon order in translation dynamics, Cell, 141, 355, 10.1016/j.cell.2010.02.036 Chen, 1990, Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes, Nucleic Acids Res., 18, 1465, 10.1093/nar/18.6.1465 de Smit, 1990, Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis, Proc. Natl. Acad. Sci. U. S. A., 87, 7668, 10.1073/pnas.87.19.7668 Gerchman, 1994, Expression of chicken linker histones in E. coli: sources of problems and methods for overcoming some of the difficulties, Protein Expr. Purif., 5, 242, 10.1006/prep.1994.1037 Gottesman, 1996, Proteases and their targets in Escherichia coli, Annu. Rev. Genet., 30, 465, 10.1146/annurev.genet.30.1.465 Gruber, 2008, The Vienna RNA websuite, Nucleic Acids Res., 36, W70, 10.1093/nar/gkn188 Gu, 2010, A universal trend of reduced mRNA stability near the translation-initiation site in prokaryotes and eukaryotes, PLoS Comput. Biol., 6, e1000664, 10.1371/journal.pcbi.1000664 Kane, 1995, Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli, Curr. Opin. Biotechnol., 6, 494, 10.1016/0958-1669(95)80082-4 Kane, 1988, Formation of recombinant protein inclusion bodies in Escherichia coli, Trends Biotechnol., 6, 95, 10.1016/0167-7799(88)90065-0 Keller, 2012, Reduced mRNA secondary-structure stability near the start codon indicates functional genes in prokaryotes, Genome Biol. Evol., 4, 80, 10.1093/gbe/evr129 Kubo, 1989, mRNA secondary structure in an open reading frame reduces translation efficiency in Bacillus subtilis, J. Bacteriol., 171, 4080, 10.1128/jb.171.7.4080-4082.1989 Luger, 1999, Expression and purification of recombinant histones and nucleosome reconstitution, 1 Marin, 2008, Folding at the rhythm of the rare codon beat, Biotechnol. J., 3, 1047, 10.1002/biot.200800089 Morse, 1969, Dynamics of synthesis translation, and degradation of trp operon messenger RNA in E. coli, Cold Spring Harb. Symp. Quant. Biol., 34, 725, 10.1101/SQB.1969.034.01.082 Murby, 1996, Upstream strategies to minimize proteolytic degradation upon recombinant production in Escherichia coli, Protein Expr. Purif., 7, 129, 10.1006/prep.1996.0018 Rosano, 2009, Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strain, Microb. Cell Fact., 8, 41, 10.1186/1475-2859-8-41 Rosenberg a, 1993, Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system, J. Bacteriol., 175, 716, 10.1128/jb.175.3.716-722.1993 Rudolph, R., 1996. Successful protein folding on an industrial scale. Princ. Pract. protein Fold. Saunders, 2010, Synonymous codon usage influences the local protein structure observed, Nucleic Acids Res., 38, 6719, 10.1093/nar/gkq495 Sharp, 1987, The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications, Nucleic Acids Res., 15, 1281, 10.1093/nar/15.3.1281 Sinclair, 2002, Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris, Protein Expr. Purif., 26, 96, 10.1016/S1046-5928(02)00526-0 Suthakaran, 2014, Studies on lipidification of streptokinase: a novel strategy to enhance the stability and activity, Am. J. Ther., 21, 343, 10.1097/MJT.0000000000000029 Tanaka, 2004, Expression and purification of recombinant human histones, Methods, 33, 3, 10.1016/j.ymeth.2003.10.024 Tuller, 2010, An evolutionarily conserved mechanism for controlling the efficiency of protein translation, Cell, 141, 344, 10.1016/j.cell.2010.03.031 Vergani, 2002, Expression, purification, and structural characterization of human histone H4, Protein Expr. Purif., 24, 420, 10.1006/prep.2001.1601 Welch, 2009, You’re one in a googol: optimizing genes for protein expression, J. R. Soc. Interface, 6, S467, 10.1098/rsif.2008.0520.focus Zhang, 2006, mRNA secondary structure at start AUG codon is a key limiting factor for human protein expression in Escherichia coli, Biochem. Biophys. Res. Commun., 349, 69, 10.1016/j.bbrc.2006.07.209