Sự bất hoạt của Cdk1/Cyclin B trong các tế bào FT210 của chuột bị giữ lại ở kỳ giữa dẫn đến thoát khỏi quá trình phân bào mà không có phân tách nhiễm sắc thể hoặc phân chia tế bào, và cho phép tiến vào chu kỳ tế bào tiếp theo

Chromosoma - Tập 116 - Trang 215-225 - 2007
James R. Paulson1
1Department of Chemistry, University of Wisconsin-Oshkosh, Oshkosh, USA

Tóm tắt

Được biết rằng sự bất hoạt của Cdk1/Cyclin B là cần thiết để tế bào thoát khỏi quá trình phân bào. Công trình nghiên cứu này kiểm tra giả thuyết rằng sự bất hoạt của Cdk1/Cyclin B không chỉ cần thiết mà còn đủ để gây ra sự thoát khỏi phân bào và tái thiết lập trạng thái giữa chu kỳ. Giả thuyết này dự đoán rằng việc bất hoạt Cdk1 trong các tế bào bị giữ lại ở kỳ giữa sẽ kích thích quá trình chuyển tiếp từ giai đoạn M sang G1. Kết quả cho thấy rằng khi các tế bào FT210 của chuột (trong đó Cdk1 nhạy cảm với nhiệt độ) bị ngăn chặn ở kỳ giữa và sau đó được chuyển sang nhiệt độ không cho phép, chúng nhanh chóng thoát khỏi quá trình phân bào như được chứng minh bởi sự tái tạo của nhân tế bào trong giai đoạn giữa chu kỳ, sự giải nén của các nhiễm sắc thể, và sự khử phosphorylation của các histone H1 và H3. Các tế bào giữa chu kỳ kết quả là hoàn toàn bình thường xét về khả năng tiến triển thông qua một chu kỳ tế bào khác. Tuy nhiên, chúng có số lượng nhiễm sắc thể gấp đôi bình thường vì trước đó chúng đã bỏ qua quá trình chia đôi ở kỳ giữa, phân tách nhiễm sắc thể và phân chia tế bào. Những kết quả này, cùng với các quan sát khác trong tài liệu, mạnh mẽ gợi ý rằng ở các tế bào động vật có vú, sự bất hoạt của Cdk1/cyclin B là dấu hiệu kích hoạt cho việc thoát khỏi phân bào và tái thiết lập trạng thái giữa chu kỳ.

Từ khóa

#bất hoạt Cdk1/Cyclin B #thoát khỏi phân bào #chuỗi tế bào #tế bào FT210 #động vật có vú

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