In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy

Lasers in Medical Science - Tập 33 - Trang 609-617 - 2018
Pam Wen Lung1, Vamshi Krishna Tippavajhala2, Thiago de Oliveira Mendes3, Claudio A. Téllez-Soto3, Desirée Cigaran Schuck4, Carla Abdo Brohem4, Marcio Lorencini4, Airton Abrahão Martin3,5
1Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UNIVAP), São José dos Campos, Brazil
2Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, India
3Biomedical Engineering Innovation Center, Biomedical Vibrational Spectroscopy Group, Universidade Brasil-UNBr, Itaquera, Brazil
4Grupo O Boticário, São José dos Pinhais, Brazil
5Departmento de Fisica, Universidade Federal do Piaui (UFPI), Teresina, Brazil

Tóm tắt

This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.

Tài liệu tham khảo

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