In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro

Nature Protocols - Tập 2 Số 2 - Trang 329-333 - 2007
Chun-Chi Liang1, Ann Y. Park1, Jun‐Lin Guan1
1Division of Molecular Medicine and Genetics and Cell and Developmental Biology, Departments of Internal Medicine, University of Michigan Medical School, Ann Arbor, 48109, Michigan, USA

Tóm tắt

Từ khóa


Tài liệu tham khảo

Todaro, G.j. et al. The initiation of cell division in a contact-inhibited mammalian cell line. J. Cell Physiol. 66, 325–333 (1965).

Haudenschild, C.C. et al. Endothelial regeneration. II. Restitution of endothelial continuity. Lab. Invest. 41, 407–418 (1979).

Etienne-Manneville, S. et al. Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta. Cell 106, 489–498 (2001).

Fukata, Y. et al. Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility. J. Cell Biol. 145, 347–361 (1999).

Abbi, S. et al. Regulation of focal adhesion kinase by a novel protein inhibitor FIP200. Mol. Biol. Cell. 13, 3178–3191 (2002).