Nhân giống in vitro bốn loài Maesa sản xuất saponin

Plant Cell, Tissue and Organ Culture - Tập 106 - Trang 215-223 - 2011
Ahmad Faizal1, Ellen Lambert1, Kenn Foubert2, Sandra Apers2, Danny Geelen1
1Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
2Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium

Tóm tắt

Đã phát triển một hệ thống nhân giống in vitro thành công cho b four loài Maesa có giá trị dược liệu khác nhau. Nhiều chồi được kích thích hình thành thông qua sự phát triển của chồi nách và tái sinh chồi từ mẫu lá. Mẫu được nuôi cấy trên môi trường Murashige và Skoog (MS) bổ sung 6-benzyladenine (BA), thidiazuron (TDZ) và/hoặc α-naphthalene acetic acid (NAA). Sự thành công trong tái sinh khác nhau tùy theo từng loài và phụ thuộc vào loại và nồng độ của các chất điều hòa sinh trưởng thực vật. Các chồi tái sinh tự phát phát triển rễ trong vòng 6 tuần trên môi trường MS không chứa hormone. Các chồi đã có rễ được chuyển đến nhà kính với tỷ lệ thành công đạt 100%. Hơn nữa, phân tích bằng phương pháp phân tích dòng tế bào chỉ ra rằng không có sự thay đổi nào về mức độ ploidy của các chồi tái sinh so với các cây trưởng thành kiểu dại. Phân tích sắc ký lớp mỏng (TLC) tiết lộ rằng các vết saponin chung và phân biệt cũng được quan sát tương tự ở các chồi tái sinh so với các cây đối chứng. Do đó, quy trình này cũng cung cấp một phương tiện hiệu quả cho việc bảo tồn in vitro các loài Maesa có khả năng sản xuất saponin có giá trị dược phẩm.

Từ khóa

#Maesa; nhân giống in vitro; saponin; chất điều hòa sinh trưởng; bảo tồn in vitro

Tài liệu tham khảo

Ataei-Azimi A, Hashemloian BD, Ebrahimzadeh H, Majd A (2008) High in vitro production of anti canceric indole alkaloids from periwinkle (Catharanthus roseus) tissue culture. Afr J Biotechnol 7:2834–2839 Bairu M, Stirk W, Dolezal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tissue Organ Cult 90:15–23 Bopana N, Saxena S (2008) In vitro propagation of a high value medicinal plant: Asparagus racemosus Willd. In Vitro Cell Dev Biol Plant 44:525–532 Carimi F, Terzi M, De Michele R, Zottini M, Lo Schiavo F (2004) High levels of the cytokinin BAP induce PCD by accelerating senescence. Plant Sci 166:963–969 Corredoira E, Ballester A, Vieitez AM (2008) Thidiazuron-induced high-frequency plant regeneration from leaf explants of Paulownia tomentosa mature trees. Plant Cell Tissue Organ Cult 95:197–208 Cousin A, Heel K, Cowling WA, Nelson MN (2009) An efficient high-throughput flow cytometric method for estimating DNA ploidy level in plants. Cytometry Part A 75A:1015–1019 De Gyves EM, Sparks CA, Fieldsend AF, Lazzeri PA, Jones HD (2001) High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Ann App Biol 138:329–332 Debnath M, Malik CP, Bisen PS (2006) Micropropagation: a tool for the production of high quality plant-based medicines. Curr Pharm Biotechno 7:33–49 Espinosa AC, Pijut PM, Michler CH (2006) Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures. HortScience 41:193–201 Feng J-C, Yu X, Shang X, Li J, Wu Y (2010) Factors influencing efficiency of shoot regeneration in Ziziphus jujuba Mill. ‘Huizao’. Plant Cell Tissue Organ Cult 101:111–117 Foubert K, Vermeersch M, Apers S, Pieters L, Maes L (2008) LC-MS analysis of 13, 28-epoxy-oleanane saponins in Maesa spp. extracts with antileishmanial activity. Planta Med 74:1086 Foubert K, Vermeersch M, Theunis M, Apers S, Cos P, Claeys M, Van Puyvelde L, Pieters L, Maes L (2009) LC-MS analysis of 13, 28-epoxy-oleanane saponins in Maesa spp. extracts with antileishmanial activity. Phytochem Anal 20:159–167 Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell-cycle in intact plant-tissues. Science 220:1049–1051 Germonprez N, Puyvelde LV, Maes L, Tri MV, Kimpe ND (2004) New pentacyclic triterpene saponins with strong anti-leishmanial activity from the leaves of Maesa balansae. Tetrahedron 60:219–228 Ghimire BK, Seong ES, Goh EJ, Kim NY, Kang WH, Kim EH, Yu CY, Chung IM (2010) High-frequency direct shoot regeneration from Drymaria cordata Willd. leaves. Plant Cell Tissue Organ Cult 100:209–217 Gonçalves S, Fernandes L, Romano A (2010) High-frequency in vitro propagation of the endangered species Tuberaria major. Plant Cell Tissue Organ Cult 101:359–363 Huetteman CA, Preece JE (1993) Thidiazuron—a potent cytokinin for woody plant-tissue culture. Plant Cell Tissue Organ Cult 33:105–119 Jones MPA, Yi ZJ, Murch SJ, Saxena PK (2007) Thidiazuron-induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems. Plant Cell Rep 26:13–19 Kaeppler SM, Kaeppler HF, Rhee Y (2000) Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol 43:179–188 Kanchanapoom K, Boonvanno K (2000) A protocol towards micropropagation of the piscicidal plant, Maesa ramentacea A. DC. ScienceAsia 26:201–205 Karuppusamy S, Kiranmai C, Aruna V, Pullaiah T (2009) In vitro conservation of Ceropegia intermedia—an endemic plant of south India. Afr J Biotechnol 8:4052–4057 Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell-cultures for plant improvement. Theor Appl Genet 60:197–214 Loureiro J, Rodriguez E, Dolezel J, Santos C (2006) Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Ann Bot 98:679–689 Lu C-Y (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol Plant 29:92–96 Maes L, Berghe DV, Germonprez N, Quirijnen L, Cos P, De Kimpe N, Van Puyvelde L (2004a) In vitro and in vivo activities of a triterpenoid saponin extract (PX-6518) from the plant Maesa balansae against visceral Leishmania species. Antimicrob Agents Ch 48:130–136 Maes L, Germonprez N, Quirijnen L, Van Puyvelde L, Cos P, Berghe DV (2004b) Comparative activities of the triterpene saponin maesabalide III and liposomal amphotericin B (AmBisome) against Leishmania donovani in hamsters. Antimicrob Agents Ch 48:2056–2060 Mallón R, Rodríguez-Oubiña J, González M (2010) In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis. Plant Cell Tissue Organ Cult 101:31–39 Mukherjee P, Husain N, Misra SC, Rao VS (2010) In vitro propagation of a grape rootstock, deGrasset (Vitis champinii Planch.): effects of medium compositions and plant growth regulators. Sci Hort 126:13–19 Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497 Murovec J, Eler K, Bohanec B (2010) Adventitious shoot regeneration from leaf and internodal explants of Mimulus Aurantiacus Curtis. Propag Ornam Plants 10:18–23 Nicasio-Torres MD, Erazo-Gomez JC, Cruz-Sosa F (2009) In vitro propagation of two antidiabetic species known as guarumbo: Cecropia obtusifolia and Cecropia peltata. Acta Physiol Plant 31:905–914 Obae S, West T (2010) Nuclear DNA content of Hydrastis canadensis L. and genome size stability of in vitro regenerated plantlets. Plant Cell Tissue Organ Cult 102:259–263 Prakash S, Van Staden J (2008) Micropropagation of Searsia dentata. In Vitro Cell Dev Biol Plant 44:338–341 Radić S, Prolić M, Pavlica M, Pevalek-Kozlina B (2005) Cytogenetic stability of Centaurea ragusina long-term culture. Plant Cell Tissue Organ Cult 82:343–348 Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotech Adv 18:91–120 Sindambiwe JB, Balde AM, De Bruyne T, Pieters L, Van den Heuvel H, Claeys M, Berghe VD, Vlietinck AJ (1996) Triterpenoid saponins from Maesa lanceolata. Phytochemistry 41:269–277 Singh S, Rathod Z, Saxena OP (2009) In vitro plant regeneration and production of saponins of Bacopa monnieri. Natl Acad Sci Lett 32:77–82 Vongpaseuth K, Roberts SC (2007) Advancements in the understanding of paclitaxel metabolism in tissue culture. Curr Pharm Biotechno 8:219–236 Vujovic T, Ruzic D, Cerovic R, Momirovic GS (2010) Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants. Plant Growth Regul 61:265–275 Wiart C (2006) Medicinal plants of Asia and the Pacific. In: Wiart C (ed) Medicinal plants of Asia and the Pacific. CRC press, Boca Raton, FL, pp 9–14 Wu J, Zhong J-J (1999) Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. J Biotechnol 68:89–99 Zheng W, Xu XD, Dai H, Chen LQ (2009) Direct regeneration of plants derived from in vitro cultured shoot tips and leaves of three Lysimachia species. Sci Hort 122:138–141 Zhou HC, Li M, Zhao X, Fan XC, Guo AG (2010) Plant regeneration from in vitro leaves of the peach rootstock ‘Nemaguard’ (Prunus persica x P. davidiana). Plant Cell Tissue Organ Cult 101:79–87