In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India
Tóm tắt
An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25–10.0 mgl−1) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5–1.0 mgl−1) auxins (NAA/IBA/IAA). 1.0 mgl−1 BAP with 0.5 mgl−1 NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl−1 NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.
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