In-vitro and in-vivo assessment of Polycaprolactone-Chitosan-Pectin imbibed nanofiber potentials as a wound healing biomaterial

Springer Science and Business Media LLC - 2023
Chitrakala Kaliaperumal1, Anitha Thulasisingh2
1Biotechnology Department, St. Peter’s College of Engineering and Technology, Avadi, Chennai, India
2Biotechnology Department, Rajalaskhmi Engineering College, Chennai, India

Tóm tắt

The goal of the current study was to evaluate the wound healing potential of electrospun Polycaprolactone—Chitosan—Pectin (PCL-CS-PEC composite nanofiber under in vitro and in vivo conditions in order to determine their appropriateness for its use as wound dressings. 15%, 17%, and 19% PCL-CS-PEC scaffolds were fabricated individually and subjected to various investigations such as hemolysis, swelling, porosity, cell viability, and in-vivo wound healing activity. The collected parameters were subjected to statistical analysis using SPSS version 25 for statistical significance. The current study highlighted that the studied different concentrations of PCL-CS-PEC were hemocompatible since their hemolysis value was found to be less than 5% which is acceptable for any scaffolds or biomaterials universally. The 19% (w/v) PCL-CS-PEC-based scaffold demonstrated the maximum porosity of above 80% across the analyzed concentrations, making it the best choice for tissue engineering applications. The swelling index revealed that 19% of PCL-CS-PEC scaffolds are identified to be superior as it showed 301.7 ± 1.5%, 652.0 ± 2.0%, 902.3 ± 2.1%, and 1151 ± 1.5% for the different time interval of 6,12,18 and 24 h correspondingly than 15% and 17% PCL-CS-PEC nanofiber. Acute dermal toxicity analysis was found to be normal. Wound contraction rate was also found to be superior with 19% PCL-CS-PEC scaffolds than other analyzed concentrations. Our study employed the synthesis of nanofiber with PCL, with an add-on of CS and PEC because PCL has already proven to be wound healing material.

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