In situ crosslinking of chlorophyll to protein. Use of specific heterobifunctional photoactivated reagents
Tóm tắt
It is shown that reagents containing one reactive amino group and one azide group crosslink chlorophyll to protein in situ. Using the light-harvesting complex of photosystem II as model system a small fraction of the chlorophylla, but no chlorophyllb was covalently bound to the protein. This confirms that chlorophylla is more accessible from the medium. 4-Azidoaniline, 4-azido-2-nitroaniline and 2-azido-4-chloroaniline are equally effective crosslinkers and are recommended as probes for the chlorophyll organization in native or reconstituted chlorophyll-protein complexes.
Tài liệu tham khảo
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