In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Wiley - Tập 11 Số 13 - Trang 2171-2178 - 2018
Sam D. Molenaar1,2, Tom Sleutels2, João Pereira2, M. Iorio2, Casper Borsje1,2, Julian A. Zamudio1,2, Francisco Fabregat‐Santiago3, Cees J.N. Buisman1,2, Annemiek ter Heijne1
1Sub-department of Environmental Technology, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands
2Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands
3Institute of Advanced Materials, Departament de Física Universitat Jaume I Av. Sos Baynat s/n 12006 Castelló de la Plana Spain

Tóm tắt

AbstractDetailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real‐time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well‐established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mg  g −1 at an anode potential of −0.35 V versus Ag/AgCl. Time‐lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in‐depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques.

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Wiley

Tập 11 Số 13

2171-2178

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