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Phân tích in silico các đột biến liên quan đến nhiễm virus viêm gan B ẩn (HBV) tại Nam Phi
Tóm tắt
Nhiễm virus viêm gan B ẩn (OBI) được định nghĩa bởi sự hiện diện của DNA virus trong gan và/hoặc huyết thanh mà không có kháng nguyên bề mặt viêm gan B (HBsAg). Trong khi nhiều nghiên cứu đã xác định các đột biến liên quan đến OBI, chỉ một phần nhỏ trong số các đột biến này đã được đặc trưng chức năng in vitro. Sử dụng các phương pháp in silico bổ sung, các tác động của các đột biến axit amin liên quan đến OBI đối với chức năng protein HBV ở những bệnh nhân HIV/HBV dương tính chưa điều trị ART tại Nam Phi đã được đánh giá. Hai đột biến liên quan đến OBI trong vùng PreS1, một trong vùng PreS2, và bảy trong vùng bề mặt của các trình tự phân nhánh phụ A1 đã được xác định là có hại. Trong các trình tự phân nhánh phụ A2, 11 đột biến liên quan đến OBI trong vùng PreS1, bảy trong vùng PreS2, và 31 trong vùng bề mặt đã được xác định là có hại. Trong vùng polymerase, 14 đột biến liên quan đến OBI trong phân nhánh phụ A1 và 71 đột biến liên quan đến OBI trong phân nhánh phụ A2 đã được xác định là có hại. Nghiên cứu này đã sử dụng các phương pháp in silico để đặc trưng hóa những tác động khả dĩ của các đột biến liên quan đến OBI đối với chức năng virus, từ đó xác định và ưu tiên các ứng cử viên, đồng thời giảm thiểu chi phí đáng kể liên quan đến các nghiên cứu chức năng cần thiết cho các nghiên cứu cơ chế của kiểu hình OBI.
Từ khóa
#nhiễm virus viêm gan B ẩn #OBI #đột biến axit amin #Nam Phi #phân tích in silico #chức năng proteinTài liệu tham khảo
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