In‐house reference (IHR) preparation of Candida albicans allergen extract A standardized extraction procedure

Allergy: European Journal of Allergy and Clinical Immunology - Tập 53 Số 4 - Trang 359-366 - 1998
Johannes Savolainen1,2,3,4, K Kalimo5, R. Einarsson6, A Koivikko3, M. Viander2, E. O. Terho7
1Department of MediCity Research Laboratory, University of Turku, Turku, Finland
2Department of Medical Microbiology, University of Turku, Turku, Finland
3Department of Pulmonary Diseases and Clinical Allergology, University of Turku, Turku, Finland
4Hôpital Arnaud de Villeneuve, Montpellier, France
5Department of Dermatology, University of Turku, Turku, Finland
6University of Linköping, Linköping, Sweden
7Department of Pediatrics, University of Turku, Turku, Finland

Tóm tắt

A standardized, controlled procedure for preparation of an in‐house reference (IHR) preparation of an allergen extract of Candida albicans is described. The procedure, based on previous studies of allergens of C albicans, is designed to yield a maximum of allergens in optimum extraction conditions and to provide a reference preparation for further extract production. The SDS‐PAGE. igE‐immunoblotting, and crossed radioimmunoelectrophoresis (CRIE) analyses showed that the procedure is reproducible with acceptable batch‐to‐batch variation. The variation in the content of the most important allergens, namely, proteins with molecular weights of 46,29, and 27 kDa in the pooled final batches, is acceptable (coeff. of variation <15%), although in the intermediate batches of different strains, the coefficient of variation may occasionally exceed 20%. A comparison with other C. albicans allergen preparations used in our previous studies is also presented. The resulting extract can be used as a reference in further extract production and also in experimental in vitro and in vivo studies.

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Thông tin xuất bản

Allergy: European Journal of Allergy and Clinical Immunology

Tập 53 Số 4

359-366

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