In-cell Western™ detection of organic cation transporters in bronchial epithelial cell layers cultured at an air–liquid interface on Transwell ® inserts

Manali Mukherjee1, M.L. Latif2, D.I. Pritchard3, C. Bosquillon1
1Division of Drug Delivery and Tissue Engineering, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
2School of Biomedical Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH, United Kingdom
3Immune Modulation Research Group, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom

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Arredondo, 2008, Receptor-mediated tobacco toxicity: Acceleration of sequential expression of alpha5 and alpha7 nicotinic receptor subunits in oral keratinocytes exposed to cigarette smoke, The FASEB Journal, 22, 1356, 10.1096/fj.07-9965.com Bleasby, 2006, Expression profiles of 50 xenobiotic transporter genes in humans and pre-clinical species: A resource for investigations into drug disposition, Xenobiotica, 36, 963, 10.1080/00498250600861751 Bouzeghrane, 2005, Enhanced expression of fibrillin-1, a constituent of the myocardial extracellular matrix in fibrosis, American Journal of Physiology Heart and Circulation Physiology, 289, H982, 10.1152/ajpheart.00151.2005 Ciarimboli, 2008, Organic cation transporters, Xenobiotica, 38, 936, 10.1080/00498250701882482 D'Argenio, 2010, Colon OCTN2 gene expression is up-regulated by peroxisome proliferator-activated receptor gamma in humans and mice and contributes to local and systemic carnitine homeostasis, Journal of Biological Chemistry, 285, 27078, 10.1074/jbc.M110.109678 Dickey, 2006, HSP induction mediates selective clearance of tau phosphorylated at proline-directed Ser/Thr sites but not KXGS (MARK) sites, The FASEB Journal, 20, 753, 10.1096/fj.05-5343fje Eder, 2010, The role of peroxisome proliferator-activated receptor alpha in transcriptional regulation of novel organic cation transporters, European Journal of Pharmacology, 628, 1, 10.1016/j.ejphar.2009.11.042 Egorina, 2006, In-cell Western assay: A new approach to visualize tissue factor in human monocytes, Journal of Thrombosis and Haemostasis, 4, 614, 10.1111/j.1538-7836.2005.01781.x Ehrhardt, 2005, Salbutamol is actively absorbed across human bronchial epithelial cell layers, Pulmonary Pharmacology & Therapeutics, 18, 165, 10.1016/j.pupt.2004.11.007 Fairbank, 2008, Airway smooth muscle cell tone amplifies contractile function in the presence of chronic cyclic strain, American Journal of Physiology. Lung Cellular and Molecular Physiology, 295, L479, 10.1152/ajplung.00421.2007 Grainger, 2006, Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier, Pharmaceutical Research, 23, 1482, 10.1007/s11095-006-0255-0 Heemskerk, 2008, Nitric oxide down-regulates the expression of organic cation transporters (OCT) 1 and 2 in rat kidney during endotoxemia, European Journal of Pharmacology, 584, 390, 10.1016/j.ejphar.2008.02.006 Horvath, 2007, Epithelial organic cation transporters ensure pH-dependent drug absorption in the airway, American Journal of Respiratory Cell and Molecular Biology, 36, 53, 10.1165/rcmb.2006-0230OC Jiang, 2009, Role of activated endocannabinoid system in regulation of cellular cholesterol metabolism in macrophages, Cardiovascular Research, 81, 805, 10.1093/cvr/cvn344 Koepsell, 2007, Polyspecific organic cation transporters: Structure, function, physiological roles, and biopharmaceutical implications, Pharmaceutical Research, 24, 1227, 10.1007/s11095-007-9254-z Kummer, 2008, The epithelial cholinergic system of the airways, Histochemistry and Cell Biology, 130, 219, 10.1007/s00418-008-0455-2 Lin, 2007, Air–liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies, Journal of Pharmaceutical Sciences, 96, 341, 10.1002/jps.20803 Lips, 2007, Down-regulation of the non-neuronal acetylcholine synthesis and release machinery in acute allergic airway inflammation of rat and mouse, Life Sciences, 80, 2263, 10.1016/j.lfs.2007.01.026 Lips, 2005, Polyspecific cation transporters mediate luminal release of acetylcholine from bronchial epithelium, American Journal of Respiratory Cell and Molecular Biology, 33, 79, 10.1165/rcmb.2004-0363OC Macdonald, 2013, Characterization of Calu-3 cell monolayers as a model of bronchial epithelial transport: Organic cation interaction studies, Journal of Drug Targeting, 21, 97, 10.3109/1061186X.2012.731068 Montagut, 2008, Elevated CRAF as a potential mechanism of acquired resistance to BRAF inhibition in melanoma, Cancer Research, 68, 4853, 10.1158/0008-5472.CAN-07-6787 Mukherjee, 2011, Variations in the expression of organic cation transporters (OCT) in bronchial epithelial cells following exposure to house dust components, Clinical and Experimental Allergy, 41, 1832 Mukherjee, 2012, Evaluation of air-interfaced Calu-3 cell layers for investigation of inhaled drug interactions with organic cation transporters in vitro, International Journal of Pharmaceutics, 426, 7, 10.1016/j.ijpharm.2011.12.036 Nakamura, 2010, Transport of ipratropium, an anti-chronic obstructive pulmonary disease drug, is mediated by organic cation/carnitine transporters in human bronchial epithelial cells: Implications for carrier-mediated pulmonary absorption, Molecular Pharmaceutics, 7, 187, 10.1021/mp900206j Nies, 2011, Organic cation transporters (OCTs, MATEs). In vitro and in vivo evidence for the importance in drug therapy, Handbook of Experimental Pharmacology, 201, 105, 10.1007/978-3-642-14541-4_3 Patonay, 1991, Near-infrared fluorogenic labels: New approach to an old problem, Analytical Chemistry, 63, 321A, 10.1021/ac00006a716 Salomon, 2012, Transport of the fluorescent organic cation 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP+) in human respiratory epithelial cells, European Journal of Pharmaceutics and Biopharmaceutics, 81, 351, 10.1016/j.ejpb.2012.03.001 Schneider, 2011, Nitric oxide-induced regulation of renal organic cation transport after renal ischemia-reperfusion injury, American Journal of Physiology. Renal Physiology, 301, F997, 10.1152/ajprenal.00264.2011 Theiss, 2005, Tumor necrosis factor (TNF) alpha increases collagen accumulation and proliferation in intestinal myofibroblasts via TNF receptor 2, Journal of Biological Chemistry, 280, 36099, 10.1074/jbc.M505291200