In Vivo Imaging of miR-221 Biogenesis in Papillary Thyroid Carcinoma

Molecular Imaging and Biology - Tập 11 - Trang 71-78 - 2008
Hyun Joo Kim1,2, June-Key Chung1,2, Do Won Hwang1,2, Dong Soo Lee1, Soonhag Kim1,3
1Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul, South Korea
2Laboratory of Molecular Imaging and Therapy of Cancer Research Institute, College of Medicine, Seoul National University, Seoul, South Korea
3Medical Research Center, College of Medicine, Seoul National University, Seoul, South Korea

Tóm tắt

To investigate the overexpression of miR-221 in papillary thyroid carcinoma (PTC), we developed a Gaussia luciferase (Gluc) system regulated by miR-221. Quantities of primary or mature miR-221 in normal thyroid cells (HT-ori3) and in PTC (NPA, TPC-1) were measured by quantitative real-time polymerase chain reaction. Cytomegalovirus (CMV)/Gluc-3xPT_miR221, which included three perfect complementary target sequences repeats of miR221 in the 3′-untranslated region of Gluc, was transfected into cells with pre-miR-221 or anti-miR-221 and Gluc activities were then compared in vitro and in vivo. Primary or mature miR-221 were overexpressed in PTC as compared with HT-ori3. In cells transfected with the Gaussia luciferase reporter system (CMV/Gluc-3xPT_miR221), Gluc activities were regulated according to miR-221 levels in vitro and in vivo. These results suggest that the devised CMV/Gluc-3xPT_miR221 system may be a useful tool for monitoring quantities of endogenous miR-221 in cells or living organisms.

Tài liệu tham khảo

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