Improving the Biodegradability of Scenedesmus obtusiusculus by Thermochemical Pretreatment to Produce Hydrogen and Methane
Tóm tắt
Hydrolysis can be considered as the bottleneck for biofuel production from microalgae due to the recalcitrance of this type of biomass. Thermochemical pretreatment is an effective and widely used process to solubilize different types of biomass. Nevertheless, the variability of the cell wall composition, among different microalgae species, hinders the development of a suitable pretreatment. In this work, the most relevant factors for thermochemical pretreatment to solubilize organic matter from microalgal biomass (Scenedesmus obtusiusculus) were analyzed and identified. The aim was to obtain hydrolysates, rich in soluble organic matter, that allow attaining higher hydrogen and methane yields than with raw microalgae, using a factorial design to identify the significant factors of the thermochemical pretreatment. The results showed that temperature (100 °C) and HCl concentration (3%) were the most significant factors to achieve carbohydrates solubilization close to 100%. Further, reaction time (1.7 h) and biomass concentration (30 g total solids/L) were also important to achieve up to 60% chemical oxygen demand (COD) solubilization. Overall, the microalgal hydrolysates produced 48 NmL H2/g VS and 296 NmL CH4/g VS, which is 1.7- and 1.3-fold higher hydrogen and methane yields, respectively, than raw microalgae. These results are meaningful because high soluble carbohydrates and COD would allow the application of high organic loading rates in continuous reactors for biofuel production using renewable biomass such as microalgae.
Tài liệu tham khảo
Deprá MC, dos Santos AM, Severo IA et al (2018) Microalgal biorefineries for bioenergy production: can we move from concept to industrial reality? Bioenergy Res 11:727–747
Carrillo-Reyes J, Buitrón G (2016) Biohydrogen and methane production via a two-step process using an acid pretreated native microalgae consortium. Bioresour Technol 221:324–330
Chen CY, Zhao XQ, Yen HW et al (2013) Microalgae-based carbohydrates for biofuel production. Biochem Eng J 78:1–10
Yap BHJ, Crawford SA, Dagastine RR, Scales PJ, Martin GJ (2016) Nitrogen deprivation of microalgae: effect on cell size, cell wall thickness, cell strength, and resistance to mechanical disruption. J Ind Microbiol Biotechnol 43:1671–1680
Martín Juárez J, Riol Pastor E, Fernández Sevilla JM, Muñoz Torre R, García-Encina PA, Bolado Rodríguez S (2018) Effect of pretreatments on biogas production from microalgae biomass grown in pig manure treatment plants. Bioresour Technol 257:30–38
Passos F, Carretero J, Ferrer I (2015) Comparing pretreatment methods for improving microalgae anaerobic digestion: thermal, hydrothermal, microwave and ultrasound. Chem Eng J 279:667–672
Kumar G, Sivagurunathan P, Thi NBD et al (2016) Evaluation of different pretreatments on organic matter solubilization and hydrogen fermentation of mixed microalgae consortia. Int J Hydrog Energy 41:21628–21640
Cheng J, Zhang M, Song W et al (2011) Cogeneration of hydrogen and methane from Arthrospira maxima biomass with bacteria domestication and enzymatic hydrolysis. Int J Hydrog Energy 36:1474–1481
Park C, Lee JH, Yang X, Yoo HY, Lee JH, Lee SK, Kim SW (2016) Enhancement of hydrolysis of Chlorella vulgaris by hydrochloric acid. Bioprocess Biosyst Eng 39:1015–1021
Hernández D, Riaño B, Coca M, García-González MC (2015) Saccharification of carbohydrates in microalgal biomass by physical, chemical and enzymatic pre-treatments as a previous step for bioethanol production. Chem Eng J 262:939–945
Solé-Bundó M, Carrère H, Garfí M, Ferrer I (2017) Enhancement of microalgae anaerobic digestion by thermo-alkaline pretreatment with lime (CaO). Algal Res 24:199–206
Dong T, Van Wychen S, Nagle N et al (2016) Impact of biochemical composition on susceptibility of algal biomass to acid-catalyzed pretreatment for sugar and lipid recovery. Algal Res 18:69–77
Toledo-Cervantes A, Morales M, Novelo E, Revah S (2013) Carbon dioxide fixation and lipid storage by Scenedesmus obtusiusculus. Bioresour Technol 130:652–658
Cortés-Carmona M, Tapia-Rodríguez A, Morales M et al (2018) Methane production from thermally pretreated Scenedesmus obtusiusculus biomass in semi-batch reactors at low reaction times. Biochem Eng J 136:61–68
Montgomery DC (2012) Design and analysis of experiments. John Wiley & Sons
Carrillo-Reyes J, Tapia-Rodríguez A, Buitrón G et al (2019) A standardized biohydrogen potential protocol: an international round robin test approach. Int J Hydrog Energy 44:26237–26247
Zahan Z, Othman MZ, Muster TH (2018) Anaerobic digestion/co-digestion kinetic potentials of different agro-industrial wastes: a comparative batch study for C/N optimisation. Waste Manag 71:663–674
Angelidaki I, Alves M, Bolzonella D, Borzacconi L, Campos JL, Guwy AJ, Kalyuzhnyi S, Jenicek P, van Lier J (2009) Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Sci Technol 59:927–934
Toledo-Cervantes A, Garduño Solórzano G, Campos JE et al (2018) Characterization of Scenedesmus obtusiusculus AT-UAM for high-energy molecules accumulation: deeper insight into biotechnological potential of strains of the same species. Biotechnol Rep 17:16–23
APHA (1999) Standard methods for the examination of water and wastewater
DuBois M, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Sivagurunathan P, Kumar G, Kobayashi T et al (2017) Effects of various dilute acid pretreatments on the biochemical hydrogen production potential of marine macroalgal biomass. Int J Hydrog Energy 42:27600–27606
Deng C, Lin R, Cheng J, Murphy JD (2019) Can acid pre-treatment enhance biohydrogen and biomethane production from grass silage in single-stage and two-stage fermentation processes? Energy Convers Manag 195:738–747
Palomo-Briones R, Razo-Flores E, Bernet N, Trably E (2017) Dark-fermentative biohydrogen pathways and microbial networks in continuous stirred tank reactors: novel insights on their control. Appl Energy 198:77–87
Bárcenas-Ruiz CD, Carrillo-Reyes J, Arellano-García L et al (2016) Pretreatment and upward liquid velocity effects over granulation in hydrogen producing EGSB reactors. Biochem Eng J 107:75–84
Liu CH, Chang CY, Cheng CL et al (2012) Fermentative hydrogen production by Clostridium butyricum CGS5 using carbohydrate-rich microalgal biomass as feedstock. Int J Hydrog Energy 37:15458–15464
González-Fernández C, Sialve B, Bernet N, Steyer JP (2012) Thermal pretreatment to improve methane production of Scenedesmus biomass. Biomass Bioenergy 40:105–111
González-Fernández C, Sialve B, Bernet N, Steyer JP (2013) Effect of organic loading rate on anaerobic digestion of thermally pretreated Scenedesmus sp. biomass. Bioresour Technol 129:219–223
Kumar G, Sivagurunathan P, Zhen G, Kobayashi T, Kim SH, Xu K (2017) Combined pretreatment of electrolysis and ultra-sonication towards enhancing solubilization and methane production from mixed microalgae biomass. Bioresour Technol 245:196–200
Santos NO, Oliveira SM, Alves LC, Cammarota MC (2014) Methane production from marine microalgae Isochrysis galbana. Bioresour Technol 157:60–67
Zhou J, Yang J, Yu Q, Yong X, Xie X, Zhang L, Wei P, Jia H (2017) Different organic loading rates on the biogas production during the anaerobic digestion of rice straw: a pilot study. Bioresour Technol 244:865–871
Yang Z, Guo R, Xu X et al (2010) Enhanced hydrogen production from lipid-extracted microalgal biomass residues through pretreatment. Int J Hydrog Energy 35:9618–9623
Yun YM, Jung KW, Kim DH et al (2012) Microalgal biomass as a feedstock for bio-hydrogen production. Int J Hydrog Energy 37:15533–15539
Passos F, Felix L, Rocha H, Pereira Jde O, de Aquino S (2016) Reuse of microalgae grown in full-scale wastewater treatment ponds: thermochemical pretreatment and biogas production. Bioresour Technol 209:305–312
Wang S, Hou X, Su H (2017) Exploration of the relationship between biogas production and microbial community under high salinity conditions. Sci Rep 7:1149
Wieczorek N, Kucuker MA, Kuchta K (2014) Fermentative hydrogen and methane production from microalgal biomass (Chlorella vulgaris) in a two-stage combined process. Appl Energy 132:108–117