Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors

Wiley - Tập 9 Số 2 - Trang 230-249 - 2011
Sarah Morran1,2, Omid Eini1,2, Tatiana Pyvovarenko1,2, Boris Parent1,2, Rohan Singh1,2, Ainur Ismagul1,2, Serik Eliby1,2, Neil J. Shirley1,2, Peter Langridge1,2, Sergiy Lopato1,2
1Australian Center for Plant Functional Genomics
2University of Adelaide

Tóm tắt

SummaryTranscription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in the agriculturally important species, wheat and barley, with a view to increase drought and frost tolerance. Transgenic wheat and barley plants were generated showing constitutive (double 35S) and drought‐inducible (maizeRab17) expression of theTaDREB2andTaDREB3transcription factors isolated from wheat grain. Transgenic populations with constitutive over‐expression showed slower growth, delayed flowering and lower grain yields relative to the nontransgenic controls. However, both theTaDREB2andTaDREB3transgenic plants showed improved survival under severe drought conditions relative to nontransgenic controls. There were two components to the drought tolerance: real (activation of drought‐stress‐inducible genes) and ‘seeming’ (consumption of less water as a result of smaller size and/or slower growth of transgenics compared to controls). The undesired changes in plant development associated with the ‘seeming’ component of tolerance could be alleviated by using a drought‐inducible promoter. In addition to drought tolerance, bothTaDREB2andTaDREB3transgenic plants with constitutive over‐expression of the transgene showed a significant improvement in frost tolerance. The increased expression ofTaDREB2andTaDREB3lead to elevated expression in the transgenics of 10 otherCBF/DREBgenes and a large number of stress responsiveLEA/COR/DHNgenes known to be responsible for the protection of cell from damage and desiccation under stress.

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Thông tin xuất bản

Wiley

Tập 9 Số 2

230-249

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