Improvement of memory by dieckol and phlorofucofuroeckol in ethanol-treated mice: Possible involvement of the inhibition of acetylcholinesterase
Tóm tắt
Phlorotannins, the polyphonic compounds found in brownEisenia andEcklonia algae, have several pharmacologically beneficial effects such as anti-inflammation. In addition, our recent data show that these compounds may improve the cognitive functions of aged humans suggesting the potential ability to enhance memory in several neurodegenerative disorders. To examine the experimental hypothesis that two effective components ofEcklonia cava, dieckol and phlorofucofuroeckol (PFF), have memory-enhancing abilities, both were administered orally to mice before a passive avoidance test. The repeated administration of either dieckol or PFF dose-dependently reduced the inhibition of latency by the administration of ethanol. To investigate the mode of memory-enhancing actions, the levels of major central neurotransmitters in three different regions (striatum, hippocampus, and frontal cortex) of the mouse brain were measured. The levels of some of the neurotransmitters, were significantly changed by ethanol. Both dieckol and PFF altered the levels of some neurotransmitters modified by the ethanol treatment. It is noteworthy that both dieckol and PFF increased the level of acetylcholine, and they exerted anticholinesterase activities. Overall, the memory-enhancing abilities of dieckol and PFF may result from, at least in part, the increment of the brain level of acetylcholine by inhibiting acetylcholinesterase.
Tài liệu tham khảo
Arendt, T., Allen, Y., Sinden, J., Schugens, M. M., Merchbanks, R. M., Lantos, P. L., and Gray, J. A., Cholinergic-rich brain transplants reverse alcohol-induced memory deficits.Nature, 332, 448–450 (1988).
Arendt, T., Holzer, M., Fruth, R., Bruckner, M. K., and Gartner, V., Paired helical filament-like phosphorylation of tau, deposition of beta/A4-amyloid and memory impairment in rat induced by chronic inhibition of phosphatase 1 and 2A.Neuroscience, 69, 691–698 (1995).
Ellman, G. L., Courtney, K. D., Andres, V. Jr., and Featherstone, R. M., A new and rapid colorimetric determination of acetylcholinesterase activity.Biochem. Pharmacol., 7, 88–95 (1961).
Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Kido, M., Mori, H., Nakayama, Y., and Takahashi, M., Structure of an antiplasmin inhibitor, eckol, isolated from the brown alga Ecklonia kurome Okamura and inhibitory activities of its derivatives on plasma plasmin inhibitors.Chem. Pharm. Bull., 37, 349–353 (1989).
Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Mori, H., Nakayama, Y., and Takahashi, M., Anti-plasmin inhibitor. VI. Structure of phlorofucofuroeckol A, a novel phlorotannin with both dibenzo-1,4-dioxin and dibenzofuran elements, from Ecklonia kurome Okamura.Chem. Pharm. Bull., 38, 133–135 (1990).
Glombitza, K.-W. and Gerstberger, G., Phlorotannins with dibenzodioxin structural elements from the brown alga Eisenia arborea.Phytochem., 24, 543–551 (1985).
Glowinski, J. and Iversen, L. L., Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.J. Neurochem., 13, 665–669 (1966).
Guerri, C. and Renau-Piqueras, J., Alcohol, astroglia, and brain development.Mol. Neurobiol. 15, 65–81 (1997).
Hiramatsu, M., Murasawa, H., Nabeshima, T., and Kameyama, T., Effects of U-50,488H on scopolamine-, mecamylamine-and dizocilpine-induced learning and memory impairment in rats.J. Pharmacol. Exp. Ther., 284, 858–867 (1998).
Kang, K., Park, Y., Hwang, H. J., Kim, S. H., Lee, J. G., and Shin, H.-C., Antioxidative properties of brown algae polyphenolics and their perspectives as chemopreventive agents against vascular risk factors.Arch. Pharm. Res., 26, 286–293 (2003).
Lee, S. C., Kim, E. J., You, K. H., Kang, J. S., and Moon, Y. S., Effects of various nootropic candidates on the impaired acquisition of ethanol-treated rats in step-thorugh test.J. Ginseng Res., 23, 115–121 (1999).
Linh, P. T., Lee, S. C., Kim, Y. H., Hong, S. P., Song, C. W., and Kang, J. S., Effects of some crude drug extracts on the brain neurotransmitters in the ethanol-treated rats.Anal. Sci. Tech., 13, 630–635 (2000).
MacInnes, J. W. and Uphouse, L. L., Effects of alcohol on acquisition and retention of passive-avoidance conditioning in different mouse strains.J. Comp. Physiol. Psychol., 84, 398–402 (1973).
Matchett, J. A. and Erickson, C. K., Alteration of ethanol-induced changes in locomotor activity by adrenergic blockers in mice.Psychopharmacology, 52, 201–206 (1977).
Myhrer, T., Neurotransmitter systems involved in learning and memory in the rat: a meta-analysis based on studies of four behavioral tasks.Brain Res. Rev., 41, 268–287 (2003).
Nakamura, T., Nagayama, K., Uchida, K. and Tanaka, R., Antioxidant activity of phlorotannins isolated from the brown alga Eisenia bicyclins.Fisheries Sci., 62, 923–926 (1996).
Palkovits, M. and Brawnstein, M. J., Maps and guide to microdissection of the rat brain. Elservier Science Publishing, p. 3, (1988).
Piercey, M. F., Vogelsang, G. D., Franklin, S. R., and Tang, A. H., Reversal of scopolamine-induced amnesia and alterations in energy metabolism by the nootropic piracetam: implications regarding identification of brain structures involved in consolidation of memory traces.Brain Res., 424, 1–9 (1987).
Raghavendra, V. and Kulkarni, S. K., Possible antioxidant mechanism in melatonin reversal of aging and chronic ethanol-induced amnesia in plus-maze and passive avoidance memory tasks.Free Radical Biol. Med., 30, 595–602 (2001).
Renis, M., Calabrese, V., Russo, A., Calderone, A., Barcellona, M. L., and Rizza, V., Nuclear DNA strand breaks during ethanol-induced oxidative stress in rat brain.FEBS Lett., 390, 153–156 (1996).
Ryback, R. S., Facilitation and inhibition of learning and memory by alcohol.Ann. N. Y. Acad. Sci., 215, 187–194 (1973).
Sakai, T. and Kato, I., Development of fucoidan from Kagome seaweed as novel health food materials.New Food Industry, 40, 1–5 (1998).
Sakai, T. and Kato, I., Functionality and health food application of seaweed fucoidans.New Food Industry, 43, 8–12 (2001).
Shibata, T., Fujimoto, K., Nagayama, K., Yamaguchi, K., and Nakamura, T., Inhibitory activity of brown algal phlorotannins against hyaluronidase.Int. J. Food Sci. Tech., 37, 703–709 (2002).
Shibata, T., Nagayama, K., Tanaka, R., Yamaguchi, K., and Nakamura, T., Inhibitory effects of brown algal phlorotannins on secretory phospholipase A2s, lipoxygenases and cycloxygenases.J. Appl. Phycol., 15, 61–66 (2003).