Improvement of leaf K+ retention is a shared mechanism behind CeO2 and Mn3O4 nanoparticles improved rapeseed salt tolerance

Yanhui Li1, Jin Hu1, Jie Qi1, Fameng Zhao1, Jiahao Liu1, Linlin Chen1, Lu Chen2, Jiangjiang Gu2, Honghong Wu1, Zhaohu Li1
1MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
2College of Science, Huazhong Agricultural University, Wuhan 430070, China

Tóm tắt

AbstractSalinity is a global issue limiting efficient agricultural production. Nanobiotechnology has been emerged as an effective approach to improve plant salt tolerance. However, little known is about the shared mechanisms between different nanomaterials-enabled plant salt tolerance. In this study, we found that both PNC [polyacrylic acid coated nanoceria (CeO2 nanoparticles)] and PMO (polyacrylic acid coated Mn3O4 nanoparticles) nanozymes improved rapeseed salt tolerance. PNC and PMO treated rapeseed plants showed significantly fresh weight, dry weight, higher chlorophyll content, Fv/Fm, and carbon assimilation rate than control plants under salt stress. Results from confocal imaging with reactive oxygen species (ROS) fluorescent dye and histochemical staining experiments showed that the ROS over-accumulation level in PNC and PMO treated rapeseed was significantly lower than control plants under salt stress. Confocal imaging results with K+ fluorescent dye showed that significantly higher cytosolic and vacuolar K+ signals were observed in PNC and PMO treated rapeseed than control plants under salt stress. This is further confirmed by leaf K+ content data. Furthermore, we found that PNC and PMO treated rapeseed showed significantly lower cytosolic Na+ signals than control plants under salt stress. While, compared with significantly higher vacuolar Na+ signals in PNC treated plants, PMO treated rapeseed showed significantly lower vacuolar Na+ signals than control plants under salt stress. These results are further supported by qPCR results of genes of Na+ and K+ transport. Overall, our results suggest that besides maintaining ROS homeostasis, improvement of leaf K+ retention could be a shared mechanism in nano-improved plant salt tolerance.

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