Tóm tắt
Reduced development of fusaria and other undesired fungi, and improved recognition of Cylindrocladium crotalariae colonies on soil-dilution plates were accomplished by supplementing a previously used medium (sucrose–TBZ medium) with three quaternary ammonium compounds, replacing peptone with tyrosine, and increasing the concentration of sucrose to yield a water potential of −10 bars. These changes eliminated the need to treat soil with NaClO to control undesired fungi. The new medium, designated sucrose–QT medium, allowed increased recovery of C. crotalariae microsclerotia from all 16 naturally infested soils tested.
