Impact of postharvest exogenous γ-aminobutyric acid treatment on cucumber fruit in response to chilling tolerance

Physiology and Molecular Biology of Plants - Tập 23 - Trang 827-836 - 2017
Parviz Malekzadeh1, Fariba Khosravi-Nejad2, Ali Asghar Hatamnia3, Reza Sheikhakbari Mehr1
1Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran
2Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
3Department of Biology, Faculty of Sciences, Ilam University, Ilam, Iran

Tóm tắt

Low-temperature storage is generally used to extend postharvest lifetime and to inhibit decay of cucumber fruit, but it also enhances the intensity of chilling injury. The capability of γ-aminobutyric acid to enhance antioxidant enzyme activities and reduce chilling injury was studied in cucumber (Cucumis sativus L.) fruit stored at 1 °C for 5 weeks. The purpose of this study was to define if the GABA-induced modification in antioxidant system and phospholipase activity is linked to the reduced chilling injury in cold-stored cucumber fruit. Alleviation of chilling injury by GABA treatment was related to increased content of proline, endogenous GABA and enhanced activities of CAT and SOD, together with reduced activities of PLC, PLD and LOX. We suggest that PLC, LOX and PLD are associated with chilling injury initiation by involvement in a signaling pathway and membrane deterioration. Therefore the results obtained in this study suggest GABA’s potential for postharvest applications for reducing chilling injury symptom in cucumber fruit.

Tài liệu tham khảo

Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress and signal transduction. Annu Rev Plant Biol 55:373–399 Bargmann BOR, Laxalt AM, Ter Riet B, Testerink C, Merquiol E, Mosblech A, Leon-Reyes A, Pieterse CMJ, Haring MA, Heilmann I, Bartels D, Munnik T (2009) Reassessing the role of phospholipase D in the Arabidopsis wounding response. Plant Cell Environ 32(7):837–850 Benjak A, Ercisli S, Vokurka A, Maletic E, Pejic I (2005) Genetic relationships among grapevine cultivars native to Croatia, Greece and Turkey. Vitis 44(2):73–77 Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation, stress: a review. Ann Bot 91:179–194 Canan I, Gundogdu M, Seday U, Oluk CA, Karasahin Z, Eroglu EC, Yazici E, Unlu M (2016) Determination of antioxidant, total phenolic, total carotenoid, lycopene, ascorbic acid, and sugar contents of Citrus species and mandarin hybrids. Turk J Agric For 40:894–899 Cao SF, Zheng YH, Wang KT, Rui HJ, Tanga SS (2009) Effects of 1-methylcyclopropene on oxidative damage, phospholipases and chilling injury in loquat fruit. J Sci Food Agric 89:2214–2220 Celik A, Ercisli S, Turgut N (2007) Some physical, pomological and nutritional properties of kiwifruit cv. Hayward. Int J Food Sci Nutr 58(6):411–418 Chance B, Maehly AC (1955) Assay of catalases and peroxidase. Methods Enzymol 2:764–775 Chen JY, He LH, Jiang YM, Wang Y, Joyce DC, Ji ZL, Lu WJ (2008) Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit. Physiol Plant 132:318–328 Chongchatuporn U, Ketsa S, van Doorn WG (2013) Chilling injury in mango (Mangifera indica) fruit peel: relationship with ascorbic acid concentrations and antioxidant enzyme activities. Postharvest Biol Technol 86:409–417 Deewatthanawong R, Nock JF, Watkins CB (2010a) γ-Aminobutyric acid (GABA) accumulation in four strawberry cultivars in response to elevated CO2 storage. Postharvest Biol Technol 57:92–96 Deewatthanawong R, Rowell P, Watkins CB (2010b) γ-Aminobutyric acid (GABA) metabolism in CO2 treated tomatoes. Postharvest Biol Technol 57:97–105 Ding CK, Wang CY, Gross KC, Smith DL (2002) Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta 214:895–901 Ding ZS, Tian SP, Zheng XL, Zhou ZW, Xu Y (2007) Responses of reactive oxygen metabolism and quality in mango fruit to exogenous oxalic acid or salicylic acid under chilling temperature stress. Physiol Plant 130:112–121 Ercisli S, Tosun M, Duralija B, Voca S, Sengul M, Turan M (2010) Phytochemical content of some black (Morus nigra L.) and purple (Morus rubra L.) mulberry genotypes. Food Technol Biotechnol 48(1):102–106 Gupta MN, Wold F (1980) A convenient spectrophotometric assay for phospholipase D using p-nitrophenyl-phosphocholine as substrate. Lipids 15:594–596 Hatamnia AA, Rostamzad A, Hosseini M, Abbaspour N, Darvishzadeh R, Malekzadeh P, Aminzadeh BM (2016) Antioxidant capacity and phenolic composition of leaves from ten Bene (Pistacia atlantica subsp. kurdica) genotypes. Nat Prod Res 30(5):600–604. doi:10.1080/14786419.2015.1028060 Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611 Hricova A, Fejer J, Libiakova G, Szabova M, Gazo J, Gajdosova A (2016) Characterization of phenotypic and nutritional properties of valuable Amaranthus cruentus L. mutants. Turk J Agric For 40:761–771 Kinnersley AM, Turano FJ (2000) Gamma aminobutyric acid (GABA) and plant responses to stress. Crit Rev Plant Sci 19:479–509 Kurioka S, Matsuda M (1976) Phospholipase C assay using p-nitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Anal Biochem 75:281–289 Lee SH, Ahn SJ, Im YJ, Cho K, Chung GC, Cho BH, Han O (2005) Differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in fig leaf gourd and cucumber roots. Biochem Biophys Res Commun 330:1194–1198 Lurie S, Crisosto CH (2005) Chilling injury in peach and nectarine. Postharvest Biol Technol 37:195–208 Malekzadeh P (2015) Influence of exogenous application of glycinebetaineon antioxidative system and growth of salt-stressed soybean seedlings (Glycine max L.). Physiol Mol Biol Plants 20:133–137 Malekzadeh P, Khara J, Heydari R (2012) Effect of exogenous gama-aminobutyric acid on physiological tolerance of wheat seedlings exposed to chilling stress. Iran J Plant Physiol 3(1):611–617 Malekzadeh P, Khara J, Heydari R (2014) Alleviating effects of exogenous Gamma-aminobutiric acid on tomato seedling under chilling stress. Physiol Mol Biol Plants 20(1):133–137 Mao LC, Pang HG, Wang GZ, Zhu CG (2007) Phospholipase D and lipoxygenase activity of cucumber fruit in response to chilling stress. Postharvest Biol Technol 44:42–47 Nishida I, Murata N (1996) Chilling sensitivity in plants and cyanobacteria: the crucial contribution of membrane lipids. Annu Rev Plant Physiol Plant Mol Biol 47:541–568 Patterson BD, Mackae EA, Ferguson IB (1984) Estimation of hydrogen peroxide in plant extracts using titanium. Anal Biochem 139:487–492 Prasad TK (2001) Mechanisms of chilling injury and tolerance. In: Basra AR (ed) Crop responses and adaptations to temperature. Food Products Press, Binghamton, pp 1–53 Promyou S, Ketsa S, van Doorn WG (2008) Hot water treatments delay cold-induced banana peel blackening. Postharvest Biol Technol 48:132–188 Qin GZ, Meng XH, Wang Q, Tian SP (2009) Oxidative damage of mitochondrial proteins contributes to fruit senescence: a redox proteomics analysis. J Proteome Res 8:2449–2462 Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136 Rice-Evans CA, Miller NJ, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152–159 Rop O, Ercisli S, Mlcek J, Jurikova T, Hoza I (2014) Antioxidant and radical scavenging activities in fruits of 6 sea buckthorn (Hippophae rhamnoides L.) cultivars. Turk J Agric For 38(2):224–232 Rui H, Cao S, Shang H, Jin P, Wang K, Zheng Y (2010) Effects of heat treatment on internal browning and membrane fatty acid in loquat fruit in response to chilling stress. J Sci Food Agric 90:1557–1561 Sawaki Y, Iuchi S, Kobayashi Y, Ikka T, Sakurai N, Fujita M, Shinozaki K, Shibata D, Kobayashi M, Koyama H (2009) STOP1 regulates multiple genes that protect arabidopsis from proton and aluminum toxicities. Plant Physiol 150(1):281–294 Shan DP, Huang JG, Yang YT, Guo YH, Wu CA, Yang GD, Gao ZG, Zheng CC (2007) Cotton GhDREB1 increases plant tolerance to low temperature and is negatively regulated by gibberellic acid. New Phytol 176:70–81 Shang H, Cao SH, YangZ Cai Y, Zheng Y (2011) Effect of exogenous γ-Aminobutyric acid treatment on proline accumulation and chilling injury in peach fruit after long-term cold storage. J Agric Food Chem 59(4):1264–1268 Shi SQ, Shi Z, Jiang Z, Qi L, Sun X, Li C (2010) Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H2O2 and ethylene production. Plant Cell Environ 33:149–162 Sirikesorn L, Ketsa S, van Doorn WG (2013) Low temperature-induced water-soaking of Dendrobium inflorescences: relation with phospholipase D activity, thiobarbaturic-acid-staining membrane degradation products, and membrane fatty acid composition. Postharvest Biol Technol 80:47–55 Song HM, Xu XB, Wang H, Wang HZ, Tao YZ (2010) Exogenous γ-aminobutyric acid alleviates oxidative damage caused by aluminium and proton stresses on barley seedlings. J Sci Food Agric 90:1410–1416 Todd JF, Paliyath G, Thompson JE (1990) Characteristics of a membrane associated lipoxygenase in tomato fruit. Plant Physiol 94:1225–1232 Trobacher CP, Clark SM, Bozzo GG, Mullen RT, DeEll JR, Shelp BR (2013) Catabolism of GABA in apple fruit: subcellular localization and biochemical characterization of two γ-aminobutyrate transaminases. Postharvest Biol Technol 75:106–113 Vicente AR, Martínez GA, Chaves AR, Civello PM (2006) Effect of heat treatment on strawberry fruit damage and oxidative metabolism during storage. Postharvest Biol Technol 40:116–122 Wallace W, Secor J, Schrader LE (1984) Rapid accumulation of γ-aminobutyric acid and alanine in soybean leaves in response to an abrupt transfer to lower temperature, darkness, or mechanical stress. Plant Physiol 175:170–175 Wise RR, Naylor AW (1987) Chilling-enhanced photophylls, chilling-enhanced photooxidation—the peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure. Plant Physiol 83:272–277 Wolfe J (2006) Chilling injury in plants—the role of membrane lipid fluidity. Plant Cell Environ 1:241–247 Wongsheree T, Ketsa S, van Doorn WG (2009) The relationship between chilling injury and membrane damage in lemon basil (Ocimum × citriodorum) leaves. Postharvest Biol Technol 51:91–96 Xu PL, Guo YK, Bai JG, Shang L, Wang XJ (2008) Effects of long-term chilling on ultrastructure and antioxidant activity in leaves of two cucumber cultivars under low light. Physiol Plant 132:467–478 Xu WT, Peng X, Luo YB, Wang J, Guo X, Huang KL (2009) Physiological and biochemical responses of grapefruit seed extract dip on ‘Redglobe’ grape. LWT Food Sci Technol 42:471–476 Yu C, Zeng L, Sheng K, Chen F, Zhou T, Zheng X, Yu T (2014) γ-Aminobutyric acid induces resistance against Penicillium expansum by priming of defence responses in pear fruit. Food Chem 159:29–37 Zhang G, Bown AW (1997) The rapid determination of γ-aminobutyric acid. Phytochemistry 44:1007–1009 Zhang C, Fei SZ, Arora R, Hannapel DJ (2010) Ice recrystallization inhibition proteins of perennial rye-grass enhances freezing tolerance. Planta 232:155–169 Zhao Y, Qian C, Chen J, Peng Y, Mao L (2010) Responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits. J Zhejiang Univ Sci B 11(6):443–450 Zorenc Z, Veberic R, Stampar F, Koron D, Mikulic-Petkovsek M (2016) Changes in berry quality of northern highbush blueberry (Vaccinium corymbosum L.) during the harvest season. Turk J Agric For 40:855–867