Immunological evaluation of Lactobacillus casei Zhang: a newly isolated strain from koumiss in Inner Mongolia, China

BMC Immunology - 2008
Ya Tuo1, Zhang Qi-jin1, Fuliang Chu2, Justin Merritt3, Bilige Menhe1, Tiansong Sun1, Ruiting Du1, Heping Zhang1
1The Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of PR China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018, PR China
2Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
3College of Dentistry, University of Oklahoma Health Sciences Centre, 975 NE 10th St BRC364, Oklahoma City, OK, 73104-5419, USA

Tóm tắt

Abstract Background There is increasing evidence to suggest an immunomodulation function both within the intestines and systemically upon consuming probiotic species. We recently isolated a novel LAB, Lactobacillus casei Zhang (LcZhang) from koumiss. LcZhang exhibited favorable probiotic properties, such as acid resistance, bile resistance, gastrointestinal (GI) colonization ability, etc. In order to examine the immunomodulatory qualities of LcZhang, we administered LcZhang to healthy mice with varying doses of either live or heat-killed LcZhang and measured various parameters of the host immune response. Results The study was performed in four separate experiments via oral administration of live and heat-killed LcZhang to BALB/c mice for several consecutive days. We investigated the immunomodulating capacity of LcZhang in vivo by analyzing the profile of cytokines, T cell subpopulations, and immunoglobulin concentrations induced in blood serum and intestinal fluid in BALB/c mice. Only live bacteria elicited a wide range of immune responses, which include the increased production of interferon-γ (IFN-γ), and depression of tumor necrosis factor-α (TNF-α) levels. In addition, interleukin-2 (IL-2) and IL-2 receptor gene transcription increased significantly, but the proportion of T cell subsets appeared to be unaffected. We also observed that LcZhang was capable of inducing gut mucosal responses by enhancing the production of secretory Immunoglobulin A (sIgA) as well influencing the systemic immunity via the cytokines released to the circulating blood. Conclusion The present work shows that the dose-dependent administration of LcZhang is capable of influencing immune responses, implying that it may be a valuable strain for probiotic use in humans.

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Tài liệu tham khảo

Cross ML: Microbes versus microbes: immune signals generated by probiotic lactobacilli and their role in protection against microbial pathogens. FEMS Immunol Med Microbiol. 2002, 34: 245-53. 10.1111/j.1574-695X.2002.tb00632.x.

Reid G, Burton J: Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect. 2002, 4: 319-24. 10.1016/S1286-4579(02)01544-7.

Vinderola G, Matar C, Palacios J, Perdigon G: Mucosal immunomodulation by the non-bacterial fraction of milk fermented by Lactobacillus helveticus R389. International Journal of Food Microbiology. 2007, 115: 180-186. 10.1016/j.ijfoodmicro.2006.10.020.

Cross ML, Ganner A, Teilab D, Fray LM: Patterns of cytokine induction by gram-positive and gram-negative probiotic bacteria. FEMS Immunol Med Microbiol. 2004, 42: 173-80. 10.1016/j.femsim.2004.04.001.

Miettinen M, Lehtonen A, Julkunen I, Matikainen S: Lactobacilli and Streptococci activate NF-kappa B and STAT signaling pathways in human macrophages. J Immunol. 2000, 164: 3733-40.

Isolauri E, Sutas Y, Kankaanpaa P, Arvilommi H, Salminen S: Probiotics: effects on immunity. Am J Clin Nutr. 2001, 73: 444S-450S.

Mercenier A, Muller-Alouf H, Grangette C: Lactic acid bacteria as live vaccines. Curr Issues Mol Biol. 2000, 2: 17-25.

Wang J, Chen X, Liu W, Yang M, Caicike A, Zhang H: Identification of Lactobacillus from koumiss by conventional and molecular methods. Eur Food Res and Technol. 2008

Wu R, Zhang H, Menghe B: [16S rDNA sequence and cluster analysis of Lb.casei. Zhang and ZL12-1 isolated from Koumiss]. China Dairy Industry. 2005, 33: 4-9.

Xu J, Yun Y, Zhang W, Shao Y, Menghe B, Zhang H: [Fermentation properties of 4 strains of Lactobacillus casei isolated from traditionally home-made koumiss in inner mongolia of China]. China Dairy Industry. 2006, 34: 23-27.

Zhang H, Menghe B, Wang J, Sun T, Xu J, Wang L, Yun Y, Wu R: [Assessment of potential probiotic properties of L.casei Zhang strain isolated from traditionally home- made Koumiss in Inner Mongolia of China]. China Diary Industry. 2006, 34: 4-7.

Kirjavainen PV, ElNezami HS, Salminen SJ, Ahokas JT, Wright PF: Effects of orally administered viable Lactobacillus rhamnosus GG and Propionibacterium freudenreichii subsp. shermanii JS on mouse lymphocyte proliferation. Clin Diagn Lab Immunol. 1999, 6: 799-802.

Kirjavainen PV, El-Nezami HS, Salminen SJ, Ahokas JT, Wright PF: The effect of orally administered viable probiotic and dairy lactobacilli on mouse lymphocyte proliferation. FEMS Immunol Med Microbiol. 1999, 26: 131-5. 10.1111/j.1574-695X.1999.tb01380.x.

Zeuthen LH, Christensen HR, Frokiaer H: Lactic acid bacteria inducing a weak interleukin-12 and tumor necrosis factor alpha response in human dendritic cells inhibit strongly stimulating lactic acid bacteria but act synergistically with gram-negative bacteria. Clin Vaccine Immunol. 2006, 13: 365-75. 10.1128/CVI.13.3.365-375.2006.

Stagg AJ, Hart AL, Knight SC, Kamm MA: Microbial-gut interactions in health and disease. Interactions between dendritic cells and bacteria in the regulation of intestinal immunity. Best Pract Res Clin Gastroenterol. 2004, 18: 255-70. 10.1016/j.bpg.2003.10.004.

Mohamadzadeh M, Olson S, Kalina WV, Ruthel G, Demmin GL, Warfield KL, Bavari S, Klaenhammer TR: Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization. Proc Natl Acad Sci USA. 2005, 102: 2880-5. 10.1073/pnas.0500098102.

Segawa S, Nakakita Y, Takata Y, Wakita Y, Kaneko T, Kaneda H, Watari J, Yasui H: Effect of oral administration of heat-killed Lactobacillus brevis SBC8803 on total and ovalbumin-specific immunoglobulin E production through the improvement of Th1/Th2 balance. Int J Food Microbiol. 2008, 121: 1-10. 10.1016/j.ijfoodmicro.2007.10.004.

Baken KA, Ezendam J, Gremmer ER, de Klerk A, Pennings JL, Matthee B, Peijnenburg AA, van Loveren H: Evaluation of immunomodulation by Lactobacillus casei Shirota: immune function, autoimmunity and gene expression. Int J Food Microbiol. 2006, 112: 8-18. 10.1016/j.ijfoodmicro.2006.06.009.

Nagao F, Nakayama M, Muto T, Okumura K: Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci Biotechnol Biochem. 2000, 64: 2706-8. 10.1271/bbb.64.2706.

Takeda K, Okumura K: Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the human NK-cell activity. J Nutr. 2007, 137: 791S-3S.

Kaburagi T, Yamano T, Fukushima Y, Yoshino H, Mito N, Sato K: Effect of Lactobacillus johnsonii La1 on immune function and serum albumin in aged and malnourished aged mice. Nutrition. 2007, 23: 342-50. 10.1016/j.nut.2007.02.001.

Targosz M, Labuda A, Czuba P, Biedron R, Strus M, Gamian A, Marcinkiewicz J, Szymonski M: Influence of macrophage activation on their capacity to bind bacterial antigens studied with atomic force microscopy. Nanomedicine. 2006, 2: 82-8.

Fukushima Y, Kawata Y, Hara H, Terada A, Mitsuoka T: Effect of a probiotic formula on intestinal immunoglobulin A production in healthy children. Int J Food Microbiol. 1998, 42: 39-44. 10.1016/S0168-1605(98)00056-7.

Kaila M, Isolauri E, Soppi E, Virtanen E, Laine S, Arvilommi H: Enhancement of the circulating antibody secreting cell response in human diarrhea by a human Lactobacillus strain. Pediatr Res. 1992, 32: 141-4.

Wallace TD, Bradley S, Buckley ND, Green-Johnson JM: Interactions of lactic acid bacteria with human intestinal epithelial cells: effects on cytokine production. J Food Prot. 2003, 66: 466-72.

Perdigon G, Nader de Macias ME, Alvarez S, Oliver G, Pesce de Ruiz Holgado AA: Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus. J Dairy Res. 1990, 57: 255-64.

Sgorbati B, Scardovi V, Leblanc DJ: Plasmids in the genus Bifidobacterium. J Gen Microbiol. 1982, 128: 2121-31.

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