Đặc điểm miễn dịch của các kết nối khe tim của chuột đồng: Sự hiện diện của các yếu tố kháng nguyên chung trong tim của các loài động vật có xương sống khác và trong các cơ quan khác nhau

The Journal of Membrane Biology - Tập 104 - Trang 119-128 - 1988
E. Dupont1, A. El Aoumari1, S. Roustiau-Sévère1, J. P. Briand2, D. Gros1
1Laboratory of Cellular Differentiation Biology, CNRS 179, Faculty of the Sciences of Luminy, Case 901, Marseille Cedex 9, France
2Institute of Molécular and Cellular Biology, CNRS, Strasbourg, France

Tóm tắt

Các kháng thể đối với peptide tổng hợp sau, SALGKLLDKVQAY, đã được tinh chế thông qua sắc ký ái lực và được phân tích qua ELISA và miễn dịch blotting. Những kháng thể này, được chứng minh là có tính đặc hiệu với thành phần protein chính của các kết nối khe tim được tách ra từ chuột đồng: connexin 43, đã phản ứng chéo với một protein đồng dạng trong các bản sao miễn dịch của các phân đoạn trái tim của cá hồi, ếch, gà, chuột lang, chuột và chuột đồng, cho thấy sự bảo tồn tiến hóa của connexin 43 trong động vật có xương sống. Qua việc miễn dịch blotting các phân đoạn cơ quan, cũng đã chứng minh rằng các kháng thể này phản ứng chéo với các protein chính có trọng lượng phân tử 32 và 22 kD trong gan chuột đồng và chuột, của trọng lượng phân tử 41 kD trong tiểu não chuột đồng, của trọng lượng phân tử 43 kD trong tử cung, dạ dày và thận chuột đồng, của trọng lượng phân tử 46 và 70 kD trong thể thủy tinh chuột đồng, cho thấy rằng các protein này chia sẻ các epitop chung hoặc có liên quan với peptide tổng hợp và connexin 43.

Từ khóa

#kháng thể #peptide tổng hợp #connexin 43 #động vật có xương sống #miễn dịch blotting

Tài liệu tham khảo

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