Immunological activities of cationic methylan derivatives
Tóm tắt
Methylan polysaccharide was aminated to add dialkylaminoethyl and free amino groups at hydroxyl sites in the methylan backbone, and these derivatives were quaternized to produce pH-independent cationic polyelectrolytes. The immunological activities of the resulting methylan derivatives were investigated. Diethylaminoethyl (DEAE)-methylan derivatives inhibited the classical pathway of the complement system in a dose-dependent way. Quaternized DEAE-methylan exhibited the highest anticomplementary activities among the all derivatives. Anticomplementary activities increased significantly as the cationic charge of the methylan derivatives increased via aminoderivatization followed by quaternization, indicating that there is an electrostatic interaction between the methylan derivatives and the negatively charged functional residues on the cell.
Tài liệu tham khảo
Alexander EL, Provost TT, Sanders ME, Frank MM, and Joiner KA (1988) Serum complement activati on in central nervous system disease in Sjogren’s syndrome. Am J Med 85, 513–8.
Chihara G (1986) Immunopharmacology of Lentinan and the Glucans. Cancer Res, 85–96.
Katsura S, Isogai A, Onabe F, and Usuda M (1992) NMR analyses of polysaccharide derivatives containing amine groups. Carbohyd Polym 18, 283–8.
Kim SY, Oh DK, and Kim JH (1996) Production of a polysaccharide, methylan, in Methylobacterium organophilum by controlling ammoniumion. Biotechnol Lett 18, 1427–30.
Lebeault JM, Kim JH, and Choi JH (1991) Novel biological polymer. USA patent no. 5,064,759.
Lee JK, Kim SY, Oh DK, and Kim JH (1998) Metal adsorption of chemically modified polysaccharide from Methylobacterium organophilum. J Agric Chem Biotechnol 41, 209–12
Nishimura K, Nishimura S, Nishi N, Saiki I, Tokura S, and Azuma I (1984) Immunological activity of chitin and its derivatives. Vaccine 2, 93–9.
Sarin VK, Kent SB, Tam JP, and Merrifield RB (1981) Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction. Anal Biochem 117, 147–57.
Strunk RC, Eidlen DM, and Mason RJ (1988) Pulmonary alveolar type II epithelial cells synthesize and secrete proteins of the classical and alternative complement pathways. J Clin Invest 81, 1419–26.
Whistler RL and Hirase S (1961) Introduction of 3,6-anhydro rings into amylose and characterization of the products. J Org Chem 26, 4600–5.