Immunohistochemistry in Dermatopathology: A Review of the Most Commonly Used Antibodies (Part I)

Folpe, 2007, Best practices in diagnostic immunohistochemistry: pleomorphic cutaneous spindle cell tumors, Arch Pathol Lab Med, 131, 1517, 10.5858/2007-131-1517-BPIDIP Wallace, 1996, Immunohistochemistry in diagnostic dermatopathology, J Am Acad Dermatol, 34, 163, 10.1016/S0190-9622(96)80106-X Wasserman, 2009, Update on immunohistochemical methods relevant to dermatopathology, Arch Pathol Lab Med, 133, 1053, 10.5858/133.7.1053 Makin, 1984, Monoclonal antibody to cytokeratin for use in routine histopathology, J Clin Pathol, 37, 975, 10.1136/jcp.37.9.975 Han, 2010, Anticytokeratin CAM5.2 is not synonymous with CK8/18 monoclonal antibody; and anticytokeratin CAM5.2 can be a marker for cytokeratin 8 but not for cytokeratin 18 and 19, Am J Surg Pathol, 34, 595, 10.1097/PAS.0b013e3181cfbfd9 Han, 2010, Antibody to cytokeratin (CK8/CK18) is not derived from CAM5.2 clone, and anticytokeratin CAM5.2 (Becton Dickinson) is not synonymous with the antibody (CK8/CK18), Hum Pathol, 41, 616, 10.1016/j.humpath.2010.01.001 Chen, 2010, Anti-cytokeratin CAM 5.2 does not act as a surrogate of the cytokeratin 8/18 monoclonal antibody, J Cutan Pathol, 37, 1123, 10.1111/j.1600-0560.2010.01514.x Requena, 2002, Pigmented mammary Paget disease and pigmented epidermotropic metastases from breast carcinoma, Am J Dermatopathol, 24, 189, 10.1097/00000372-200206000-00001 Gray, 2001, Squamous cell carcinoma detected by high-molecular-weight cytokeratin immunostaining mimicking atypical fibroxanthoma, Arch Pathol Lab Med, 125, 799, 10.5858/2001-125-0799-SCCDBH Hoang, 2010, Immunohistochemistry in the diagnosis of cutaneous neoplasms, Future Oncol, 6, 93, 10.2217/fon.09.143 Miettinen, 1993, Keratin immunohistochemistry: update of applications and pitfalls, Pathol Annu, 28, 113 Sawada, 2010, Ectopic extramammary Paget's disease: case report and literature review, Acta Derm Venereol, 90, 502, 10.2340/00015555-0892 Perrotto, 2010, The role of immunohistochemistry in discriminating primary from secondary extramammary Paget disease, Am J Dermatopathol, 32, 137, 10.1097/DAD.0b013e3181b71481 Chu, 2002, Keratin expression in human tissues and neoplasms, Histopathology, 40, 403, 10.1046/j.1365-2559.2002.01387.x Ivan, 2007, Use of p63 expression in distinguishing primary and metastatic cutaneous adnexal neoplasms from adenocarcinoma to the skin, J Cutan Pathol, 34, 474, 10.1111/j.1600-0560.2006.00644.x Hassab-el-Naby, 1989, Mixed tumors of the skin. A histological and immunohistochemical study, Am J Dermatopathol, 11, 413, 10.1097/00000372-198910000-00003 Heyderman, 1979, A new antigen on the epithelial membrane: its immunoperoxidase localization in normal and neoplastic tissues, J Clin Pathol, 32, 35, 10.1136/jcp.32.1.35 Arrese Estrada, 1990, Inmunohistoquímica y glándulas sudoríparas, Med Cut ILA, 18, 170 Sloane, 1981, Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology, Cancer, 47, 1786, 10.1002/1097-0142(19810401)47:7<1786::AID-CNCR2820470711>3.0.CO;2-8 Heyderman, 1984, Epithelial markers in primary skin cancer: an immunoperoxidase study of the distribution of epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA) in 65 primary skin carcinomas, Histopathology, 8, 423, 10.1111/j.1365-2559.1984.tb02354.x Requena, 1992, Estudio inmunohistoquímico de las glándulas ecrinas y apocrinas. Experiencia propia y revisión crítica, Piel, 7, 313 Liegl, 2007, Mammary and extramammary Paget's disease: an immunohistochemical study of 83 cases, Histopathology, 50, 439, 10.1111/j.1365-2559.2007.02633.x González-Guerra, 2008, Estudio inmunohistoquímico de la calretinina en el folículo piloso normal y neoplasias con diferenciación folicular, Actas Dermosifiliogr, 99, 456, 10.1016/S0001-7310(08)74716-1 Poblet, 2005, The calcium-binding protein calretinin is a marker of the companion cell layer of the human hair follicle, Br J Dermatol, 152, 1316, 10.1111/j.1365-2133.2005.06603.x González-Guerra, 2012, Immunohistochemical study of calretinin in normal skin and cutaneous adnexal proliferations, Am J Dermatopathol, 34, 491, 10.1097/DAD.0b013e31823aded5 Leidinger, 2004, High level of beta-hCG simulating pregnancy in recurrent osteosarcoma: case report and review of literature, J Cancer Res Clin Oncol, 130, 357, 10.1007/s00432-004-0541-3 Watanabe, 2000, h-Caldesmon in leiomyosarcoma and tumors with smooth muscle cell-like differentiation: its specific expression in the smooth muscle cell, Hum Pathol, 30, 392, 10.1016/S0046-8177(99)90113-2 Ceballos, 2000, Is anti-h-caldesmon useful for distinguishing smooth muscle and myofibroblastic tumors, Am J Clin Pathol, 114, 746, 10.1309/K5JP-A9EN-UWN7-B5GG Fanburg-Smith, 1999, Angiomatoid malignant fibrous histiocytoma: a clinicopathologic study of 158 cases and further exploration of the myoid phenotype, Hum Pathol, 30, 1143, 10.1016/S0046-8177(99)90065-5 Hornick, 2003, Myoepithelial tumors of soft tissue: a clinicopathologic and immunohistochemical study of 101 cases with evaluation of prognostic parameters, Am J Surg Pathol, 27, 1183, 10.1097/00000478-200309000-00001 Montgomery, 2002, Leiomyosarcoma of the head and neck: a clinicopathological study, Histopathology, 40, 518, 10.1046/j.1365-2559.2002.01412.x McKenney Weiss, 2001, CD31 expression in intratumoral macrophages. A potential diagnostic pitfall, Am J Surg Pathol, 25, 1167, 10.1097/00000478-200109000-00007 Kanitakis, 1996, Expression of the CD34 antigen distinguishes Kaposi's sarcoma from pseudo-Kaposi's sarcoma (acroangiodermatitis), Br J Dermatol, 134, 44, 10.1111/j.1365-2133.1996.tb07838.x Tardio, 2009, CD34-reactive tumors of the skin. An updated review of an ever-growing list of lesions, J Cutan Pathol, 36, 89, 10.1111/j.1600-0560.2008.01212.x Kirchmann, 1994, CD34 staining pattern distinguishes basal cell carcinoma from trichoepithelioma, Arch Dermatol, 130, 589, 10.1001/archderm.1994.01690050057008 Kirchman, 1995, Use of CD34 in assessing the relationship between stroma and tumor in desmoplastic keratinocytic neoplasms, J Cutan Pathol, 22, 422, 10.1111/j.1600-0560.1995.tb00757.x Bryant, 1995, Immunostaining for CD34 to determine trichoepithelioma, Arch Dermatol, 131, 616, 10.1001/archderm.131.5.616 Swanson, 1998, Immunohistologic differential diagnosis of basal cell carcinoma, squamous cell carcinoma, and trichoepithelioma in small cutaneous biopsy specimens, J Cutan Pathol, 25, 153, 10.1111/j.1600-0560.1998.tb01708.x Corbalán-Vélez, 2009, Utilidad de la tinción inmunohistoquímica con CD34 en el diagnóstico de lesiones con diferenciación tricolemal, Actas Dermosifiliogr, 100, 738, 10.1016/S0001-7310(09)72297-5 Poblet, 1994, QBEND/10 (anti-CD34 antibody) in external rooth sheath cells and follicular tumors, J Cutan Pathol, 21, 224, 10.1111/j.1600-0560.1994.tb00264.x Illueca, 1998, Malignant proliferating trichilemmal tumours: an histopathological and immunohistochemical study of three cases with DNA ploidy and morphometric evaluation, Histopathology, 33, 542, 10.1046/j.1365-2559.1998.00549.x Poblet, 1996, Trichilemmal keratosis: a clinicopathologic and immunohistochemical study of two cases, Am J Dermatopathol, 18, 543, 10.1097/00000372-199610000-00015 Lawley, 2005, Expression of Wilms tumor 1 gene distinguishes vascular malformations from proliferative endothelial lesions, Arch Dermatol, 141, 1297, 10.1001/archderm.141.10.1297 Al Dhaybi, 2010, Differentiation of vascular tumors from vascular malformations by expression of Wilms tumor 1 gene: evaluation of 126 cases, J Am Acad Dermatol, 63, 1052, 10.1016/j.jaad.2009.12.017 Trindade, 2011, Wilms tumor 1 expression in vascular neoplasms and vascular malformations, Am J Dermatopathol, 33, 569, 10.1097/DAD.0b013e3182092527 Garrido-Ruiz, 2010, WT 1 expression in nevi and melanomas: a marker of melanocytic invasion into the dermis, J Cutan Pathol, 37, 542, 10.1111/j.1600-0560.2009.01379.x Liang, 2007, Podoplanin is a highly sensitive and specific marker to distinguish primary skin adnexal carcinomas from adenocarcinomas metastatic to skin, Am J Surg Pathol, 31, 304, 10.1097/01.pas.0000213388.47913.f1 Plaza, 2010, Value of p63 and podoplanin (D2-40) immunoreactivity in the distinction between primary cutaneous tumors and adenocarcinomas metastatic to the skin: a clinicopathologic and immunohistochemical study of 79 cases, J Cutan Pathol, 37, 403, 10.1111/j.1600-0560.2010.01517.x Doeden, 2009, Lymphatic invasion in cutaneous melanoma is associated with sentinel lymph node metastasis, J Cutan Pathol, 36, 772, 10.1111/j.1600-0560.2008.01166.x Florez-Vargas, 2008, Comparative analysis of D2-40 and LYVE-1 immunostaining in lymphatic malformations, Lymphology, 41, 103 Reis, 2005, Differential Prox-1 and CD 31 expression in mucosae, cutaneous and soft tissue vascular lesions and tumors, Pathol Res Pract, 201, 771, 10.1016/j.prp.2005.08.010 Le Huu, 2010, Expression of prox1, lymphatic endothelial nuclear transcription factor, in Kaposiform hemangioendothelioma and tufted angioma, Am J Surg Pathol, 34, 1563, 10.1097/PAS.0b013e3181f6076f Wilting, 2002, The transcription factor Prox1 is a marker for lymphatic endothelial cells in normal and diseased human tissues, FASEB J, 16, 1271, 10.1096/fj.01-1010fje Partanen, 1999, Lack of lymphatic vascular specificity of vascular endothelial growth factor receptor 3 in 185 vascular tumors, Cancer, 86, 2406, 10.1002/(SICI)1097-0142(19991201)86:11<2406::AID-CNCR31>3.0.CO;2-E Costa da Cunha, 2003, Prox-1 and VEGFR3 antibodies are superior to D2-40 in identifying endothelial cells of lymphatic malformations- A proposal of a new immunohistochemical panel to differentiate lymphatic from other vascular malformations, Pediatr Dev Pathol, 12, 187, 10.2350/08-05-0471.1 Manner, 2010, MYC high level gene amplification is a distinctive feature of angiosarcomas after irradiation or chronic lymphedema, Am J Pathol, 176, 34, 10.2353/ajpath.2010.090637 Guo, 2011, Consistent MYC and FLT4 gene amplification in radiation-induced angiosarcoma but not in other radiation-associated atypical vascular lesions, Genes Chromosomes Cancer, 50, 25, 10.1002/gcc.20827 Miettinen, 2011, ERG transcription factor as an immunohistochemical marker for vascular endothelial tumors and prostatic carcinoma, Am J Surg Pathol, 35, 432, 10.1097/PAS.0b013e318206b67b Prieto, 2008, Use of immunohistochemistry in melanocytic lesions, J Cutan Pathol, 35, 1, 10.1111/j.1600-0560.2008.01130.x El Shabrawi-Caelen, 2004, Melan A: not a helpful marker in distinction between melanoma in situ in sun-damaged skin and pigmented actinic keratosis, Am J Dermatopathol, 26, 364, 10.1097/00000372-200410000-00003 Soares Almeida, 2008, Desmoplastic malignant melanoma. A clinicopathologic analysis of 113 cases, Am J Dermatopathol, 30, 207, 10.1097/DAD.0b013e3181716e6b Nonaka, 2008, Sox10: a pan-schwannian and melanocytic marker, Am J Surg Pathol, 32, 1291, 10.1097/PAS.0b013e3181658c14 Ramos-Herberth, 2010, SOX10 immunostaining distinguishes desmoplastic melanoma from excision scar, J Cutan Pathol, 37, 944, 10.1111/j.1600-0560.2010.01568.x Argenyi, 1992, Comparative light-microscopic and immunohistochemical study of traumatic and palisaded encapsulated neuromas of the skin, Am J Dermatopathol, 14, 504, 10.1097/00000372-199212000-00003 Wang, 1999, PGP 9.5. A marker for cellular neurothekeoma, Am J Surg Pathol, 23, 1401, 10.1097/00000478-199911000-00011 Sartorius, 2010, Cutaneous PGP 9.5 distribution patterns in hidradenitis suppurativa, Arch Dermatol Res, 302, 461, 10.1007/s00403-010-1028-5