Immunohistochemical dual staining as an adjunct in assessment of mitotic activity in melanoma

Journal of Cutaneous Pathology - Tập 39 Số 3 - Trang 324-330 - 2012
Kristian Ikenberg1, Madeleine Pfaltz1, Christiane Rakozy1, Werner Kempf1
1Kempf and Pfaltz Histological Diagnostics, Zurich, Switzerland

Tóm tắt

Background: The mitotic rate was introduced as a major prognostic criterion for the staging of thin (≤1.0 mm) melanoma by the 2009 American Joint Committee on Cancer Staging and Classification (seventh edition). The detection of a single mitotic figure changes the tumor stage in thin melanoma. We sought to address the value of a dual staining to facilitate the determination of the mitotic rate and to assign the mitotic activity to melanocytes.Methods: The mitotic rate of melanoma cells was determined by dual phosphohistone‐H3 (PHH3)/Melan‐A immunohistochemistry. Results were compared with PHH3 staining alone and conventional hematoxylin and eosin (H&E)‐stained slides of 15 melanomas with a tumor thickness <1.0 mm.Results: PHH3 staining clearly labeled cells in the mitotic cell cycle. The mitotic rate in the PHH3/Melan‐A dual stain was equal to that derived by H&E staining. Time required for counting mitotic figures was significantly reduced.Conclusions: The evaluation of mitotic rate with an immunohistochemical dual stain is faster (mean 63.0%) and more reliable than evaluation by routine H&E staining alone. Dual staining immunohistochemistry may be a useful additional tool to standardize the determination of mitotic rate and may be helpful in evaluation of challenging cases.Ikenberg K, Pfaltz M, Rakozy C, Kempf W. Immunohistochemical dual staining as an adjunct in assessment of mitotic activity in melanoma.

Từ khóa


Tài liệu tham khảo

10.1200/JCO.2009.23.4799

10.1016/j.ejca.2009.10.032

10.1309/LB7RTC61B7LC6HJ6

10.1111/j.1524-4725.2011.02015.x

10.1111/j.1600-0560.2009.01487.x

10.1002/cncr.10685

10.1097/01.pas.0000202048.28203.25

10.1097/01.pas.0000141389.06925.d5

10.1097/PGP.0b013e318193df97

10.1074/jbc.274.36.25543

10.1038/sj.cr.7290276

10.1007/s004120050256

10.1111/j.1432-1033.1990.tb19199.x

10.1097/01.pas.0000183572.94140.43

10.1002/ijc.2910260206

10.1097/SLA.0b013e31819ed973

Thông tin
Thông tin xuất bản

Journal of Cutaneous Pathology

Tập 39 Số 3

324-330

Thông tin tác giả