Immunofluorescence Staining of Paraffin Sections Step by Step

Sami Zaqout1, Lena‐Luise Becker2,3,4, Angela M. Kaindl2,3,4
1Basic Medical Science Department, College of Medicine, QU Health, Qatar University, Doha, Qatar
2Charité – Universitätsmedizin Berlin, Center for Chronically Sick Children (Sozialpädiatrisches Zentrum, SPZ), Berlin, Germany
3Charité – Universitätsmedizin Berlin, Department of Pediatric Neurology, Berlin, Germany
4Charité – Universitätsmedizin Berlin, Institute of Cell and Neurobiology, Berlin, Germany

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Absolom, 1986, The nature of the antigen-antibody. (bond)and the factors affecting its association and dissociation., CRC Crit. Rev. Immunol., 6, 1

Arnold, 1996, Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens., Biotech. Histochem., 71, 224, 10.3109/10520299609117164

Becheva, 2018, Comparison between direct and indirect immunofluorescence method for determination of somatic cell count., Chem. Pap., 72, 1861, 10.1007/s11696-018-0445-3

Benson, 1996, Fixation of testicular tissue for immunohistochemical and ultrastructural examination., Andrologia, 28, 27, 10.1111/j.1439-0272.1996.tb02754.x

Boenisch, 2001, Formalin-fixed and heat-retrieved tissue antigens: a comparison of their immunoreactivity in experimental antibody diluents., Appl. Immunohistochem. Mol. Morphol., 9, 176, 10.1097/00129039-200106000-00011

Boenisch, 2005, Effect of heat-induced antigen retrieval following inconsistent formalin fixation., Appl. Immunohistochem. Mol. Morphol., 13, 283, 10.1097/01.0000146524.74402.a4

Bordeaux, 2010, Antibody validation., Biotechniques, 48, 197, 10.2144/000113382

Buchwalow, 2011, Non-specific binding of antibodies in immunohistochemistry: fallacies and facts., Sci. Rep., 1, 10.1038/srep00028

Canene-Adams, 2013, Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry., Methods Enzymol., 533, 225, 10.1016/B978-0-12-420067-8.00015-5

Cătălin, 2017, The in situ morphology of microglia is highly sensitive to the mode of tissue fixation., J. Chem. Neuroanat., 86, 59, 10.1016/j.jchemneu.2017.08.007

Daneshtalab, 2010, Troubleshooting tissue specificity and antibody selection: procedures in immunohistochemical studies., J. Pharmacol. Toxicol. Methods., 127, 10.1016/j.vascn.2009.12.002

Elias, 2003, Immunohistochemical Methods in Immunohistopathology. A Practical Approach to Diagnosis.

Ellenburg, 2020, Formalin versus bouin solution for testis biopsies: which is the better fixative?., Clin. Pathol., 13, 10.1177/2632010X19897262

Eltoum, 2001, Introduction to the theory and practice of fixation of tissues., J. Histotechnol., 24, 173, 10.1179/his.2001.24.3.173

Gandhi, 2018, A unique immunofluorescence protocol to detect protein expression in vascular tissues: tacking a long standing pathological hitch., Turk. Patoloji Derg., 34, 57

Hayat, 2002, Factors affecting antigen retrieval, Microscopy, Immunohistochemistry, and Antigen Retrieval Methods, 71, 10.1007/b112626

Hoffman, 2016, The Importance of Titrating Antibodies for Immunocytochemical Methods., Curr. Protoc. Neurosci., 76, 2.12.1, 10.1002/cpns.1

Holmseth, 2012, Specificity controls for immunocytochemistry: the antigen preadsorption test can lead to inaccurate assessment of antibody specificity., J. Histochem. Cytochem., 60, 174, 10.1369/0022155411434828

Hopwood, 1969, Fixatives and fixation: a review., Histochem. J., 1, 323, 10.1007/bf01003278

Hsi, 2001, A practical approach for evaluating new antibodies in the clinical immunohistochemistry laboratory., Arch. Pathol. Lab. Med., 125, 289, 10.1097/pai.0b013e31821b505b

Ivell, 2014, Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them., Endocrinology, 155, 676, 10.1210/en.2013-1971

Kim, 2003, An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining., J. Histochem. Cytochem., 51, 129, 10.1177/002215540305100116

Kim, 2016, Immunohistochemistry for pathologists: protocols, pitfalls, and tips., J. Pathol. Transl. Med., 50, 411, 10.4132/jptm.2016.08.08

Larsson, 1988, Immunocytochemistry: Theory and Practice.

Lawrence, 2017, Optimization of Immunostaining for Prospective Image Analysis., Methods Mol. Biol., 1606, 235, 10.1007/978-1-4939-6990-6_16

Magaki, 2019, An Introduction to the Performance of Immunohistochemistry., Methods Mol. Biol., 1897, 289, 10.1007/978-1-4939-8935-5_25

Maity, 2013, Immunostaining: detection of signaling protein location in tissues, cells and subcellular compartments., Methods Cell Biol., 113, 81, 10.1016/B978-0-12-407239-8.00005-7

Manning, 2012, Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance., PLoS One, 7, 10.1371/journal.pone.0038313

Marshall, 2017, Fluorescence as a means of colour signal enhancement., Philos. Trans. R. Soc. Lond. B Biol. Sci., 372, 10.1098/rstb.2016.0335

Mason, 2000, Double immunofluorescence labelling of routinely processed paraffin sections., J. Pathol., 191, 452, 10.1002/1096-9896(2000)9999:9999<::aid-path665>3.0.co;2-o

Nasr, 2018, Paraffin immunofluorescence: a valuable ancillary technique in renal pathology., Kidney Int. Rep., 3, 1260, 10.1016/j.ekir.2018.07.008

Niedenberger, 2018, Advanced immunostaining approaches to study early male germ cell development., Stem Cell Res., 27, 162, 10.1016/j.scr.2018.01.031

Oliver, 1999, Fixation and embedding., Methods Mol. Biol., 115, 319, 10.1385/1-59259-213-9:319

Ordonez, 1988, Effect of trypsinization on the immunostaining of formalin-fixed, paraffin-embedded tissues., Am. J. Surg. Pathol., 12, 121, 10.1097/00000478-198802000-00005

Partanen, 1980, The effect of ageing on the histochemically demonstrable catecholamines in the hypogastric (main pelvic) ganglion of the rat., Histochem. J., 12, 527, 10.1007/bf01011926

Radulescu, 2007, Blocking endogenous peroxidases: a cautionary note for immunohistochemistry., J. Cell. Mol. Med., 11, 10.1111/j.1582-4934.2007.00185.x

Ramos-Vara, 2005, Technical aspects of immunohistochemistry., Vet. Pathol., 42, 405, 10.1354/vp.42-4-405

Ramos-Vara, 2000, Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed paraffin-embedded tissues: experience with 63 markers., J. Vet. Diagn. Invest., 12, 307, 10.1177/104063870001200402

Sadeghipour, 2019, Making formalin-fixed, paraffin embedded blocks., Methods Mol. Biol., 1897, 253, 10.1007/978-1-4939-8935-5_22

Saper, 2009, A guide to the perplexed on the specificity of antibodies., J. Histochem. Cytochem., 57, 1, 10.1369/jhc.2008.952770

Scalia, 2017, Antigen masking during fixation and embedding, dissected., J. Histochem. Cytochem., 65, 5, 10.1369/0022155416673995

Schnell, 1999, Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue., J. Histochem. Cytochem., 47, 719, 10.1177/002215549904700601

Shi, 1998, Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: standardization and development., Auris. Nasus Larynx, 25, 425, 10.1016/s0385-8146(98)00042-x

Shi, 2007, Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen-retrieval technique: from experiments to hypothesis., J. Histochem. Cytochem., 55, 105, 10.1369/jhc.6p7080.2006

Skliris, 2009, Immunohistochemical validation of multiple phospho-specific epitopes for estrogen receptor alpha (ERalpha) in tissue microarrays of ERalpha positive human breast carcinomas., Breast Cancer Res. Treat., 118, 443, 10.1007/s10549-008-0267-z

Smith, 2018, Embryonic immunostaining for the tardigrade hypsibius exemplaris., Cold Spring Harb. Protoc., 2018

Sun, 2011, Sudan black B reduces autofluorescence in murine renal tissue., Arch. Pathol. Lab. Med., 135, 1335, 10.5858/arpa.2010-0549-oa

Zaglia, 2016, Optimized protocol for immunostaining of experimental GFP-expressing and human hearts., Histochem. Cell. Biol., 146, 407, 10.1007/s00418-016-1456-1

Zaqout, 2017, CDK5RAP2 is required to maintain the germ cell pool during embryonic development., Stem Cell Rep., 8, 198, 10.1016/j.stemcr.2017.01.002