Imaging retinal ganglion cells: Enabling experimental technology for clinical application

Abbott, 2013, Imaging axonal transport in the rat visual pathway, Biomed. Opt. Express, 4, 364, 10.1364/BOE.4.000364 Aman, 2001, A mutant cholera toxin B subunit that binds GM1-ganglioside but lacks immunomodulatory or toxic activity, Proc. Natl. Acad. Sci. U. S. A., 98, 8536, 10.1073/pnas.161273098 Angelucci, 1996, Anterograde axonal tracing with the subunit B of cholera toxin: a highly sensitive immunohistochemical protocol for revealing fine axonal morphology in adult and neonatal brains, J. Neurosci. Methods, 65, 101, 10.1016/0165-0270(95)00155-7 Bainbridge, 2008, Effect of gene therapy on visual function in Leber's Congenital Amaurosis, N. Engl. J. Med., 358, 2231, 10.1056/NEJMoa0802268 Barnett, 2006, Selective cell uptake of modified Tat peptide–fluorophore conjugates in rat retina in ex vivo and in vivo models, Invest. Ophthalmol. Vis. Sci., 47, 2589, 10.1167/iovs.05-1470 Barnett, 2009, Single-cell imaging of retinal ganglion cell apoptosis with a cell-penetrating, activatable peptide probe in an in vivo glaucoma model, Proc. Natl. Acad. Sci. U. S. A., 106, 9391, 10.1073/pnas.0812884106 Bodeutsch, 2000, Migration of phagocytotic cells and development of the murine intraretinal microglial network: an in vivo study using fluorescent dyes, Glia, 32, 91, 10.1002/1098-1136(200010)32:1<91::AID-GLIA90>3.0.CO;2-X Burgoyne, 2005, The optic nerve head as a biomechanical structure: a new paradigm for understanding the role of IOP-related stress and strain in the pathophysiology of glaucomatous optic nerve head damage, Prog. Retin. Eye Res., 24, 39, 10.1016/j.preteyeres.2004.06.001 Bussel, 2013, OCT for glaucoma diagnosis, screening and detection of glaucoma progression, Br. J. Ophthalmol., 98, ii15, 10.1136/bjophthalmol-2013-304326 Calkins, 2012, Critical pathogenic events underlying progression of neurodegeneration in glaucoma, Prog. Retin. Eye Res., 31, 702, 10.1016/j.preteyeres.2012.07.001 Campbell, 2010, Rapid expansion of intravitreal drug injection procedures, 2000 to 2008: a population-based analysis, Arch. Ophthalmol., 128, 359, 10.1001/archophthalmol.2010.19 Charbel Issa, 2012, Optimization of in vivo confocal autofluorescence imaging of the ocular fundus in mice and its application to models of human retinal degeneration, Invest. Ophthalmol. Vis. Sci., 53, 1066, 10.1167/iovs.11-8767 Chauhan, 1996, Confocal scanning laser tomography, Can. J. Ophthalmol., 31, 152 Chauhan, 2008, Canadian Glaucoma Study: 2. risk factors for the progression of open-angle glaucoma, Arch. Ophthalmol., 126, 1030, 10.1001/archopht.126.8.1030 Chauhan, 2012, Longitudinal in vivo imaging of retinal ganglion cells and retinal thickness changes following optic nerve injury in mice, PLoS ONE, 7, e40352, 10.1371/journal.pone.0040352 Chong, 2012, Glaucoma versus red disease: imaging and glaucoma diagnosis, Curr. Opin. Ophthalmol., 23, 79, 10.1097/ICU.0b013e32834ff431 Collaborative Normal-Tension Glaucoma Study Group, 1998, Comparison of glaucomatous progression between untreated patients with normal-tension glaucoma and patients with therapeutically reduced intraocular pressures, Am. J. Ophthalmol., 126, 487, 10.1016/S0002-9394(98)00223-2 Cong, 2005, Quantitation of mouse RGCs by direct labeling with antibodies to neurofilament, PGP 9.5, Osteopontin, and Brn3 compared to retrograde labeling with Aminostilbamidine, Invest. Ophthalmol. Vis. Sci., 46 Cook, 1994, Spike initiation and propagation in wide field transient amacrine cells of the salamander retina, J. Neurosci., 14, 3852, 10.1523/JNEUROSCI.14-06-03852.1994 Cordeiro, 2011, Imaging apoptosis in the eye, Eye, 25, 545, 10.1038/eye.2011.64 Curcio, 1990, Topography of ganglion cells in human retina, J. Comp. Neurol., 300, 5, 10.1002/cne.903000103 Dezawa, 2002, Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach, Micron, 33, 1, 10.1016/S0968-4328(01)00002-6 Drager, 1981, Ganglion cell distribution in the retina of the mouse, Invest. Ophthalmol. Vis. Sci., 20, 285 Eljarrat-Binstock, 2010, New techniques for drug delivery to the posterior eye segment, Pharm. Res., 27, 530, 10.1007/s11095-009-0042-9 Ernest, 2013, An evidence-based review of prognostic factors for glaucomatous visual field progression, Ophthalmology, 120, 512, 10.1016/j.ophtha.2012.09.005 Farah, 2005, Cell birth and death in the mouse retinal ganglion cell layer, J. Comp. Neurol., 489, 120, 10.1002/cne.20615 Feng, 2000, Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP, Neuron, 28, 41, 10.1016/S0896-6273(00)00084-2 Fite, 2002, Optic afferents to the parabrachial nucleus, Brain Res., 943, 9, 10.1016/S0006-8993(02)02421-6 Folliot, 2003, Sustained tetracycline-regulated transgene expression in vivo in rat retinal ganglion cells using a single type 2 adeno-associated viral vector, J. Gene Med., 5, 493, 10.1002/jgm.367 Galindo-Romero, 2011, Axotomy-induced retinal ganglion cell death in adult mice: quantitative and topographic time course analyses, Exp. Eye Res., 92, 377, 10.1016/j.exer.2011.02.008 Galvao, 2013, In vivo imaging of retinal ganglion cell apoptosis, Curr. Opin. Pharmacol., 13, 123, 10.1016/j.coph.2012.08.007 Garcia-Frigola, 2007, Gene delivery into mouse retinal ganglion cells by in utero electroporation, BMC Dev. Biol., 7, 10.1186/1471-213X-7-103 Germain, 2013, Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild-type and retinal degeneration 1 mice, Clin. Exp. Ophthalmol., 41, 593, 10.1111/ceo.12046 Gonatas, 1983, Endocytosis of exogenous GM1 ganglioside and cholera toxin by neuroblastoma cells, Mol. Cell. Biol., 3, 91, 10.1128/MCB.3.1.91 Gordon, 2002, The Ocular Hypertension Treatment Study: baseline factors that predict the onset of primary open-angle glaucoma, Arch. Ophthalmol., 120, 714, 10.1001/archopht.120.6.714 Gray, 2006, In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells, Opt. Express, 14, 7144, 10.1364/OE.14.007144 Gross, 2007, Cargo transport: two motors are sometimes better than one, Curr. Biol., 17, R478, 10.1016/j.cub.2007.04.025 Guo, 2010, Tracking longitudinal retinal changes in experimental ocular hypertensionusing the cSLO and spectral domain-OCT, Invest. Ophthalmol. Vis. Sci., 51, 6504, 10.1167/iovs.10-5551 Harwerth, 2006, Visual field defects and retinal ganglion cell losses in patients with glaucoma, Arch. Ophthalmol., 124, 853, 10.1001/archopht.124.6.853 Hattar, 2006, Central projections of melanopsin-expressing retinal ganglion cells in the mouse, J. Comp. Neurol., 497, 326, 10.1002/cne.20970 Hernandez, 2000, The optic nerve head in glaucoma: role of astrocytes in tissue remodeling, Prog. Retin. Eye Res., 19, 297, 10.1016/S1350-9462(99)00017-8 Higashide, 2006, In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope, Invest. Ophthalmol. Vis. Sci., 47, 2943, 10.1167/iovs.05-0708 Hirokawa, 2005, Molecular motors and mechanisms of directional transport in neurons, Nat. Rev. Neurosci., 6, 201, 10.1038/nrn1624 Humayun, 1999, Morphometric analysis of the extramacular retina from postmortem eyes with retinitis pigmentosa, Invest. Ophthalmol. Vis. Sci., 40, 143 Illing, 1981, The retinal projection to the thalamus in the cat: a quantitative investigation and a comparison with the retinotectal pathway, J. Comp. Neurol., 202, 265, 10.1002/cne.902020211 Jacobson, 2012, Gene therapy for leber congenital amaurosis caused by RPE65 mutations: safety and efficacy in 15 children and adults followed up to 3 years, Arch. Ophthalmol., 130, 9, 10.1001/archophthalmol.2011.298 Jager, 2004, Risks of intravitreous injection: a comprehensive review, Retina, 24, 676, 10.1097/00006982-200410000-00002 Jeon, 1998, The major cell populations of the mouse retina, J. Neurosci., 18, 8936, 10.1523/JNEUROSCI.18-21-08936.1998 Jeun, 2011, Engineered superparamagnetic Mn0.5Zn0.5Fe2O4 nanoparticles as a heat shock protein induction agent for ocular neuroprotection in glaucoma, Biomaterials, 32, 387, 10.1016/j.biomaterials.2010.09.016 Johnson, 2007, Cell-penetrating peptide for enhanced delivery of nucleic acids and drugs to ocular tissues including retina and cornea, Mol. Ther., 16, 107, 10.1038/sj.mt.6300324 Jonas, 2008, Short-term complications of intravitreal injections of triamcinolone and bevacizumab, Eye, 22, 590, 10.1038/eye.2008.10 Kalesnykas, 2012, Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma, Invest. Ophthalmol. Vis. Sci., 53, 3847, 10.1167/iovs.12-9712 Kass, 2002, The Ocular Hypertension Treatment Study: a randomized trial determines that topical ocular hypotensive medication delays or prevents the onset of primary open-angle glaucoma, Arch. Ophthalmol., 120, 701, 10.1001/archopht.120.6.701 Keenan, 2012, Trends over time and geographical variation in rates of intravitreal injections in England, Br. J. Ophthalmol., 96, 413, 10.1136/bjophthalmol-2011-300338 Kerrigan-Baumrind, 2000, Number of ganglion cells in glaucoma eyes compared with threshold visual field tests in the same persons, Invest. Ophthalmol. Vis. Sci., 41, 741 Kielczewski, 2005, The effect of experimental glaucoma and optic nerve transection on amacrine cells in the rat retina, Invest. Ophthalmol. Vis. Sci., 46, 3188, 10.1167/iovs.05-0321 Köbbert, 2000, Current concepts in neuroanatomical tracing, Prog. Neurobiol., 62, 327, 10.1016/S0301-0082(00)00019-8 Kolb, 1992, Neurons of the human retina: a golgi study, J. Comp. Neurol., 318, 147, 10.1002/cne.903180204 Kuno, 2010, Biodegradable intraocular therapies for retinal disorders: progress to date, Drugs Aging, 27, 117, 10.2165/11530970-000000000-00000 Kwak, 1992, Evaluation of the retinal toxicity and pharmacokinetics of dexamethasone after intravitreal injection, Arch. Ophthalmol., 110, 259, 10.1001/archopht.1992.01080140115038 Kwong, 2010, RNA binding protein with multiple splicing: a new marker for retinal ganglion cells, Invest. Ophthalmol. Vis. Sci., 51, 1052, 10.1167/iovs.09-4098 Lambert, 2011, Brimonidine prevents axonal and somatic degeneration of retinal ganglion cell neurons, Mol. Neurodegener., 6, 10.1186/1750-1326-6-4 Lebel, 2013, Real-world utilization data over 4 years of ranibizumab injections for the treatment of wet age-related macular degeneration in Canada, Value Health, 16, A510, 10.1016/j.jval.2013.08.1189 Lei, 2011, Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis, Br. J. Ophthalmol., 95, 727, 10.1136/bjo.2010.180869 Leite, 2010, Effect of disease severity on the performance of Cirrus spectral-domain OCT for glaucoma diagnosis, Invest. Ophthalmol. Vis. Sci., 51, 4104, 10.1167/iovs.09-4716 Leske, 1994, The Barbados eye study. Prevalence of open angle glaucoma, Arch. Ophthalmol., 112, 821, 10.1001/archopht.1994.01090180121046 Leske, 2003, Factors for glaucoma progression and the effect of treatment: the early manifest glaucoma trial, Arch. Ophthalmol., 121, 48, 10.1001/archopht.121.1.48 Leung, 2008, Longitudinal profile of retinal ganglion cell damage after optic nerve crush with blue-light confocal scanning laser ophthalmoscopy, Invest. Ophthalmol. Vis. Sci., 49, 4898, 10.1167/iovs.07-1447 Leung, 2008, In vivo imaging of murine retinal ganglion cells, J. Neurosci. Methods, 168, 475, 10.1016/j.jneumeth.2007.10.018 Leung, 2009, Longitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury, Br. J. Ophthalmol., 93, 964, 10.1136/bjo.2008.150482 Leung, 2011, Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells, Invest. Ophthalmol. Vis. Sci., 52, 1539, 10.1167/iovs.10-6012 Levin, 2011 Lund, 1965, Uncrossed visual pathways of hooded and albino rats, Science, 149, 1506, 10.1126/science.149.3691.1506 Maguire, 2008, Safety and efficacy of gene transfer for Leber's congenital amaurosis, N. Engl. J. Med., 358, 2240, 10.1056/NEJMoa0802315 Marschall, 2011, Optical coherence tomography—current technology and applications in clinical and biomedical research, Anal. Bioanal. Chem., 400, 2699, 10.1007/s00216-011-5008-1 Massey, 2006, Functional anatomy of the mammalian retina, 43 Matsuda, 2004, Electroporation and RNA interference in the rodent retina in vivo and in vitro, Proc. Natl. Acad. Sci. U. S. A., 101, 16, 10.1073/pnas.2235688100 Medeiros, 2001, Preservation of ganglion cell layer neurons in age-related macular degeneration, Invest. Ophthalmol. Vis. Sci., 42, 795 Mikkelsen, 1992, Visualization of efferent retinal projections by immunohistochemical identification of cholera toxin subunit B, Brain Res. Bull., 28, 619, 10.1016/0361-9230(92)90112-B Miller, 2011, Adaptive optics and the eye (super resolution OCT), Eye (Lond), 25, 321, 10.1038/eye.2011.1 Mitchell, 1996, Prevalence of open-angle glaucoma in Australia. The Blue Mountains eye study, Ophthalmology, 103, 1661, 10.1016/S0161-6420(96)30449-1 Mitrofanis, 1990, A distinctive soma size gradient among catecholaminergic neurones of human retinae, Brain Res., 527, 69, 10.1016/0006-8993(90)91061-K Murphy, 2007, Injury to retinal ganglion cell axons increases polysialylated neural cell adhesion molecule (PSA-NCAM) in the adult rodent superior colliculus, Brain Res., 1163, 21, 10.1016/j.brainres.2007.05.069 Myles, 2005, Recent progress in ocular drug delivery for posterior segment disease: emphasis on transscleral iontophoresis, Adv. Drug Deliv. Rev., 57, 2063, 10.1016/j.addr.2005.08.006 Nadal-Nicolas, 2012, Whole number, distribution and co-expression of Brn3 transcription factors in retinal ganglion cells of adult albino and pigmented rats, PLoS ONE, 7, e49830, 10.1371/journal.pone.0049830 Nema, 2013 Nickerson, 2012, Subretinal delivery and electroporation in pigmented and nonpigmented adult mouse eyes, Methods Mol. Biol., 884, 53, 10.1007/978-1-61779-848-1_4 Oglesby, 2012, Semi-automated, quantitative analysis of retinal ganglion cell morphology in mice selectively expressing yellow fluorescent protein, Exp. Eye Res., 96, 107, 10.1016/j.exer.2011.12.013 Osborne, 2001, A hypothesis to explain ganglion cell death caused by vascular insults at the optic nerve head: possible implication for the treatment of glaucoma, Br. J. Ophthalmol., 85, 1252, 10.1136/bjo.85.10.1252 Pang, 2011, Morphology and immunoreactivity of retrogradely double-labeled ganglion cells in the mouse retina, Invest. Ophthalmol. Vis. Sci., 52, 4886, 10.1167/iovs.10-5921 Pang, 2002, Relative contributions of bipolar cell and amacrine cell inputs to light responses of ON, OFF and ON-OFF retinal ganglion cells, Vis. Res., 42, 19, 10.1016/S0042-6989(01)00258-9 Pang, 2002, Segregation and integration of visual channels: layer-by-layer computation of ON-OFF signals by amacrine cell dendrites, J. Neurosci., 22, 4693, 10.1523/JNEUROSCI.22-11-04693.2002 Perry, 1981, Evidence for an amacrine cell system in the ganglion cell layer of the rat retina, Neuroscience, 6, 931, 10.1016/0306-4522(81)90174-3 Piri, 2006, Expression of hermes gene is restricted to the ganglion cells in the retina, Neurosci. Lett., 405, 40, 10.1016/j.neulet.2006.06.049 Quigley, 1996, Number of people with glaucoma worldwide, Br. J. Ophthalmol., 80, 389, 10.1136/bjo.80.5.389 Quigley, 1999, Neuronal death in glaucoma, Prog. Retin. Eye Res., 18, 39, 10.1016/S1350-9462(98)00014-7 Quigley, 2004, New paradigms in the mechanisms and management of glaucoma, Eye, 19, 1241, 10.1038/sj.eye.6701746 Quigley, 1979, The histology of human glaucoma cupping and optic nerve damage: clinicopathologic correlation in 21 eyes, Ophthalmology, 86, 1803, 10.1016/S0161-6420(79)35338-6 Resnikoff, 2004, Global data on visual impairment in the year 2002, Bull. World Health Organ., 82, 844 Rifkin, 2012, Factors affecting patients' pain intensity during in office intravitreal injection procedure, Retina, 32, 696, 10.1097/IAE.0b013e3182252ad3 Rodieck, 1979, Visual pathways, Annu. Rev. Neurosci., 2, 193, 10.1146/annurev.ne.02.030179.001205 Rodrigues, 2011, Effect of needle type and injection technique on pain level and vitreal reflux in intravitreal injection, J. Ocul. Pharmacol. Ther., 27, 197, 10.1089/jop.2010.0082 Rodriguez, 2014, The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina, J. Comp. Neurol., 522, 1411, 10.1002/cne.23521 Rosenfeld, 2005, Maximum tolerated dose of a humanized anti-vascular endothelial growth factor antibody fragment for treating neovascular age-related macular degeneration, Ophthalmology, 112, 1048, 10.1016/j.ophtha.2005.01.043 Ryu, 2011, Suppression of phagocytic cells in retinal disorders using amphiphilic poly(gamma-glutamic acid) nanoparticles containing dexamethasone, J. Control. Release, 151, 65, 10.1016/j.jconrel.2010.11.029 Saati, 2010, Mini drug pump for ophthalmic use, Curr. Eye Res., 35, 192, 10.3109/02713680903521936 Sabel, 1997, In vivo confocal neuroimaging (ICON) of CNS neurons, Nat. Med., 3, 244, 10.1038/nm0297-244 Salinas-Navarro, 2009, Retinal ganglion cell population in adult albino and pigmented mice: a computerized analysis of the entire population and its spatial distribution, Vis. Res., 49, 637, 10.1016/j.visres.2009.01.010 Salinas-Navarro, 2009, A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats, Vis. Res., 49, 115, 10.1016/j.visres.2008.09.029 Sample, 2000, Short-wavelength automated perimetry: it's role in the clinic and for understanding ganglion cell function, Prog. Retin. Eye Res., 19, 369, 10.1016/S1350-9462(00)00001-X Sanchez, 2011, Cholera toxin – a foe & a friend, Indian J. Med. Res., 133, 153 Schlamp, 2013, Evaluation of the percentage of ganglion cells in the ganglion cell layer of the rodent retina, Mol. Vis., 19, 1387 Schuman, 2008, Spectral domain optical coherence tomography for glaucoma (an AOS thesis), Trans. Am. Ophthalmol. Soc., 106, 426 Schwarz, 1996, The localization of gangliosides in neurons of the central nervous system: the use of anti-ganglioside antibodies, Biochim. Biophys. Acta (BBA) – Rev. Biomembr., 1286, 247, 10.1016/S0304-4157(96)00011-1 Simon, 1998, Combined methods of retrograde staining, layer-separation and viscoelastic cell stabilization to isolate retinal ganglion cells in adult rats, J. Neurosci. Methods, 83, 113, 10.1016/S0165-0270(98)00060-0 Somfai, 2007, Evaluation of potential image acquisition pitfalls during optical coherence tomography and their influence on retinal image segmentation, J. Biomed. Opt., 12, 041209, 10.1117/1.2774827 Spear, 1996, Relationship between numbers of retinal ganglion cells and lateral geniculate neurons in the rhesus monkey, Vis. Neurosci., 13, 199, 10.1017/S0952523800007239 Sung, 2003, Central challenges facing the national clinical research enterprise, JAMA, 289, 1278, 10.1001/jama.289.10.1278 Surgucheva, 2008, γ-Synuclein as a marker of retinal ganglion cells, Mol. Vis., 14, 1540 Taylor, 1996, Response properties of long-range axon-bearing amacrine cells in the dark-adapted rabbit retina, Vis. Neurosci., 13, 599, 10.1017/S0952523800008506 Tezel, 2008, TNF-alpha signaling in glaucomatous neurodegeneration, Prog. Brain Res., 173, 409, 10.1016/S0079-6123(08)01128-X Thanos, 2002, In vivo FM: using conventional fluorescence microscopy to monitor retinal neuronal death in vivo, Trends Neurosci., 25, 441, 10.1016/S0166-2236(02)02246-4 Tielsch, 1991, Racial variations in the prevalence of primary open-angle glaucoma. The Baltimore Eye Survey, JAMA, 266, 369, 10.1001/jama.1991.03470030069026 van Oterendorp, 2011, Light scattering and wavefront aberrations in in vivo imaging of the rat eye: a comparison study, Invest. Ophthalmol. Vis. Sci., 52, 4551, 10.1167/iovs.10-6882 Vidal-Sanz, 1987, Axonal regeneration and synapse formation in the superior colliculus by retinal ganglion cells in the adult rat, J. Neurosci., 7, 2894, 10.1523/JNEUROSCI.07-09-02894.1987 Vidal-Sanz, 2011, Understanding glaucomatous damage: anatomical and functional data from ocular hypertensive rodent retinas, Prog. Retin. Eye Res., 31, 1, 10.1016/j.preteyeres.2011.08.001 Wang, 2010, Cyan fluorescent protein (CFP) expressing cells in the retina of Thy1-CFP transgenic mice before and after optic nerve injury, Neurosci. Lett., 468, 110, 10.1016/j.neulet.2009.10.077 Weale, 2004, Toward a future for aging eyes, 205 Webb, 1987, Confocal scanning laser ophthalmoscope, Appl. Opt., 26, 1492, 10.1364/AO.26.001492 Weinreb, 2004, Primary open-angle glaucoma, Lancet, 363, 1711, 10.1016/S0140-6736(04)16257-0 Wells, 2011, Photoresponsive PEG-anthracene grafted hyaluronan as a controlled-delivery biomaterial, Biomacromolecules, 12, 923, 10.1021/bm101233m Wensor, 1998, The prevalence of glaucoma in the Melbourne Visual Impairment Project, Ophthalmology, 105, 733, 10.1016/S0161-6420(98)94031-3 Williams, 2011, Imaging single cells in the living retina, Vis. Res., 51, 1379, 10.1016/j.visres.2011.05.002 Williams, 1996, Genetic and environmental control of variation in retinal ganglion cell number in mice, J. Neurosci., 16, 7193, 10.1523/JNEUROSCI.16-22-07193.1996 Xiang, 1995, The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons, J. Neurosci., 15, 4762, 10.1523/JNEUROSCI.15-07-04762.1995 Xu, 2000, Permeability and diffusion in vitreous humor: implications for drug delivery, Pharm. Res., 17, 664, 10.1023/A:1007517912927 Yin, 2011, Intravitreal injection of AAV2 transduces macaque inner retina, Invest. Ophthalmol. Vis. Sci., 52, 2775, 10.1167/iovs.10-6250 Yu, 2009, Slow excitation of cultured rat retinal ganglion cells by activating group I metabotropic glutamate receptors, J. Neurophysiol., 102, 3728, 10.1152/jn.00650.2009 Zinser, 1989, Confocal laser tomographic scanning of the eye, Proc. SPIE, 1161, 337, 10.1117/12.962716