Image analysis as a simple and useful tool for evaluating the constitutive heterochromatin (C-banding) in human chromosomes

Human Evolution - 1993
R. Di Lernia1, D. Formenti2, A. M. Fuhrman Conti1
1Dip. di Biologia e Genetica per le Scienze Mediche, University of Milano, Milano, Italy
2Dip. di Biologia Animale, University of Pavia, Pavia, Italy

Tóm tắt

Image analysis can contribute to those fields of cytogenetics that are influenced most by subjectivity, especially evaluation of chromosome regions that are histochemically polymorphic. C-banding of human or primate chromosomes may be used as a typical example of this concept. In addition, using it quantitatively it is useful in studies of population cytogenetics of man and Primates. Therefore, the aim of this study was to determine the best conditions for measurement by image analysis of C-positive regions in a sample of 29 normal subjects. We looked for relationships of chromosomal C-positive areas with the dimensions of the corresponding metaphases. Finally, we suggest some criteria for potential use of C-banding in population and comparative studies.

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Tài liệu tham khảo

Arrighi F.E. & Hsu T. C. 1971.Localization of heterochromatin in human chromosomes. Cytogenetics, 10, 81–86.

Babu K.A. & Verma R.S. 1985a.The staining profiles of constitutive heterochromatin in human chromosomes. Genetics, 110 (NO. 3, Pt. 2), S3.

Babu K.A. & Verma R.S., 1985b.Identification of additional chromosome 18 in Edwards' syndrome using restriction endonuclease Alu I. Am. J. Hum. Genet., 37(4), A43.

Babu A. & Verma R.S., 1986a.Cytochemical heterogeneity of C-band in human chromosome 1. Histochem. J. 18, 329–333.

Babu A. & Verma R.S., 1986b.Heteromorphic variants of chromosome 4. Cytogenet. Cell Genet., 41, 60–61.

Babu A. & Verma R.S., 1986c.The heteromorphic marker on chromosome 18. Am. J. Hum. Genet., 38, 549–554.

Babu K.A. 1988. Heterogeneity of heterochromatin of human chromosomes as demonstrated by restriction endonuclease treatment. In (Ram S. Verma, ed.). Heterochromatin Molecular and structural aspects, pp. 250–275. Cambridge University Press.

Bobrow M., Madan K. & Pearson P.L., 1972.Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9. Nature, 238, 122–124.

Buys C.H.C.M., Gorw W.L., Blenkers J.A.M. & van Dalen C.H., 1981.Heterogeneity of human chromosome 9 constitutive heterochromatin as revealed by sequential distamycin A/DAPI staining and C-banding. Hum. Genet., 57, 28–30.

Donlon T. A. & Magenis R.E., 1981.Structural organization of the heterochromatic region of human chromosome 9. Chromosoma, 84, 353–363.

Erdtmann B., 1982.Aspects of evaluation, significance, and evolution of human C-band Heteromorphism. Hum. Genet., 61, 281–294.

Magenis RE., Donlon T.A. & Wyandt H.E., 1978.Giemsa-11 staining of chromosome 1: a newly described heteromorphism. Science, 202, 64–65.

Patil, S. R., & Lubs H.A., 1977.Classification of qh regions in human chromosomes 1, 9 and 16 by C-banding. Hum. Genet., 38, 35–38.

Verma R.S., 1988. Heteromorphisms of heterochromatin. In (Ram S. Verma, ed.). Heterochromatin Molecular and structural aspects, pp. 276–291. Cambridge University Press.

Wienberg J. and Stanyon R., 1987.Fluorescent heterochromatin staining in primate chromosomes. Human Evolution, Vol. 2, N. 5, 445–457).

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